Angiographic, Anatomic and Clinical Technique Descriptions of A Subcarapacial Venipuncture Site for Chelonians

Hernandez‐Divers, Sonia M.; Hernandez-Divers, Stephen J.; Wyneken, Jeanette

doi:10.5818/1529-9651.12.2.32

Cited by 84 publications

(53 citation statements)

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“…Blood samples were collected using a 2.5 cm, 22-gauge needle on a 3 ml syringe via the subcarapacial sinus (Hernandez-Divers et al ., 2002). The time to blood sample from first disturbance (upon first discovering the animal during survey before setting up a ‘processing station’ nearby) was noted upon each sampling event using a stopwatch.…”

Section: Methodsmentioning

confidence: 99%

Stress response to handling is short lived but may reflect personalities in a wild, Critically Endangered tortoise species

Currylow¹,

Louis

Crocker

2017

Conservation Physiology

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Section: Methodsmentioning

confidence: 99%

Stress response to handling is short lived but may reflect personalities in a wild, Critically Endangered tortoise species

Currylow¹,

Louis

Crocker

2017

Conservation Physiology

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“…Blood samples (1.0 mL) were collected from each tortoise via subcarapacial venipuncture (Hernandez-Divers et al 2002) using a 3.8-cm, 23-gage needle and 3 cc syringe coated with sodium heparin. An aliquot (0.5 mL) of whole blood was placed immediately into an RNeasy Animal Protect TM collection tube (Qiagen, Valencia, CA) and frozen at -20°C.…”

Section: Tissue Collectionmentioning

confidence: 99%

Integrating Gene Transcription-Based Biomarkers to Understand Desert Tortoise and Ecosystem Health

et al. 2015

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Tortoises are susceptible to a wide variety of environmental stressors, and the influence of human disturbances on health and survival of tortoises is difficult to detect. As an addition to current diagnostic methods for desert tortoises, we have developed the first leukocyte gene transcription biomarker panel for the desert tortoise (Gopherus agassizii), enhancing the ability to identify specific environmental conditions potentially linked to declining animal health. Blood leukocyte transcript profiles have the potential to identify physiologically stressed animals in lieu of clinical signs. For desert tortoises, the gene transcript profile included a combination of immune or detoxification response genes with the potential to be modified by biological or physical injury and consequently provide information on the type and magnitude of stressors present in the animal's habitat. Blood from 64 wild adult tortoises at three sites in Clark County, NV, and San Bernardino, CA, and from 19 captive tortoises in Clark County, NV, was collected and evaluated for genes indicative of physiological status. Statistical analysis using a priori groupings indicated significant differences among groups for several genes, while multidimensional scaling and cluster analyses of transcription C T values indicated strong differentiation of a large cluster and multiple outlying individual tortoises or small clusters in multidimensional space. These analyses highlight the effectiveness of the gene panel at detecting environmental perturbations as well as providing guidance in determining the health of the desert tortoise.

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“…9 Deniz kaplumbağalarında venöz kan alımı için uygun birçok vücut bölgesi tanımlanmıştır. 5,[10][11][12] Tür, boyut ve hasta durumuna göre kan örneği alım yolu tercih edilebilmektedir. Kan örneği alımında lenf kontaminasyonu olmamasına dikkat edilmesi gerekmektedir.…”

Section: Muayene Ve Taniunclassified

Emergency and Critical Care in Sea Turtles

Altuğ¹,

Deveci²,

Işler³

et al. 2017

Turkiye Klinikleri J Vet Sci

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Ö ÖZ ZE ET T Deniz kaplumbağaları olarak tanımlanan yedi türün altısı, Uluslararası Doğa Koruma Birliği (IUCN) tarafından soyu tükenme tehlikesindeki türler olarak listelenmiştir. Populasyon artışının birçok bölgede istenen düzeyde olmaması ve azalma eğiliminde olması sebebiyle koruma ve kurtarma çalışmaları önem kazanmaktadır. Çeşitli sebeplerle acil müdahale ve yoğun bakım gerektiren patolojilerle sıklıkla ölüm riskiyle karşı karşıya bulunan deniz kaplumbağalarının, özellikle de dişilerin kurtarılması önem arz etmektedir. Deniz kaplumbağalarının kabuklu ve ektotermik olmaları gibi anatomik ve fizyolojik olarak bir çok hayvandan farklı özellikler göstermesi veteriner hekimlere zorluk teşkil etmektedir. Bu çalışmada; kritik hasta ve yaralı deniz kaplumbağalarında acil müdahale ve yoğun bakım gereken durumların ve önceliğinin belirlenmesi, önemi, uygulamaların yapılışı ve dikkat edilecek noktalar hakkında bilgiler sunulması amaçlanmıştır. Muayene ve teşhis, hastanın stabilize edilmesi, tercih edilen optimal sıcaklık aralığı (TOSA), sıvı sağaltımı, oral uygulamalar, kardiyopulmoner resüsitasyon uygulamaları, terapötik ajanlar, bakım, besleme, hospitalizasyon uygulamaları gibi başlıklara dair mevcut güncel tercihler derlendi. Ayırıcı tanı ve tedavi uygulamalarındaki ilkeler ve önemli noktalara değinildi. Travmatik hasarlar, hipertermi ve hipotermi, boğulma, zehirlenme, distosi, kloaka ve fallus prolapsusları, distosi, kusma, ileus, obstrüksi-yonlar, parazitler, suya batmama sorunları, pnömoni, dekompresyon hastalığı, enfeksiyöz hastalıklar ve septisemi, bakteriyel ve fungal hastalıklar gibi önemli sorunlar hakkında özlü bilgiler verildi. Bakım şartları ve rehabilitasyonda önemli püf noktaları belirtildi. Acil müdahale ve yoğun bakım gereken kritik hasta deniz kaplumbağalarında yapılması gereken uygulamalar bütüncül bir yaklaşım içerisinde derlenerek sunuldu.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : Deniz kaplumbağaları; acil; yoğun bakım; derleme; caretta; chelonia A AB BS S T TR RA AC CT T Six of the seven species described as sea turtles were listed as endangered species by the International Union for Conservation of Nature (IUCN). Protection and rescue efforts are gaining importance as the population increase is not at the desired level in many regions and tends to decline. It is also important to save sea turtles, especially females, who have pathologies that require emergency and critical care caused by a variety of reasons and often at risk of death. It is challenging for veterinarians that sea turtles have unusual anatomical and physiological features such as the shell and being ectothermic. This article presents information on the medical evaluation and stabilization of critically ill and injured sea turtle patients presented to the emergency clinician. Determination and priorities, importance, implementation and points to be noted of emergency and critical care are reviewed. Current preferences for examination and diagnosis, patient stabilization, preferred optimal temperature zone (POTZ), f...

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Angiographic, Anatomic and Clinical Technique Descriptions of A Subcarapacial Venipuncture Site for Chelonians

Cited by 84 publications

References 1 publication

Stress response to handling is short lived but may reflect personalities in a wild, Critically Endangered tortoise species

Stress response to handling is short lived but may reflect personalities in a wild, Critically Endangered tortoise species

Integrating Gene Transcription-Based Biomarkers to Understand Desert Tortoise and Ecosystem Health

Emergency and Critical Care in Sea Turtles

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