Angiogenic Type I Collagen Extracellular Matrix Integrated with Recombinant Bacteriophages Displaying Vascular Endothelial Growth Factors

Abstract

Here, a growth-factor-integrated natural extracellular matrix of type I collagen is presented that induces angiogenesis. The developed matrix adapts type I collagen nanofibers integrated with synthetic colloidal particles of recombinant bacteriophages that display vascular endothelial growth factor (VEGF). The integration is achieved during or after gelation of the type I collagen and the matrix enables spatial delivery of VEGF into a desired region. Endothelial cells that contact the VEGF are found to invade into the matrix to form tube-like structures both in vitro and in vivo, proving the angiogenic potential of the matrix.

Fabrication of type I collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell–ECM interactions

“…In the selected condition (pH 7.4; Q cp = 1000 μl min −1 ), fabricated hydrobeads had only 4.8% of coefficient of variation (C.V., figure 2(b)), which is in an acceptable range, comparing previous references (C.V = 4.6%-6.4%) [7,8,16,17]. SEM images showed (figure 2(e)) a fibrous network of collagen structure of hydrobeads which was equivalent to the previously reported structure of COL1 matrix [26]. The hydrobeads have large inter-fiber space, for the residual surfactant easily removed in washing processes.…”

Evaluation of Cell‐Penetrating Peptides Using Microfluidic In Vitro 3D Brain Endothelial Barrier

“…[ 34–38 ] First, microfluidic platforms could provide visualization of different view during molecular transport and transmigration of cells through endothelial barrier. [ 39 ] Besides, microfluidic platforms have been amenable to adjusting extracellular matrix (ECM) hydrogel [ 40,41 ] and to observing cellular response. Although numerous previous studies have tried to build a tight endothelial barrier, only a few have facilitated their platform to evaluate BBB drugs.…”

Construction of a 3D mammary duct based on spatial localization of the extracellular matrix

“…These models were used to investigate interactions between cells and microenvironmental components of ECM. ECM hydrogel modified by growth factors or other ECM components to mimic BM 33,34 were shown to be essential for the formation of stable endothelial or epithelial barriers in in vitro models. Recombinant BM (rBM) was generated by spatial modification of COL1 nanofibers within diluted MAT 34 and formed a stable endothelial monolayer with an enhanced endothelial barrier function resulting from "outside-in" signaling between laminin and integrin.…”