Angiogenic properties of natural rubber latex biomembranes and the serum fraction of Hevea brasiliensis

Abstract

The angiogenic properties of natural rubber were evaluated in this work. We have used the chick chorioallantoic membrane assay (CAM) as a model to investigate the influence of the heating on biological activity in rubber membranes and in non rubber fraction as well. Results showed that natural rubber membranes can induce vascularization. It was observed that angiogenesis activity was maximum when membranes were heated in temperatures between 65 o C and 85 o C, considering a range from 55 o C to 105 o C. The same behavior was observed for non rubber fraction and it indicates that this serum fraction may be responsible for angiogenesis. When infrared spectroscopy was performed in the cast films of non rubber fraction samples, as a function of heating, no structural changes was observed. The results obtained shown that natural rubber latex films produced by casting induce the vessel growth in the CAM and it can be considered as a potential biomaterial.

Anatomical and functional evaluation of tympanoplasty using a transitory natural latex biomembrane implant from the rubber tree Hevea brasiliensis

“…All values were significant for each individual group regarding ABG closure, with no significant differences between groups (Table 6). Preliminary studies have shown the presence of a possible vascular growth factor in Hevea brasiliensis which acts on human tissues, permitting improved vascularization of the temporalis muscle fascia graft from the remaining tympanic membrane by means of its ability to induce angiogenesis 11,12 . The use of the latex biomembrane as a transitory implant in addition to the temporalis muscle fascia graft proved to be biocompatible, causing no increase in infections, otorrhea or otorragy compared to the control group or the silicone membrane group (Table 3).…”

“…This biocompatibility and the tissue regeneration properties of the natural latex have led to the recognition and liberation of the use of the biomembrane by the Brazilian health regulatory authority ANVISA 5,11,13,14 .…”

Evaluation on Cytotoxicity of Natural Rubber Latex Nanoparticles and Application in Bone Tissue Engineering

“…The reason behind this is well explored as allergic problems in some sensitive individuals, which cause immediate hypersensitivity, mediated by immunoglobulin E (IgE) antibodies to specific proteins (Hevb1 and Hevb3) in the latex. On the one hand, however, the prevalence of latex allergy in population is believed to be very low 4,5) .…”

Free-radical Scavenging properties and Cytotoxic Activity Evaluation of Latex C-serum from Hevea brasiliensis RRIM 600

“…6 Latex and its constituents have been specifically implicated in multiple biological propertiesand it is known to stimulate angiogenic activity, cell adhesion, extracellular matrix formation, and to improve wound healing in skin tissue. 7,8 Ferreira et al (2009), 9 showed that the non-rubber constituents of H. brasiliensis latex are the bioactive fraction responsible for angiogenic properties of this material.Latexconstituents can be separated in three parts when centrifuged at high speed, consisting of the rubber cream, centrifuged serum (C-serum) and the bottom fraction (B-serum). 7,9 The B-serum contains vacuole-like organelles known as lutoids bodies.…”

“…7,8 Ferreira et al (2009), 9 showed that the non-rubber constituents of H. brasiliensis latex are the bioactive fraction responsible for angiogenic properties of this material.Latexconstituents can be separated in three parts when centrifuged at high speed, consisting of the rubber cream, centrifuged serum (C-serum) and the bottom fraction (B-serum). 7,9 The B-serum contains vacuole-like organelles known as lutoids bodies. C-serum is the phase that contains the soluble substances found in plant cells cytoplasm such as proteins, amino acids, carbohydrates, organic acids, inorganic salts and nucleotidic materials.…”