Angiogenic factor in vitreous from diabetic retinopathy

Hill, C. R.; Kissun, R D; Weiss, Jacqueline B.; Garner, A

doi:10.1007/bf01971107

Cited by 31 publications

(15 citation statements)

References 9 publications

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“…It has been suggested that circulating and local factors may contribute to the progression of retinopathy. These factors include growth hormone [35], insulin-like growth factor-1 [36,37], and various other growth factors (eg, vascular endothelial growth factor) and other angiogenic factors isolated from the vitreous of patients with proliferative retinopathy that produce vessel proliferation in vitro [38,39]. The relevance of these findings to pregnancy is that the placenta also produces angiogenic factors that result in vessel proliferation in endothelial cell cultures in vitro [40].…”

Section: Pregnancy and Diabetic Retinopathymentioning

confidence: 87%

Microvascular complications and the diabetic pregnancy

Bond

Umans²

2006

Curr Diab Rep

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Without strict glycemic control, diabetic pregnancies are frequently complicated by spontaneous abortion, stillbirth, or congenital malformation. Retrospective studies have been largely reassuring that pregnancy does not accelerate morbid outcomes in women with diabetic vascular disease. Improved outcomes of high-risk pregnancy in women with pregestational, type 1, or type 2 diabetes mellitus remain challenging, depending on a comprehensive and multidisciplinary team approach and extensive preconception counseling.

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Section: Pregnancy and Diabetic Retinopathymentioning

confidence: 87%

Microvascular complications and the diabetic pregnancy

Bond

Umans²

2006

Curr Diab Rep

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“…For example, in diabetic retinopathy, frequent bleeding from newly formed vessels in the vitreous cavity results in impaired vision and loss of vitreous integrity. Retina-and eye-derived growth factors, implicated as causative factors in the pathology (Hill et al, 1983) have been identified as acidic and basic FGFs (Baird et al, 1985). Basic FGF has been shown to be an autocrine growth factor for human melanoma (Halaban et al, 1988).…”

Section: Time (Hours)mentioning

confidence: 98%

The basic fibroblast growth factor‐saporin mitotoxin acts through the basic fibroblast growth factor receptor

Lappi

Maher

Martineau

et al. 1991

Journal Cellular Physiology

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We have confirmed the hypothesis that a mitotoxin resulting from the conjugation of basic fibroblast growth factor and saporin exerts its cytotoxic effect through specific interaction with the basic fibroblast growth factor (FGF) receptor. Accordingly, the mitotoxin stimulates tyrosine phosphorylation of the 90 kD substrate that characterizes the initial cellular response to basic FGF. Cross-linking experiments show that radio-labeled basic fibroblast growth factor-saporin (FGF-SAP) binds to the receptor. Suramin, an inhibitor of growth factor receptor binding, inhibits the cytotoxicity of basic FGF-SAP. In a study of 4 different cell types, there is a decrease in the ED50 of the mitotoxin as the receptor number per cell increases. We have verified the cytotoxicity of the mitotoxin in 3 different assay systems. As expected, it is effective in the inhibition of protein synthesis and DNA synthesis, as well as of cell count. Binding of basic FGF-SAP which will result in cytotoxicity occurs very rapidly; 5 minutes of incubation of 10 nM basic FGF-SAP with cells results in 80% inhibition of cell count. The in vitro data indicate that the basic FGF-SAP is a receptor specific and potent suicide antagonist of basic FGF. Its potential as an anti-FGF for therapeutic and research uses in vivo is discussed.

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“…It is believed that neovascularization exhibited in the advanced diabetic proliferative retinopathy is a hazardous lesion which leads to permanent blindness (Blankenship 1981). The neovascular response is highly possibly mediated by some angiogenesis factors produced in the retina (Chen and Chen 1980;Glaser et al 1980) and vitreous body Hill et al 1983). These factors are present in the eye not only in diabetic patients with proliferative retinopathy but also in normal animals and human.…”

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confidence: 99%