Angiogenic Effect of Leptin in the Quail Chorioallantoic Membrane

Výboh, Pavel; Zeman, Michal; Bilčı́k, Boris; Šárniková, B.; Košťál, Ľubor

doi:10.2754/avb201079010013

Cited by 11 publications

(5 citation statements)

References 23 publications

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“…But no reports have yielded conclusion results. Reports have also suggested that this dual function of leptin depends on multiple factors such as dose, time of incubation, mode of administration and to certain extent the type of species [ 3 , 4 , 8 – 10 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane

Manjunathan

Ragunathan

2015

biol res

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BackgroundLeptin, the cytokine produced by white adipose tissue is known to regulate food energy homeostasis through its hypothalamic receptor. In vitro studies have demonstrated that leptin plays a major role in angiogenesis through binding to the receptor Ob-R present on ECs by stimulating and initiating new capillary like structures from ECs. Various in vivo studies indicate that leptin has diverse effect on angiogenesis. A few reports have showed that leptin exerts pro angiogenic effects while some suggested that it has antiangiogenic potential. It is theoretically highly important to understand the effect of leptin on angiogenesis to use as a therapeutic molecule in various angiogenesis related pathological conditions. Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h using gelatin sponge. Images where taken after every 24 h of incubation and analysed with Angioguant software. The treated area was observed under microscope and histological evaluation was performed for the same. Tissue thickness was calculated morphometrically from haematoxylin and eosin stained cross sections. Reverse transcriptase PCR and immunohistochemistry were also performed to study the gene and protein level expression of angiogenic molecules.ResultsHRL has the ability to induce new vessel formation at the treated area and growth of the newly formed vessels and cellular morphological changes occur in a dose dependent manner. Increase in the tissue thickness at the treated area is suggestive of initiation of new capillary like structures. Elevated mRNA and protein level expression of VEGF165 and MMP2 along with the activation of ECs as demonstrated by the presence of CD34 expression supports the neovascularization potential of HRL.ConclusionAngiogenic potential of HRL depends on the concentration and time of incubation and is involved in the activation of ECs along with the major interaction of VEGF 165 and MMP2. It is also observed that 3 μg of HRL exhibits maximum angiogenic potential at 72 h of incubation. Thus our data suggest that dose dependent angiogenic potential HRL could provide a novel role in angiogenic dependent therapeutics such as ischemia and wound healing conditions.

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Section: Discussionmentioning

confidence: 99%

In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane

Manjunathan

Ragunathan

2015

biol res

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“…For PDT preclinical studies, the chick CAM has been used as an in vivo model, in which photosensitizers were administered mainly intravenously or topically [5,17,20,21]. Along the wellestablished chick CAM model, the CAM of Japanese quail (Coturnix japonica) has also been used in drug testing [22][23][24]. Results obtained in both chicken and quail CAM assays are very similar, but there are some advantages in the use of the quail CAM model [25,26].…”

Section: Introductionmentioning

confidence: 99%

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

et al. 2014

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Photosensitizing properties of hypericin are well known, and the chicken chorioallantoic membrane has previously been used to test photodynamic effects of hypericin and other substances. In our study the photodynamic effect of hypericin in the ex ovo quail chorioallantoic membrane model was evaluated. Steady-state and time-resolved fluorescence spectroscopy of hypericin solution in PEG-400 and its mixture in PBS was performed to assess and characterize the process of aggregation and disaggregation of hypericin during the drug formulation preparation. A therapeutical formulation (2 µg/g of embryo weight) was topically applied on CAM into the silicone ring. Hypericin was excited by diode laser with wavelength 405 nm, fluence rate 140 mW/cm2, and fluence 16.8 J/cm2. Hypericin in 100% PEG-400 exhibits typical fluorescence spectra with a maximum of about 600 nm, while hypericin 10% PEG-400 formulation exhibits almost no fluorescence. Time resolved spectra analysis showed fluorescence decay of hypericin in 100% PEG-400 solution with a mean lifetime of 5.1 ns and in 10% PEG 4.1 ns. Damage of quail chorioallantoic membrane vasculature after photodynamic therapy ranged from hemorrhage and vanishing of capillary vessels to thrombosis, lysis, and hemorrhage of larger vessels.The presented findings suggest that quail embryos can be used as a suitable model to test the effect of hypericin and other photodynamic compounds.

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“…These two processes have been analyzed for fractal geometry in our study, using fractal dimension as a statistical descriptor of the space‐filling pattern and density [Parsons‐Wingerter et al, 2000]. This parameter has been used to monitor the action of molecules that increase or inhibit vascular development [Parsons‐Wingerter et al, 2006; Výboh et al, 2010]. Here, the fractal dimension of the YSM vascular network was the parameter used to show that 1 mT and 60 Hz MF inhibited the vascular process when quail eggs were exposed for 6–9 h/day for an incubation period between 48 and 72 h. The inhibition of vascular development is related to lower fractal dimension values, exhibited by Groups 2 and 3 (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Fractal analysis of extra‐embryonic vascularization in Japanese quail embryos exposed to extremely low frequency magnetic fields

Costa

Jimenez

Barbosa

et al. 2012

Bioelectromagnetics

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Magnetic fields (MF) can alter the dynamic behavior of vascular tissue and may have a stimulatory or inhibitory effect on blood vessel growth. Fractal geometry has been used in several studies as a tool to describe the development of blood vascular networks. Due to its self-similarity, irregularity, fractional dimension, and dependence on the scale of vessel dimensions, vascular networks can be taken as fractal objects. In this work, we calculated the fractal dimension by the methods of box counting (D(bc)) and information dimension (D(inf)) to evaluate the development of blood vessels of the yolk sac membrane (YSM) from quail embryos exposed to MF with a magnetic flux density of 1 mT and a frequency of 60 Hz. The obtained results showed that when the MF was applied to embryos aged between 48 and 72 h, in sessions of 2 h (6 h/day) and 3 h (9 h/day) with exposure intervals between 6 and 5 h, respectively, blood vascular formation was inhibited. Exposure sessions shorter than 2 h or longer than 3 h had no observable change on the vascular process. In contrast, the magnetic field had no observable change on the YSM vascular network for embryos aged between 72 and 96 h, irrespective of the exposure time. In conclusion, these results show a "window effect" regarding exposure time.

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Angiogenic Effect of Leptin in the Quail Chorioallantoic Membrane

Cited by 11 publications

References 23 publications

In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane

In ovo administration of human recombinant leptin shows dose dependent angiogenic effect on chicken chorioallantoic membrane

Photodynamic Effect of Hypericin after Topical Application in the Ex Ovo Quail Chorioallantoic Membrane Model

Fractal analysis of extra‐embryonic vascularization in Japanese quail embryos exposed to extremely low frequency magnetic fields

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