Angiogenic Biomaterials to Promote Tissue Vascularization and Integration

Wells, Laura; Valic, Michael; Lisovsky, Alexandra; Sefton, Michael V.

doi:10.1002/ijch.201300053

Cited by 13 publications

(6 citation statements)

References 64 publications

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“…VE-cadherin was expressed on HUVEC incubated on the films for 96 hours albeit with lower amounts with MAA copolymers in comparison controls. 2 The alamarBlue® assay was used to assess viability of the cells on the films over time. The alamarBlue absorbance ratio for dTHP1 cells (Fig.…”

Section: Cell Viability On Filmsmentioning

confidence: 99%

“…1 Designing biomaterials that promote vascularization can use many different approachesbased on biomolecule delivery or the control of polymer properties such as porosity. 2 Polymer beads containing 45% methacrylic acid-co-methyl methacrylate (MAA beads) promoted functional vascularization in wound beds and accelerated full thickness cutaneous healing of wounds in diabetic mice 3 and skin grafts in rats, 4 without the addition of growth factors. In vitro, macrophage-like cells (dTHP1) grown with MAA beads (versus controls) had decreased osteopontin gene expression and increased IL6, IL1β and TNFα gene expression with no significant changes in typical angiogenesis-associated genes (FGF2, SDF1α, HIF1α, PDGFβ, VEGF, etc.…”

Section: Introductionmentioning

confidence: 99%

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The effect of methacrylic acid in smooth coatings on dTHP1 and HUVEC gene expression

Wells

Sefton

2014

Biomater. Sci.

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Section: Cell Viability On Filmsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of methacrylic acid in smooth coatings on dTHP1 and HUVEC gene expression

Wells

Sefton

2014

Biomater. Sci.

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“…Indeed, many biomaterials have been developed to promote angiogenesis. These include hydrogels, topical creams, growth factors, small molecules and polymers. , Yet, there is still interest in developing new options for successful treatment of ischemic and chronic wounds.…”

Section: Introductionmentioning

confidence: 99%

A Heparin-Mimicking Block Copolymer Both Stabilizes and Increases the Activity of Fibroblast Growth Factor 2 (FGF2)

et al. 2016

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Fibroblast growth factor 2 (FGF2) is a protein involved in cellular functions in applications such as wound healing and tissue regeneration. Stabilization of this protein is important for its use as a therapeutic since the native protein is unstable during storage and delivery. Additionally, the ability to increase the activity of FGF2 is important for its application, particularly in chronic wound healing and the treatment of various ischemic conditions. Here we report a heparin mimicking block copolymer, poly(styrenesulfonate-co-poly(ethylene glycol) methyl ether methacrylate)-b-vinyl sulfonate) (p(SS-co-PEGMA)-b-VS, that contains a segment that enhances the stability of FGF2 and one that binds to the FGF2 receptor. The FGF2 conjugate retained activity after exposure to refrigeration (4 °C) and room temperature (23 °C) for 7 days, while unmodified FGF2 was inactive after these standard storage conditions. A cell study performed with a cell line lacking native heparan sulfate proteoglycans indicated that the conjugated block copolymer facilitated binding of FGF2 to its receptor similar to the addition of heparin to FGF2. A receptor-based enzyme-linked immunosorbant assay (ELISA) confirmed the results. The conjugate also increased the migration of endothelial cells by 80% compared to FGF2 alone. Additionally, the FGF2-p(SS-co-PEGMA)-b-VS stimulated endothelial cell sprouting 250% better than FGF2 at low concentration. These data verify that this rationally designed protein-block copolymer conjugate enhances receptor binding, cellular processes such as migration and tube-like formation, and stability, and suggest that it may be useful for applications in biomaterials, tissue regeneration, and wound healing.

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“…However, IL‐8 fulfills important functions in angiogenesis, [ 45 ] which is an important feature of healing and might be beneficial for biomaterial integration. [ 46 ]…”

Section: Discussionmentioning

confidence: 99%

Translation of Collagen Ultrastructure to Biomaterial Fabrication for Material‐Independent but Highly Efficient Topographic Immunomodulation

et al. 2021

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Supplement‐free induction of cellular differentiation and polarization solely through the topography of materials is an auspicious strategy but has so far significantly lagged behind the efficiency and intensity of media‐supplementation‐based protocols. Consistent with the idea that 3D structural motifs in the extracellular matrix possess immunomodulatory capacity as part of the natural healing process, it is found in this study that human‐monocyte‐derived macrophages show a strong M2a‐like prohealing polarization when cultured on type I rat‐tail collagen fibers but not on collagen I films. Therefore, it is hypothesized that highly aligned nanofibrils also of synthetic polymers, if packed into larger bundles in 3D topographical biomimetic similarity to native collagen I, would induce a localized macrophage polarization. For the automated fabrication of such bundles in a 3D printing manner, the strategy of “melt electrofibrillation” is pioneered by the integration of flow‐directed polymer phase separation into melt electrowriting and subsequent selective dissolution of the matrix polymer postprocessing. This process yields nanofiber bundles with a remarkable structural similarity to native collagen I fibers, particularly for medical‐grade poly(ε‐caprolactone). These biomimetic fibrillar structures indeed induce a pronounced elongation of human‐monocyte‐derived macrophages and unprecedentedly trigger their M2‐like polarization similar in efficacy as interleukin‐4 treatment.

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Angiogenic Biomaterials to Promote Tissue Vascularization and Integration

Cited by 13 publications

References 64 publications

The effect of methacrylic acid in smooth coatings on dTHP1 and HUVEC gene expression

The effect of methacrylic acid in smooth coatings on dTHP1 and HUVEC gene expression

A Heparin-Mimicking Block Copolymer Both Stabilizes and Increases the Activity of Fibroblast Growth Factor 2 (FGF2)

Translation of Collagen Ultrastructure to Biomaterial Fabrication for Material‐Independent but Highly Efficient Topographic Immunomodulation

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