Angiogenesis‐related gene expression profile in clinical cases of canine cancer

Abstract

The balance between pro‐ and anti‐angiogenic signalling is tightly regulated in normal tissues to maintain the functions of the vasculature. In contrast, the overproduction of angiogenic factors and enhanced angiogenesis are frequently observed in several types of tumours. Although there have been many reports on the correlation between tumour progression and angiogenesis in humans, little is known about tumour angiogenesis in canines. Hence, we attempted to clarify whether angiogenesis contributes to tumour progression in canines as well as humans. In this study, we investigated the expression of several angiogenesis‐related genes, including CD 34 , VEGF ‐A , VEGFR ‐1 , VEGFR ‐2 , Ang‐1 , Ang‐2 , Tie1, and Tie2 , in 66 canine tumour tissues and in the normal tissues surrounding the tumours by quantitative real‐time PCR analysis. Our comparative analysis between canine tumour tissues and normal tissues revealed that several angiogenesis‐related genes, such as vascular endothelial growth factor ( VEGF ) and VEGF ‐receptor genes, were significantly upregulated in canine tumour tissues when compared to the normal tissues. We also found that the angiopoietin ( Ang ) ‐1/Ang‐2 gene expression ratio was lower in canine tumour tissues than in the normal tissues, suggesting less association between vascular endothelial cells and perivascular cells in the canine tumour tissues. Taken together, our results suggest that several angiogenesis‐related genes may contribute to the malignant progression of canine tumours via tumour angiogenesis.

An Update on Molecular Pathways Regulating Vasculogenic Mimicry in Human Osteosarcoma and Their Role in Canine Oncology

“…As far as VEGF/VEGFR axis in veterinary oncology is concerned, VEGF family members were identified in several canine cancers ( 76 ), as well as OSA tissue, serum, and cultured cells ( 77 – 79 ). A relation between VM and VERGFR was found in D17 canine OSA cells cultured on type I collagen where malignant cancer cells with endothelial morphology express VEGFR1 ( 14 ).…”