Angiogenesis-promoted bone repair with silicate-shelled hydrogel fiber scaffolds

Dashnyam, Khandmaa; Buitrago, Jennifer O.; Bold, Tsendmaa; Mandakhbayar, Nandin; Pérez, Román A.; Knowles, Jonathan C.; Lee, Jung Hwan; Kim, Hae‐Won

doi:10.1039/c9bm01103j

Cited by 43 publications

(26 citation statements)

References 52 publications

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“…Neoangiogenesis plays an important role in tissue regeneration by providing adequate nutrition. Silicon has also been considered a potent element that promotes angiogenesis at therapeutic doses [ 35 ]. Release of silicic acid is considered to be the major factor in promoting osteogenesis of bioceramic materials [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration

Jiao

Wan

et al. 2022

Bioactive Materials

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Sensory nerves promote osteogenesis through the release of neuropeptides. However, the potential application and mechanism in which sensory nerves promote healing of bone defects in the presence of biomaterials remain elusive. The present study identified that new bone formation was more abundantly produced after implantation of silicified collagen scaffolds into defects created in the distal femur of rats. The wound sites were accompanied by extensive nerve innervation and angiogenesis. Sensory nerve dysfunction by capsaicin injection resulted in significant inhibition of silicon-induced osteogenesis in the aforementioned rodent model. Application of extracellular silicon in vitro induced axon outgrowth and increased expression of semaphorin 3 A (Sema3A) and semaphorin 4D (Sema4D) in the dorsal root ganglion (DRG), as detected by the upregulation of signaling molecules. Culture medium derived from silicon-stimulated DRG cells promoted proliferation and differentiation of bone marrow mesenchymal stem cells and endothelial progenitor cells. These effects were inhibited by the use of Sema3A neutralizing antibodies but not by Sema4D neutralizing antibodies. Knockdown of Sema3A in DRG blocked silicon-induced osteogenesis and angiogenesis almost completely in a femoral defect rat model, whereas overexpression of Sema3A promoted the silicon-induced phenomena. Activation of “mechanistic target of rapamycin” (mTOR) pathway and increase of Sema3A production were identified in the DRG of rats that were implanted with silicified collagen scaffolds. These findings support the role of silicon in inducing Sema3A production by sensory nerves, which, in turn, stimulates osteogenesis and angiogenesis. Taken together, silicon has therapeutic potential in orthopedic rehabilitation.

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Section: Discussionmentioning

confidence: 99%

Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration

Jiao

Wan

et al. 2022

Bioactive Materials

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“…When sealers or their toxic components in extraction are exposed to the surrounding tissues outside the root canal (periapical lesion) [ 17 , 18 ], inflammation of the periapical tissue can be affected and delayed wound healing can result [ 17 , 19 , 20 ]. Calcium silicate powders are known to have excellent bioactivity, and possibly released components, such as calcium and silicate ions, have been known to accelerate the healing process, along with additional antibacterial effects by alkaline pH [ 21 , 22 , 23 , 24 ]. In particular, calcium and silicate ions can accelerate osteoinduction and angiogenesis, respectively, all of which are major biological properties that boost the periapical healing process [ 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Kim

Lee

et al. 2020

Nanomaterials

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Calcium silicate-based bioactive glass has received significant attention for use in various biomedical applications due to its excellent bioactivity and biocompatibility. However, the bioactivity of calcium silicate nanoparticle-incorporated bioactive dental sealer is not much explored. Herein, three commercially available bioactive root canal sealers (Endoseal MTA (EDS), Well-Root ST (WST), and Nishika Canal Sealer BG (NBG)) were compared with a resin-based control sealer (AH Plus (AHP)) in terms of physical, chemical, and biological properties. EDS and NBG showed 200 to 400 nm and 100 to 200 nm nanoparticle incorporation in the SEM image, respectively, and WST and NBG showed mineral deposition in Hank’s balanced salt solution after 28 days. The flowability and film thickness of all products met the ISO 3107 standard. Water contact angle, linear dimensional changes, and calcium and silicate ion release were significantly different among groups. All bioactive root canal sealers released calcium ions, while NBG released ~10 times more silicon ions than the other bioactive root canal sealers. Under the cytocompatible extraction range, NBG showed prominent cytocompatibility, osteogenecity, and angiogenecity compared to other sealers in vitro. These results indicate that calcium silicate nanoparticle incorporation in dental sealers could be a potential strategy for dental periapical tissue regeneration.

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“…Bone defect repair is a complex and multifactorial interactive process ( Mavrogenis et al, 2009 ; Dashnyam et al, 2019 ). Bone trauma and bone defects lead to local vascular rupture followed by bleeding, which leads to tissue ischemia, hypoxia, and acidosis ( Ma et al, 2020 ; Wu et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%

Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium

Wang

Zhang

et al. 2021

Front. Physiol.

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Nowadays, the bone osseointegration in different environments is comparable, but the mechanism is unclear. This study aimed to investigate the osseointegration of different bioactive titanium surfaces under normoxic or high-altitude hypoxic environments. Titanium implants were subjected to one of two surface treatments: (1) sanding, blasting, and acid etching to obtain a rough surface, or (2) extensive polishing to obtain a smooth surface. Changes in the morphology, proliferation, and protein expression of osteoblasts on the rough and smooth surfaces were examined, and bone formation was studied through western blotting and animal-based experiments. Our findings found that a hypoxic environment and rough titanium implant surface promoted the osteogenic differentiation of osteoblasts and activated the JAK1/STAT1/HIF-1α pathway in vitro. The animal study revealed that following implant insertion in tibia of rabbit, bone repair at high altitudes was slower than that at low altitudes (i.e., in plains) after 2weeks; however, bone formation did not differ significantly after 4weeks. The results of our study showed that: (1) The altitude hypoxia environment would affect the early osseointegration of titanium implants while titanium implants with rough surfaces can mitigate the effects of this hypoxic environment on osseointegration, (2) the mechanism may be related to the activation of JAK1/STAT1/HIF-1α pathway, and (3) our results suggest the osteogenesis of titanium implants, such as oral implants, is closely related to the oxygen environment. Clinical doctors, especially dentists, should pay attention to the influence of hypoxia on early osseointegration in patients with high altitude. For example, it is better to choose an implant system with rough implant surface in the oral cavity of patients with tooth loss at high altitude.

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Angiogenesis-promoted bone repair with silicate-shelled hydrogel fiber scaffolds

Abstract: The silicate-shelled alginate hydrogel fiber scaffold is highly effective for promoting ion-induced angiogenesis and bone bioactivity, ultimately useful for the repair and regeneration of hard tissues.

Cited by 43 publications

References 52 publications

Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration

Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration

Physical Properties and Biofunctionalities of Bioactive Root Canal Sealers In Vitro

Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium

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