Angiogenesis and lymphangiogenesis in inflammatory skin disorders

Varricchi, Gilda; Granata, Francescopaolo; Loffredo, Stefania; Genovese, Arturo; Marone, Gianni

doi:10.1016/j.jaad.2015.03.041

Cited by 143 publications

(116 citation statements)

References 142 publications

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“…IL-17A has been demonstrated to participate in the pathological angiogenesis in rheumatoid arthritis, psoriasis and tumors (Numasaki et al 2005;Kim et al 2013;Varricchi et al 2015). In addition, IL-17A/F is expressed at the lesion of psoriasis, chronic obstructive pulmonary disease and rheumatoid arthritis (Chang et al 2014;Kakeda et al 2014;Sarkar et al 2014).…”

Section: Dmentioning

confidence: 99%

A Heterodimeric Cytokine, Consisting of IL-17A and IL-17F, Promotes Migration and Capillary-Like Tube Formation of Human Vascular Endothelial Cells

Numasaki

Tsukamoto

Tomioka

et al. 2016

Tohoku J. Exp. Med.

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Section: Dmentioning

confidence: 99%

A Heterodimeric Cytokine, Consisting of IL-17A and IL-17F, Promotes Migration and Capillary-Like Tube Formation of Human Vascular Endothelial Cells

Numasaki

Tsukamoto

Tomioka

et al. 2016

Tohoku J. Exp. Med.

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“…Many highly inflammatory chronic skin wounds have altered angiogenesis. 49 Proper angiogenesis is a crucial step toward complete wound healing. 50 Therefore, even with no significant change in dermal thickness between control and acellular hydrogels, the increased vasculature in the wounds treated with acellular hydrogels may indicate further advanced wound healing, possibly related to the decreased inflammation seen 14 days post skin biopsy.…”

mentioning

confidence: 99%

Cellularized Bilayer Pullulan-Gelatin Hydrogel for Skin Regeneration

Nicholas

Jeschke

Amini-Nik

2016

Tissue Engineering Part A

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Skin substitutes significantly reduce the morbidity and mortality of patients with burn injuries and chronic wounds. However, current skin substitutes have disadvantages related to high costs and inadequate skin regeneration due to highly inflammatory wounds. Thus, new skin substitutes are needed. By combining two polymers, pullulan, an inexpensive polysaccharide with antioxidant properties, and gelatin, a derivative of collagen with high water absorbency, we created a novel inexpensive hydrogel-named PG-1 for ''pullulan-gelatin first generation hydrogel''-suitable for skin substitutes. After incorporating human fibroblasts and keratinocytes onto PG-1 using centrifugation over 5 days, we created a cellularized bilayer skin substitute. Cellularized PG-1 was compared to acellular PG-1 and no hydrogel (control) in vivo in a mouse excisional skin biopsy model using newly developed dome inserts to house the skin substitutes and prevent mouse skin contraction during wound healing. PG-1 had an average pore size of 61.69 mm with an ideal elastic modulus, swelling behavior, and biodegradability for use as a hydrogel for skin substitutes. Excellent skin cell viability, proliferation, differentiation, and morphology were visualized through live/dead assays, 5-bromo-2¢-deoxyuridine proliferation assays, and confocal microscopy. Trichrome and immunohistochemical staining of excisional wounds treated with the cellularized skin substitute revealed thicker newly formed skin with a higher proportion of actively proliferating cells and incorporation of human cells compared to acellular PG-1 or control. Excisional wounds treated with acellular or cellularized hydrogels showed significantly less macrophage infiltration and increased angiogenesis 14 days post skin biopsy compared to control. These results show that PG-1 has ideal mechanical characteristics and allows ideal cellular characteristics. In vivo evidence suggests that cellularized PG-1 promotes skin regeneration and may help promote wound healing in highly inflammatory wounds, such as burns and chronic wounds.

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“…VEGF is an angiogenic factor and several anti-VEGF therapies are being used in the treatment of diseases characterised by abnormal blood vessel formation [25]. VEGF is important in regulation of the immunological response and has activity in immediate and delayed type hypersensitivity [26] as well as some acute inflammatory skin disorders like urticaria and UV-B induced damage [27]. TNF, although discovered as a result of its antitumor activity in the beginning, has now been found having functions in tumor initiation, progression, and metastasis [28].…”

Section: Gene Expression Analysis (Real-time Qpcr)mentioning

confidence: 99%

Evaluating Antiinflammatory and Antiandrogenic Effects of Garden Cress (Lepidium sativum L.) in HaCaT cells

Türkoğlu¹,

Kılıç²,

Pekmezci³

et al. 2018

Rec.Nat.Prod.

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Lepidium sativum L. (Brassicaceae) (Ls), garden cress, is an edible herb that is botanically related to watercress and mustard, sharing their peppery, tangy flavor and aroma. The various parts of the plant have been traditionally used for many respiratory, rheumatologic, cardiovasculary, metabolic, and gastrointestinal disorders. Several studies were performed showing its antiinflammatory, anticarcinogenic, antiproliferative as well as tissue protective effects. In all of these studies the positive effects of Ls were concluded as the result of its antioxidant features due to the polyphenols and organosulphur compounds. In this study, we investigated the molecular basis of antiinflammatory, antiproliferative, antimitotic and antiandrogenic effects of Ls leaf extract in HaCaT cells. The gene expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factoralpha (TNF-α), interleukin 1-alpha (IL-1α), and 5 alpha-reductase type II (SRD5A2) were studied. Gene expressions were determined by RT-qPCR analysis. Fold change results of the gene expression analyses showed that the plant extract caused statistically significant downregulation of VEGF (0.2167, p<0.0001), p = 0.0262), and SRD5A2 (0.0567, p<0.0001). The fold change for IL-1α was 0.6900 and this result was not statistically significant (p = 0.1522). These preliminary results may partially explain the clinical success of Ls in the traditional medicine. Considering these results, topical application of Lepidium sativum extract may be beneficial for inflammatory, androgen dependent disorders of the skin.

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Angiogenesis and lymphangiogenesis in inflammatory skin disorders

Cited by 143 publications

References 142 publications

A Heterodimeric Cytokine, Consisting of IL-17A and IL-17F, Promotes Migration and Capillary-Like Tube Formation of Human Vascular Endothelial Cells

A Heterodimeric Cytokine, Consisting of IL-17A and IL-17F, Promotes Migration and Capillary-Like Tube Formation of Human Vascular Endothelial Cells

Cellularized Bilayer Pullulan-Gelatin Hydrogel for Skin Regeneration

Evaluating Antiinflammatory and Antiandrogenic Effects of Garden Cress (Lepidium sativum L.) in HaCaT cells

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