Angiogenesis and Functional Vessel Formation Induced by Interstitial Flow and Vascular Endothelial Growth Factor Using a Microfluidic Chip

Liu, Yufang; Li, Jiao; Zhou, Jiasheng; Li, Xue; Li, Huibing; Lu, Yao; Lin, Bingcheng; Li, Xiaojie; Liu, Tingjiao

doi:10.3390/mi13020225

Cited by 18 publications

(21 citation statements)

References 42 publications

(43 reference statements)

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“…However, it remains unclear what is hidden under the terms “low” and “high”. Some suggest the use of VEGF solution with a concentration of 2.5–5 ng/mL [ 26 ] while others with a concentration of 50 ng/mL [ 22 ] or even 100–500 ng/mL [ 23 ]. As available data are divergent, we have examined the influence of VEGF with concentrations of 5, 25, 50, and 100 ng/mL on the course of angiogenesis under coherent conditions.…”

Section: Discussionmentioning

confidence: 99%

“…A key agent that determines the initiation of angiogenesis is Vascular Endothelial Growth Factor (VEGF) [ 20 , 21 ]. It has already been successfully used to initiate angiogenesis in microfluidics [ 22 , 23 , 24 , 25 ]. Its concentration can be regulated directly or indirectly.…”

Section: Introductionmentioning

confidence: 99%

“…However, what is hidden under the term “low” remains unclear. Some suggest the use of VEGF solution with a concentration starting from 2.5–5 ng/mL [ 26 ], while others with a concentration of 50 ng/mL [ 22 ] or even 100–500 ng/mL [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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Angiogenesis is the development of new blood vessels from the existing vasculature. Its malfunction leads to the development of cancers and cardiovascular diseases qualified by the WHO as a leading cause of death worldwide. A better understanding of mechanisms regulating physiological and pathological angiogenesis will potentially contribute to developing more effective treatments for those urgent issues. Therefore, the main goal of the following study was to design and manufacture an angiogenesis-on-a-chip microplatform, including cylindrical microvessels created by Viscous Finger Patterning (VFP) technique and seeded with HUVECs. While optimizing the VFP procedure, we have observed that lumen’s diameter decreases with a diminution of the droplet’s volume. The influence of Vascular Endothelial Growth Factor (VEGF) with a concentration of 5, 25, 50, and 100 ng/mL on the migration of HUVECs was assessed. VEGF’s solution with concentrations varying from 5 to 50 ng/mL reveals high angiogenic potential. The spatial arrangement of cells and their morphology were visualized by fluorescence and confocal microscopy. Migration of HUVECs toward loaded angiogenic stimuli has been initiated after overnight incubation. This research is the basis for developing more complex vascularized multi-organ-on-a-chip microsystems that could potentially be used for drug screening.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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“…Thus, they allow for better replication of several parameters that influence in vivo NP delivery. Microfluidic devices already demonstrated their potential in the study of angiogenesis, metastasis, isolation, drug screening, and NP penetration and uptake . The delivery of NPs to the tumor bed is a multistep process that requires overcoming several challenges, such as vessel extravasation, target specificity, tumoral heterogeneity, and cellular internalization for the delivery of the therapeutic agents .…”

Section: Microfluidic Devicesmentioning

confidence: 99%

Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation

Gimondi,

Ferreira,

Reis

et al. 2023

ACS Nano

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The use of nanoparticles (NPs) in nanomedicine holds great promise for the treatment of diseases for which conventional therapies present serious limitations. Additionally, NPs can drastically improve early diagnosis and follow-up of many disorders. However, to harness their full capabilities, they must be precisely designed, produced, and tested in relevant models. Microfluidic systems can simulate dynamic fluid flows, gradients, specific microenvironments, and multiorgan complexes, providing an efficient and cost-effective approach for both NPs synthesis and screening. Microfluidic technologies allow for the synthesis of NPs under controlled conditions, enhancing batch-to-batch reproducibility. Moreover, due to the versatility of microfluidic devices, it is possible to generate and customize endless platforms for rapid and efficient in vitro and in vivo screening of NPs’ performance. Indeed, microfluidic devices show great potential as advanced systems for small organism manipulation and immobilization. In this review, first we summarize the major microfluidic platforms that allow for controlled NPs synthesis. Next, we will discuss the most innovative microfluidic platforms that enable mimicking in vitro environments as well as give insights into organism-on-a-chip and their promising application for NPs screening. We conclude this review with a critical assessment of the current challenges and possible future directions of microfluidic systems in NPs synthesis and screening to impact the field of nanomedicine.

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“…Alternatively, the vessel can also sprout from an adjacent vascular bed formed by vasculogenesis ( 27 ). Under the stimulation of interstitial flow or certain growth factor gradients, such as the VEGF gradient ( Figure 1D ), which can be introduced by supplementing exogenous growth factors or coculture with stromal cells or certain types of cancer cells, ECs are activated and start to proliferate and sprout vessels toward the direction of the stimulus ( 28 , 29 ). The direction of interstitial flow can also greatly affect angiogenic sprouting where interstitial flow in the direction opposite to vessel sprouting enhances angiogenesis while flow in the same direction suppresses vessel sprouting ( 27 ).…”

Section: Vascularization Strategiesmentioning

confidence: 99%

Recent advances in vascularized tumor-on-a-chip

Huang

2023

Front. Oncol.

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The vasculature plays a critical role in cancer progression and metastasis, representing a pivotal aspect in the creation of cancer models. In recent years, the emergence of organ-on-a-chip technology has proven to be a robust tool, capable of replicating in vivo conditions with exceptional spatiotemporal resolution, making it a significant asset in cancer research. This review delves into the latest developments in 3D microfluidic vascularized tumor models and their applications in vitro, focusing on heterotypic cellular interactions, the mechanisms of metastasis, and therapeutic screening. Additionally, the review examines the benefits and drawbacks of these models, as well as the future prospects for their advancement.

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Angiogenesis and Functional Vessel Formation Induced by Interstitial Flow and Vascular Endothelial Growth Factor Using a Microfluidic Chip

Cited by 18 publications

References 42 publications

Exploring Endothelial Expansion on a Chip

Exploring Endothelial Expansion on a Chip

Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation

Recent advances in vascularized tumor-on-a-chip

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