Angiogenesis

Warmke, Nele; Walker, Ameae M.; Cubbon, R

doi:10.1016/b978-0-12-809657-4.99736-9

Cited by 9 publications

(3 citation statements)

References 101 publications

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“…It mainly occurs during embryonic development, and it results in primary vascular plexus formation. The primary plexus grows into more complex networks throughout a process called angiogenesis (aka neovascularization) [ 1 ]. Under physiological conditions, angiogenesis is involved in multiple processes such as wound healing [ 2 ], placenta formation, growth of the endometrium [ 3 ], cyclical growth of hair and follicles [ 4 ], and the weight gain of trained skeletal muscles [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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Angiogenesis is the development of new blood vessels from the existing vasculature. Its malfunction leads to the development of cancers and cardiovascular diseases qualified by the WHO as a leading cause of death worldwide. A better understanding of mechanisms regulating physiological and pathological angiogenesis will potentially contribute to developing more effective treatments for those urgent issues. Therefore, the main goal of the following study was to design and manufacture an angiogenesis-on-a-chip microplatform, including cylindrical microvessels created by Viscous Finger Patterning (VFP) technique and seeded with HUVECs. While optimizing the VFP procedure, we have observed that lumen’s diameter decreases with a diminution of the droplet’s volume. The influence of Vascular Endothelial Growth Factor (VEGF) with a concentration of 5, 25, 50, and 100 ng/mL on the migration of HUVECs was assessed. VEGF’s solution with concentrations varying from 5 to 50 ng/mL reveals high angiogenic potential. The spatial arrangement of cells and their morphology were visualized by fluorescence and confocal microscopy. Migration of HUVECs toward loaded angiogenic stimuli has been initiated after overnight incubation. This research is the basis for developing more complex vascularized multi-organ-on-a-chip microsystems that could potentially be used for drug screening.

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Section: Introductionmentioning

confidence: 99%

Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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“…He hypothesised that tumour growth depends on angiogenesis to increase blood supply and proposed stopping the blood supply from inhibiting tumour growth, which subsequently initiated the field of research on the relationship between angiogenesis and diseases. Angiogenesis is the process of capillary sprouting from preexisting vasculature, and it is highly induced by hypoxia and other biological processes [ 5 , 7 ]. The mechanisms underlying angiogenesis can be divided into two types.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Mechanism of Traditional Chinese Medicines in Angiogenesis through Network Pharmacology and Data Mining

Yun

Dan

Liu

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Although traditional Chinese medicine is effective and safe for the treatment of angiogenesis, the in vivo intervention mechanism is diverse, complex, and largely unknown. Therefore, we aimed to explore the active ingredients of traditional Chinese medicine and their mechanisms of action against angiogenesis. Data on angiogenesis-related targets were collected from GeneCards, Therapeutic Target Database, Online Mendelian Inheritance in Man, DrugBank, and DisGeNET. These were matched to related molecular compounds and ingredients in the traditional Chinese medicine system pharmacology platform. The data were integrated and based on the condition of degree > 1, and relevant literature, target-compound, compound-medicine, and target-compound-medicine networks were constructed using Cytoscape. Molecular docking was used to predict the predominant binding combination of core targets and components. We obtained 79 targets for angiogenesis; 41 targets were matched to 3839 compounds, of which 110 compounds were selected owing to their high correlation with angiogenesis. Fifty-five combinations in the network were obtained by molecular docking, among which PTGS2-astragalin (−9.18 kcal/mol), KDR-astragalin (−7.94 kcal/mol), PTGS2-quercetin (−7.41 kcal/mol), and PTGS2-myricetin (−7.21 kcal/mol) were top. These results indicated that the selected potential core compounds have good binding activity with the core targets. Eighty new combinations were obtained from the network, and the top combinations based on affinity were KDR-beta-carotene (−10.13 kcal/mol), MMP9-beta-sitosterol (−8.04 kcal/mol), MMP9-astragalin (−7.82 kcal/mol), and MMP9-diosgenin (−7.51 kcal/mol). The core targets included PTGS2, KDR, VEGFA, and MMP9. The essential components identified were astragalin, kaempferol, myricetin, quercetin, and β-sitosterol. The crucial Chinese medicines identified included Polygoni Cuspidati Rhizoma et Radix, Morus alba Root Bark, and Forsythiae Fructus. By systematically analysing the ingredients of traditional Chinese medicine and their targets, it is possible to determine their potential mechanisms of action against pathological angiogenesis. Our study provides a basis for further research and the development of new therapeutics for angiogenesis.

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“…He hypothesised that tumour growth depends on angiogenesis to increase blood supply and proposed stopping the blood supply to inhibit tumour growth, which subsequently initiated the field of research on the relationship between angiogenesis and diseases. Angiogenesis is the process of capillary sprouting from pre-existing vasculature, and it is highly induced by hypoxia and other biological processes 5,7 . The mechanism of angiogenesis can be divided into two types.…”

mentioning

confidence: 99%

Investigation of the Mechanism of Traditional Chinese Medicines in Angiogenesis Through Network Pharmacology and Data Mining

Yun

Dan

Liu

et al. 2020

Preprint

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BackgroundAlthough traditional Chinese medicine is safe for the clinical treatment of angiogenesis, the in vivo intervention mechanism is diverse, complex, and largely unknown. Therefore, we aimed to explore the active ingredients of traditional Chinese medicine and their mechanisms for the treatment of angiogenesis.MethodsData on angiogenesis-related targets were collected from the GeneCards, Therapeutic Target Database, Online Mendelian Inheritance in Man, DrugBank, and DisGeNET databases. These were matched to related molecular compounds and ingredients in the traditional Chinese medicine system pharmacology platform. The data were integrated; based on the condition of Degree >1 and relevant literature, a target-compound network as well as compound-medicine and target-compound-medicine networks were constructed using Cytoscape. Molecular docking was used to predict the predominant binding combination of core targets and components.ResultsWe obtained a total of 79 targets for angiogenesis, and 41 targets were matched to 3839 compounds. Then, 110 compounds were selected owing to their high correlation with angiogenesis. Fifty-five combinations in the network were obtained by molecular docking, among which PTGS2-Astragalin (-9.18 kcal/mol), KDR-Astragalin (-7.94 kcal/mol), PTGS2-quercetin (-7.41 kcal/mol), and PTGS2-myricetin (-7.21 kcal/mol) were the top combinations. These results indicated that the selected potential core compounds may have good binding activity with the core targets. Eighty new combinations were obtained from the network, and the top combinations based on affinity were KDR-beta-carotene (-10.13 kcal/mol), MMP9-beta-Sitosterol (-8.04 kcal/mol), MMP9-Astragalin (-7.82 kcal/mol), and MMP9-Diosgenin (-7.51 kcal/mol). The core targets included PTGS2, KDR, VEGFA, and MMP9. The essential components identified were astragalin, kaempferol, myricetin, quercetin, and β-sitosterol. The crucial Chinese medicines identified included Polygoni Cuspidati Rhizoma et Radix, Morus alba Root Bark, and Forsythia Fructus.ConclusionsBy systematically analysing the essential ingredients of traditional Chinese medicine and their targets, it is possible to determine their potential mechanism of action in the treatment of pathological angiogenesis. Our study provides a basis for further research and development of new therapeutics for angiogenesis.

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Angiogenesis

Cited by 9 publications

References 101 publications

Exploring Endothelial Expansion on a Chip

Exploring Endothelial Expansion on a Chip

Investigation of the Mechanism of Traditional Chinese Medicines in Angiogenesis through Network Pharmacology and Data Mining

Investigation of the Mechanism of Traditional Chinese Medicines in Angiogenesis Through Network Pharmacology and Data Mining

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