Angiocrine Sphingosine-1-Phosphate Activation of S1PR2-YAP Signaling Axis in Alveolar Type II Cells Is Essential for Lung Repair

Chen, Qian; Rehman, Jalees; Chan, Manwai; Fu, Panfeng; Dudek, Steven M.; Natarajan, Viswanathan; Malik, Asrar B.; Liu, Yuru

doi:10.1016/j.celrep.2020.107828

Cited by 45 publications

(42 citation statements)

References 63 publications

(117 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…( Figure 1 B) Note: We use a rabbit anti pro-Sp-C antibody (see details in Key resources table ) and fluorescence conjugated anti-rabbit secondary antibodies in the immunofluorescence staining. See ( Chen et al., 2020 ) and ( Liu et al., 2015 ) for more details. Optional: The purity of isolated type II cells can also be determined by a PAP assay ( Dobbs, 1990 ).…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…However, the sex of the mice should always be taken into consideration when designing the experiments. For type II cell lineage labeling, we used SpC-CreER/ROSA-Tomato mice or SpC-CreER/mTmG mice ( Barkauskas et al., 2013 ; Rock et al., 2011 ) ( Chen et al., 2020 ). Note: The reason that we use AT2 lineage tracing mice is because by this it is easier for us to visualize AT2 in culture, especially 3D culture.…”

Section: Before You Beginmentioning

confidence: 99%

“…For type II cell lineage labeling, we used SpC-CreER/ROSA-Tomato mice or SpC-CreER/mTmG mice ( Barkauskas et al., 2013 ; Rock et al., 2011 ) ( Chen et al., 2020 ).…”

Section: Before You Beginmentioning

confidence: 99%

“…S1P or an inhibitor of S1P signaling is added in culture to modulate AT2 to AT1 transition. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) .…”

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) .…”

mentioning

confidence: 99%

See 4 more Smart Citations

Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation

Chen

Liu

2021

STAR Protocols

Self Cite

View full text Add to dashboard Cite

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

“…For type II cell lineage labeling, we used SpC-CreER/ROSA-Tomato mice or SpC-CreER/mTmG mice ( Barkauskas et al., 2013 ; Rock et al., 2011 ) ( Chen et al., 2020 ).…”

Section: Before You Beginmentioning

confidence: 99%

mentioning

confidence: 99%

“…For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) .…”

mentioning

confidence: 99%

See 3 more Smart Citations

Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation

Chen

Liu

2021

STAR Protocols

Self Cite

View full text Add to dashboard Cite

Activation of yes‐associated protein mediates sphingosine‐1‐phosphate–induced proliferation and migration of pulmonary artery smooth muscle cells and its potential mechanisms

Shi

Wang

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

The aims of the present study were to examine the molecular mechanisms underlying sphingosine-1-phosphate (S1P)-induced rat pulmonary artery smooth muscle cells (PASMCs) proliferation/migration and to determine the effect of yesassociated protein (YAP) activation on S1P-induced PASMCs proliferation/migration and its potential mechanisms. S1P induced YAP dephosphorylation and nuclear translocation, upregulated microRNA-130a/b (miR-130a/b) expression, reduced bone morphogenetic protein receptor 2 (BMPR2), and inhibitor of DNA binding 1(Id1) expression, and promoted PASMCs proliferation and migration. Pretreatment of cells with Rho-associated protein kinase (ROCK) inhibitor Y27632 suppressed S1P-induced YAP activation, miR-130a/b upregulation, BMPR2/Id1 downregulation, and PASMCs proliferation/migration. Knockdown of YAP using small interfering RNA also suppressed S1P-induced alterations of miR-130a/b, BMPR2, Id1, and PASMCs behavior. In addition, luciferase reporter assay indicated that miR-130a/b directly regulated BMPR2 expression in PASMCs. Inhibition of miR-130a/b functions by anti-miRNA oligonucleotides attenuated S1P-induced BMPR2/Id1 downregulation and the proliferation and migration of PASMCs. Taken together, our study indicates that S1P induces activation of YAP through ROCK signaling and subsequently increases miR-130a/b expression, which, in turn, downregulates BMPR2 and Id1 leading to PASMCs proliferation and migration.

show abstract

Lung regeneration: diverse cell types and the therapeutic potential

Chen,

Li,

et al. 2024

MedComm

View full text Add to dashboard Cite

Lung tissue has a certain regenerative ability and triggers repair procedures after injury. Under controllable conditions, lung tissue can restore normal structure and function. Disruptions in this process can lead to respiratory system failure and even death, causing substantial medical burden. The main types of respiratory diseases are chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), and acute respiratory distress syndrome (ARDS). Multiple cells, such as lung epithelial cells, endothelial cells, fibroblasts, and immune cells, are involved in regulating the repair process after lung injury. Although the mechanism that regulates the process of lung repair has not been fully elucidated, clinical trials targeting different cells and signaling pathways have achieved some therapeutic effects in different respiratory diseases. In this review, we provide an overview of the cell type involved in the process of lung regeneration and repair, research models, and summarize molecular mechanisms involved in the regulation of lung regeneration and fibrosis. Moreover, we discuss the current clinical trials of stem cell therapy and pharmacological strategies for COPD, IPF, and ARDS treatment. This review provides a reference for further research on the molecular and cellular mechanisms of lung regeneration, drug development, and clinical trials.

show abstract

Angiocrine Sphingosine-1-Phosphate Activation of S1PR2-YAP Signaling Axis in Alveolar Type II Cells Is Essential for Lung Repair

Abstract: Highlights d S1P is released from lung endothelial cells upon Pseudomonas aeruginosa injury d Angiocrine S1P promotes alveolar type II to type I cell transition d S1P regulates type II cell progenitor functions via a S1PR2-YAP signaling axis

Cited by 45 publications

References 63 publications

Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation

Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation

Activation of yes‐associated protein mediates sphingosine‐1‐phosphate–induced proliferation and migration of pulmonary artery smooth muscle cells and its potential mechanisms

Lung regeneration: diverse cell types and the therapeutic potential

Contact Info

Product

Resources

About