Angelica polysaccharide mitigates lipopolysaccharide-evoked inflammatory injury by regulating microRNA-10a in neuronal cell line HT22

Abstract

Background: Angelica polysaccharide (AP) is disengaged from the roots of Angelica sinensis. The extensive pathological activities of AP have been discovered in disparate diseases. Nevertheless, the impression of AP in epilepsy (EP) remains unaware. The research attempted to probe the impact of AP on lipopolysaccharide (LPS)-evoked inflammatory injury in HT22 cells. Methods: AP were exploited to stimulate HT22 cells, cell cytotoxicity was monitored by CCK-8 assay. LPS was utilized to administrate HT22 cells to evoke inflammatory injury, meanwhile the involvements of AP in cell proliferation, apoptosis and inflammatory cytokines productions were examined. MicroRNA-10a (miR-10a) inhibitor and its negative control were one by one transfected into HT22 cells, the effect of miR-10a inhibition on LPS-and AP-treated cells was determined. NF-jB and JAK2/STAT3 pathways were ultimately detected. Results: AP promoted cell proliferation, inhibited apoptosis and suppressed IL-1b, TNF-a and IL-6 productions in LPS-stimulated HT22 cells. Additionally, AP raised miR-10a expression in HT22 cells administration with LPS. These functions of AP in LPS-disposed cells were conversed by miR-10a suppression. Further, AP interdicted NF-jB and JAK2/STAT3 pathways via enhancing miR-10a. Conclusions: Data corroboarted that AP mitigated LPS-evoked inflammatory injury through repression of NF-jB and JAK2/STAT3 pathways by regulating miR-10a in HT22 cells.

Angelica Polysaccharide Attenuates LPS-Induced Inflammation Response of Primary Dairy Cow Claw Dermal Cells Via NF-κB and MAPK Signaling Pathways

“…Accumulating evidences have con rmed the anti-in ammatory effect of AP. For example, AP mitigated the in ammatory injury induced by LPS in PC-12 cell line [17] and neuronal cell line HT22 [18]. Another study also proved that AP alleviated the LPS-induced in ammatory injury of human keratinocytes HaCaT cells [19].…”

Evidence and potential mechanisms of traditional Chinese medicine for the treatment of psoriasis vulgaris: a systematic review and meta-analysis

“…Besides antioxidation, it might inhibit the growth of KCs via enhancing activation of cleaved caspase-3 and PARP and improve depression via activating the ERK-CREB-BDNF signaling pathway (34)(35)(36). Angelica Sinensis polysaccharide could attenuate inflammation by repression of NF-jB and JAK2/STAT3 pathways (24,37). Astilbin was found to improve differentiation in HaCaT KCs with suppression of KRT5 and KRT14 and induction of KRT1 and KRT10 (25,26,38).…”

“…A large number of clinical trials have confirmed that TCM can alleviate psoriatic symptoms (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). Moreover, the applications of advanced analysis and detection technology reveal some of the therapeutic mechanisms behind TCM's potency such as anti-angiogenesis, inducing KCs apoptosis and anti-inflammation (24)(25)(26).…”

The Bioactivities and Potential Clinical Values of Angelica Sinensis Polysaccharides

“…It also could attenuate inflammatory injury through up-regulating sirtuin 1(SIRT1) expression and activating the nuclear factor-erythroid 2-related factor (NrF2)/ heme oxygenase-1 (HO-1) pathway in HACaT cells induced by LPS. 54,55 The mechanism of anti-inflammatory activity is shown in Figure 4 and relevant pathways are summarized in Table 3.…”