Angelica Dahurica ethanolic extract improves impaired wound healing by activating angiogenesis in diabetes

Abstract

Abnormal angiogenesis plays an important role in impaired wound healing and development of chronic wounds in diabetes mellitus. Angelica dahurica radix is a common traditional Chinese medicine with wide spectrum medicinal effects. In this study, we analyzed the potential roles of Angelica dahurica ethanolic extract (ADEE) in correcting impaired angiogenesis and delayed wound healing in diabetes by using streptozotocin-induced diabetic rats. ADEE treatment accelerated diabetic wound healing through inducing angiogenesis and granulation tissue formation. The angiogenic property of ADEE was subsequently verified ex vivo using aortic ring assays. Furthermore, we investigated the in vitro angiogenic activity of ADEE and its underlying mechanisms using human umbilical vein endothelial cells. ADEE treatment induced HUVECs proliferation, migration, and tube formation, which are typical phenomena of angiogenesis, in dose-dependent manners. These effects were associated with activation of angiogenic signal modulators, including extracellular signal-regulated kinase 1/2 (ERK1/2), Akt, endothelial nitric oxide synthase (eNOS) as well as increased NO production, and independent of affecting VEGF expression. ADEE-induced angiogenic events were inhibited by the MEK inhibitor PD98059, the PI3K inhibitor Wortmannin, and the eNOS inhibitor L-NAME. Our findings highlight an angiogenic role of ADEE and its ability to protect against impaired wound healing, which may be developed as a promising therapy for impaired angiogenesis and delayed wound healing in diabetes.

Lupeol, a Dietary Triterpene, Enhances Wound Healing in Streptozotocin-Induced Hyperglycemic Rats with Modulatory Effects on Inflammation, Oxidative Stress, and Angiogenesis

“…The most common complication in patients with diabetes is an altered skin wound healing process, leading to complications such as Diabetic Foot Ulcers (DFUs), which has caused major worldwide morbidity due to various clinical and socioeconomic issues [3]. Several research studies have demonstrated that wound healing is delayed by hyperglycemia during DM [4–6].…”

Mining of simple sequence repeats (SSRs) loci and development of novel transferability-across EST-SSR markers from de novo transcriptome assembly of Angelica dahurica

“…Angelica dahurica [1, 2], which is known as ‘Baizhi’ in China, is a perennial dicotyledonous herb of the family Apiaceae . It originated in Taiwan and is widely grown in Korea, China, Japan, and Russia [3]. A .…”

Aster koraiensis extract improves impaired skin wound healing during hyperglycemia

“…In a previous study, we found that the AKE improved diabetic complications such as diabetic retinopathy 10 . Several studies have reported that skin wound healing is impaired and delayed in hyperglycemia 4, 5, 12. However, the effect of AKE on hyperglycemia-induced impaired wound healing has not been investigated and the mechanisms underlying these effects remain unknown.…”

“…In particular, delayed wound healing is one of the most serious diabetic complications 4 . Several research studies have demonstrated that wound healing is delayed by hyperglycemia during DM 5, 6, 7…”