Angelica dahurica attenuates melanogenesis in B16F0 cells by repressing Wnt/β-catenin signaling

Fang, Chien-Liang; Goswami, Debakshee; Kuo, Chia‐Hua; Day, Cecilia Hsuan; Lin, Mei; Ho, Tsung‐Jung; Yang, Liang-Yo; Hsieh, Dennis Jine‐Yuan; Lin, Tao; Huang, Chih‐Yang

doi:10.1007/s13273-022-00250-0

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…The root extract of A. dahurica was investigated on NK-1R and Wnt/β-catenin signaling, and evaluated against NK-1R on melanogenesis in B16F0 cells. The extract showed a potent reduction in Neurokinin-1 receptor and Wnt/β-catenin signaling activities via reducing the expression of β-catenin, MITF, LEF-1, TYR, TRP1, and TRP2, and enhancing the expression of GSK3β [144].…”

Section: Skin Whiteningmentioning

confidence: 98%

Phytochemical Constituents, Folk Medicinal Uses, and Biological Activities of Genus Angelica: A Review

et al. 2022

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Genus Angelica is one of the widely distributed and well-known genera of family Umbelliferae. It is utilized mainly by Chinese and Korean populations especially in their folk medicine. Angelica comprises a lot of medicinally important phytoconstituents such as coumarins, furanocoumarins, flavonoids, essential oils, verbascosides, polysaccharides, etc. Members of this genus play important roles, namely antioxidant, anti-inflammatory, anti-microbial, anti-diabetic, skin-whitening, cytotoxic, hepatoprotective, and many others. This review draws attention to many species of genus Angelica with much focus on A. dahurica being one of the highly medicinally used species within this genus.

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Section: Skin Whiteningmentioning

confidence: 98%

Phytochemical Constituents, Folk Medicinal Uses, and Biological Activities of Genus Angelica: A Review

et al. 2022

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“…We then investigated whether Moju has whitening activity in the B16F10 cell line. For screening of anti-melanogenic effects, B16F10 murine melanoma cells were widely used, probably because they are relatively easy to culture in vitro and share most of the melanogenic mechanisms of normal human melanocytes (Fang et al 2022). Therefore, we studied the anti-melanogenic effect of Moju and found that Moju inhibited melanin synthesis in the B16F10 cell line.…”

Section: Discussionmentioning

confidence: 99%

Anti-melanogenic effect of Moju through inhibition of tyrosinase activity

Kim

Lee

Noh

et al. 2023

Mol. Cell. Toxicol.

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Background Moju is a traditional rice beverage local to Jeonju with an alcohol content of 1–2%. Moju is made by boiling makgeolli with several kinds of medicinal herbs, such as ginger, jujube and cinnamon. The raw materials used in Moju are well known for their physiological and functional effects. Although Moju is made with functional raw materials, the operational role of Moju has not yet been reported. Objectives The aim of this study was to identify the anti-melanogenic effects of Moju in B16F10 melanoma cells and explore the potential mechanisms. Results In this study, we investigated the antioxidant activity and anti-melanogenic effect of Moju. Moju showed no toxicity to HEK293T or B16F10 cells. The antioxidant activity of Moju was confirmed by its ability to increase radical scavenging activity. Moju decreased tyrosinase activity in a concentration-dependent manner. At the cellular level, Moju reduced melanin synthesis and the expression of proteins involved in melanin synthesis at concentrations of 100, 250, and 500 μg/mL in B16F10 cells. In addition, Moju inhibited the phosphorylation of extracellular signal-regulated kinase (ERK). Conclusions These results provide evidence that Moju has antioxidant activity and anti-melanogenic effect that occur through regulation of the ERK pathway. Although further research is needed to elucidate the specific mechanism and functional components, the ability of Moju to inhibit melanin synthesis by altering tyrosinase activation suggest that it can be used as a functional whitening ingredient.

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“…Gumiganghwal-tang consists of Angelicae Dahuricae Radix (3.6 g), Osterici seu Notopterygii Radix et Rhizoma (4.5 g), Saposhnikoviae Radix (4.5 g), Cnidii Rhizoma (3.6 g), Atractylodis Rhizoma (3.6 g), Scutellariae Radix (3.6 g), Fresh Rehmanniae Radix (3.6 g), Asiasari Radix et Rhizoma (1.5 g), and Glycyrrhizae Radix et Rhizoma (1.5 g), which work synergistically to relieve cold symptoms and support immune function. Additionally, ADR is prominently used in treatments for allergic rhinitis [3], as well as in external applications for skin whitening [4] and chloasma treatment [5,6]. The pharmacological versatility of ADR is largely attributed to its rich phytochemical composition,…”

Section: Introductionmentioning

confidence: 99%

Two-Dimensional High-Performance Thin-Layer Chromatography with Bioautography for Distinguishing Angelicae Dahuricae Radix Varieties: Chemical Fingerprinting and Antioxidant Profiling

Ku,

Park,

Jang

2024

Plants

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Angelicae Dahuricae Radix (ADR) holds a prominent place in traditional medicine for its remarkable antioxidative, anti-allergic, and antiproliferative capabilities. Recognized within the Korean Pharmacopoeia (KP 12th), Angelica dahurica (Hoffm.) Benth. and Hook.f. ex Franch. and Sav. (AD) and Angelica dahurica var. formosana (H. Boissieu) Yen (ADF) serve as the botanical origins for ADR. Differentiating these two varieties is crucial for the formulation and quality control of botanical drugs, as they are categorized under the same medicinal label. This research utilized two-dimensional high-performance thin-layer chromatography (2D-HPTLC) to effectively distinguish AD from ADF. Additionally, a quantitative analysis reveals significant differences in the concentrations of key active constituents such as oxypeucedanin, imperatorin, and isoimperatorin, with AD showing higher total coumarin levels. We further enhanced our investigative depth by incorporating a DPPH bioautography, which confirmed known antioxidant coumarins and unearthed previously undetected antioxidant profiles, including byakangelicin, byakangelicol, falcarindiol in both AD and ADF, and notably, 2-linoleoyl glycerol detected only in AD as an antioxidant spot. This comprehensive approach affords a valuable tool set for botanical drug development, emphasizing the critical need for accurate source plant identification and differentiation in ensuring the efficacy and safety of herbal medicine products.

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Angelica dahurica attenuates melanogenesis in B16F0 cells by repressing Wnt/β-catenin signaling

Cited by 5 publications

References 32 publications

Phytochemical Constituents, Folk Medicinal Uses, and Biological Activities of Genus Angelica: A Review

Phytochemical Constituents, Folk Medicinal Uses, and Biological Activities of Genus Angelica: A Review

Anti-melanogenic effect of Moju through inhibition of tyrosinase activity

Two-Dimensional High-Performance Thin-Layer Chromatography with Bioautography for Distinguishing Angelicae Dahuricae Radix Varieties: Chemical Fingerprinting and Antioxidant Profiling

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