Anfärbemethoden und Dünnschicht-Chromatographie von organischen N-Nitrosoverbindungen

An Organic Chemist’s Guide to N-Nitrosamines: Their Structure, Reactivity, and Role as Contaminants

“…Particularly in the 1960s and 1970s, a number of colorimetric and fluorimetric indicators were reported for the detection of nitrosamines. 200 Similar to the more modern methods described above, these methods typically cleaved the nitrosamine N−N bond and then added an indicator which reacted with either an NO x fragment (e.g., detection of nitrite by Griess reagent) 201,202 or the secondary amine (e.g., derivatization with dansyl chloride) to give colored or fluorescent product. 203 However, these methods often had high LODs and poor selectivity, even when used as thin-layer chromatography stains rather than as stand-alone indicators.…”

“…Particularly in the 1960s and 1970s, a number of colorimetric and fluorimetric indicators were reported for the detection of nitrosamines . Similar to the more modern methods described above, these methods typically cleaved the nitrosamine N–N bond and then added an indicator which reacted with either an NO x fragment (e.g., detection of nitrite by Griess reagent) , or the secondary amine (e.g., derivatization with dansyl chloride) to give colored or fluorescent product . However, these methods often had high LODs and poor selectivity, even when used as thin-layer chromatography stains rather than as stand-alone indicators. , Other methods first reduced the nitrosamine to the corresponding hydrazine, which was then condensed with an aromatic aldehyde to give a colored or fluorescent hydrazone product. − Those methods are also incompatible with a portable in-field detection scheme, as they require harsh reagents like LAH to reduce the nitrosamine …”

N-Nitrosodimethylamine in fish meal

“…Various methods have been described for the determination of DMN in food for human and animal consumptions. These include ultraviolet (Ender et al, 1964) and infrared (Moehler and Mayrhofer, 1968), spectroscopic, polarographic (Heath and Jarvis, 1955;Walters et al, 1970), colorimetric (Eisenbrand and Preussmann, 1970), tic (Preussmann et al, 1964;Sen et a!., 1969), glc (Howard et al, 1970;, 1970 methods. Many of these methods are unsuitable for trace analysis of nitrosamines in biological mixtures mainly because of the lack of sensitivity or specificity.…”

A Critical Appraisal of the Evolution ofN-Nitrosoureas as Anticancer Drugs

“…After purification the thoroughly dried compounds should be stored in a freezer at -20 °C or lower temperature to prevent decomposition. The purity of N-nitrosoureas should be checked periodically, particularly before biological tests, by thin-layer chromatography (TLC) using either ultraviolet (UV) light or a combination of UV light and the Griess reagent 49 as convenient indicators of the nitroso moiety.…”