Anf�lligkeit verschiedener Begonien gegen�ber Fusarium foetens

Brand, Thomas; Wienberg, Jens

doi:10.1007/s10343-004-0058-3

Cited by 5 publications

(1 citation statement)

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“…Comparisons of partial DNA sequences of the EF‐1 α and β ‐tubulin gene exons and introns, the mitochondrial small subunit (mtSSU) rDNA, and Randomly Amplified Polymorphic DNA (RAPD) profiles showed that this Fusarium species is distinct in phylogeny from other Fusarium species and therefore, it was described as F. foetens (Schroers et al., 2004). This species was found for the first time in Germany in 2001 and in the USA in 2003 (Schrage, 2003; Elmer et al., 2004; Brand and Wienberg, 2005).…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Fusarium foetens in Begonia

Weerdt¹,

Zijlstra²,

Brouwershaven³

et al. 2006

Journal of Phytopathology

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Fusarium foetens is a new destructive pathogen onBegonia. This fungus is morphologically difficult to distinguish from other known Fusarium species. Moreover, the currently used assays for identification of this species require isolation and growth of the fungus which make the process time consuming. This study describes the development of a gel-based conventional polymerase chain reaction (PCR) test and a real-time PCR test for fast, practical, sensitive and specific detection of F. foetens. The gel-based PCR test presented amplifies a 275-bp fragment of the elongation factor (EF)-1a region. The real-time PCR test was based on the mitochondrial small subunit sequence of F. foetens. Both tests are suitable to detect F. foetens in cultures as well as in infected plant materials and offer potential for routine detection purposes.www.blackwell-synergy.com

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Section: Introductionmentioning

confidence: 99%