Aneuploidy patterns in drosophila melanogaster

Abstract

By using an appropriate scheme, various combinations of losses and gains of chromosomes I and II arising in oocytes of Drosophila melanogaster were obtained. The frequencies of these combinations can be arranged as special sequences or patterns which we call "aneuploidy patterns." Aneuploidy patterns were established for spontaneous, colchicine-induced, and X-ray-induced exceptions and were shown to be highly specific for the agent tested. Such patterns can be obtained very quickly, because only exceptional progeny survive. By establishing aneuploidy patterns for chemical agents one would get information of the "aneuploidizing" action of the compounds tested.

Chemically induced aneuploidy in mammalian cells: Mechanisms and biological significance in cancer

“…Our lack of understanding of possible species differences in mitosis and chromosome movement makes it important to study the effects of chemicals on aneuploidy induction in human and other mammalian cells in order to establish their risk to man. However, genetic assays for aneuploidy in mammalian cells are not well developed, and cytogenetic assays are insensitive and subject to a variety of technical problems (see below) [Galloway and Ivett, 19851. Because there have been a number of excellent reviews on aneuploidy induction in Drosophilia and fungal systems (Sankaranarayanan, 1979;Traut, 1981; Bond and Chandley, 1983;Zimmering et al, 1986; Resnick et al, 1981;Kafer et al, 1986; Griffiths et al, 19861, the present review deals primarily with chemically induced aneuploidy in mammalian cells. We will discuss the biological significance of aneuploidy, including the cellular and molecular effects of numerical chromosome changes, and the biological consequences of these changes.…”

Aneuploidy induced by bleomycin in oocytes of Drosophila melanogaster: Studies with the aneuploidy pattern method

“…The old control pattern consists of 617 aneuploid exceptions (classes I to VI), the new pattern of 596, and the combined pattern, therefore, of 617 + 596 = 1,213 aneuploid exceptions. It can be estimated (see Traut [1981] for details) that the combined control patlern is based on about 6.3 x lo6 individuals. The combination of the old and the new control pattern is legitimate, because both patterns agree well with each other (P = 0.45).…”

“…The statistical test applied throughout this communication is based on the calculation of x2 with the aid of an r X c contingency table (see Traut [1981] for details). All experiments were performed at 25°C.…”

The solvent dimethylsulfoxide (DMSO) does not induce aneuploidy in oocytes of drosophila melanogaster

“…Also the difference between the DMSO and the control pattern is not significant (P = .a), ie, there is no evidence for the induction of aneuploidy by DMSO. The statistical test applied is based on the calculation of x2 with the aid of an r x c contingency table [see 10 for details]. The DMSO pattern and the control pattern presented graphically in Figure 1 are very similar.…”