Anesthetic preconditioning increases sirtuin 2 gene expression in a renal ischemia reperfusion injury model

Echavarría, Raquel; García, David Suárez; Figueroa, Francisco; Franco-Acevedo, Adriana; Palomino, J.C.; Portilla-De-Buen, Eliseo; Goldaraz-Monraz, María de la Paz; Moreno-Carranza, Bibiana; Melo, Zesergio

doi:10.23736/s0393-2249.19.03361-7

Cited by 13 publications

(11 citation statements)

References 29 publications

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“…This molecule has been proposed as a sensitive biomarker of acute renal tubular injury [ 28 ], and its mRNA expression associates with interstitial fibrosis and tubular atrophy in kidney transplant recipients [ 29 ]. As expected from our previous studies [ 30 ], IRI-dependent induction of KIM-1 mRNA expression was increased after IRI in the ischemia-reperfusion treated kidneys. This increase was prevented by morphine and fentanyl preconditioning ( Figure 2 B).…”

Section: Resultssupporting

confidence: 90%

Opioid Preconditioning Modulates Repair Responses to Prevent Renal Ischemia-Reperfusion Injury

et al. 2020

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Progression to renal damage by ischemia-reperfusion injury (IRI) is the result of the dysregulation of various tissue damage repair mechanisms. Anesthetic preconditioning with opioids has been shown to be beneficial in myocardial IRI models. Our main objective was to analyze the influence of pharmacological preconditioning with opioids in renal function and expression of molecules involved in tissue repair and angiogenesis. Experimental protocol includes male rats with 45 min ischemia occluding the left renal hilum followed by 24 h of reperfusion with or without 60 min preconditioning with morphine/fentanyl. We analyzed serum creatinine and renal KIM-1 expression. We measured circulating and intrarenal VEGF. Immunohistochemistry for HIF-1 and Cathepsin D (CTD) and real-time PCR for angiogenic genes HIF-1α, VEGF, VEGF Receptor 2 (VEGF-R2), CTD, CD31 and IL-6 were performed. These molecules are considered important effectors of tissue repair responses mediated by the development of new blood vessels. We observed a decrease in acute renal injury mediated by pharmacological preconditioning with opioids. Renal function in opioid preconditioning groups was like in the sham control group. Both anesthetics modulated the expression of HIF-1, VEGF, VEGF-R2 and CD31. Preconditioning negatively regulated CTD. Opioid preconditioning decreased injury through modulation of angiogenic molecule expression. These are factors to consider when establishing strategies in pathophysiological and surgical processes.

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Section: Resultssupporting

confidence: 90%

Opioid Preconditioning Modulates Repair Responses to Prevent Renal Ischemia-Reperfusion Injury

et al. 2020

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“…Interestingly, upregulation of sirtuins (SIRT1/SIRT2) in the nucleus accumbens-the subcortical region of brain most implicated in drug addictionhas been observed in both morphine-and cocaine-administered mice [26]. Additionally, treatment with the opioids morphine and fentanyl has been shown to increase SIRT2 expression [27]. Ephrin receptor signaling, which has been implicated in the regulation of embryonic development, axon guidance [28], and cellular processes such as long-term potentiation in adulthood [29] was upregulated in the neuroglial cultures treated with oxy.…”

Section: Discussionmentioning

confidence: 99%

Integrated Systems Analysis of Mixed Neuroglial Cultures Proteome Post Oxycodone Exposure

Guda

Odegaard

Tan

et al. 2021

IJMS

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Opioid abuse has become a major public health crisis that affects millions of individuals across the globe. This widespread abuse of prescription opioids and dramatic increase in the availability of illicit opioids have created what is known as the opioid epidemic. Pregnant women are a particularly vulnerable group since they are prescribed for opioids such as morphine, buprenorphine, and methadone, all of which have been shown to cross the placenta and potentially impact the developing fetus. Limited information exists regarding the effect of oxycodone (oxy) on synaptic alterations. To fill this knowledge gap, we employed an integrated system approach to identify proteomic signatures and pathways impacted on mixed neuroglial cultures treated with oxy for 24 h. Differentially expressed proteins were mapped onto global canonical pathways using ingenuity pathway analysis (IPA), identifying enriched pathways associated with ephrin signaling, semaphorin signaling, synaptic long-term depression, endocannabinoid signaling, and opioid signaling. Further analysis by ClueGO identified that the dominant category of differentially expressed protein functions was associated with GDP binding. Since opioid receptors are G-protein coupled receptors (GPCRs), these data indicate that oxy exposure perturbs key pathways associated with synaptic function.

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“…Opioids were reported of having gastric mucosal in acute stress by several pieces of research [9,[11][12][13]. The drugs including morphine, fentanyl and sufentanil.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that the prophylactic use of opioids can reduce the stress state of patients in surgery, attenuating ischemiareperfusion in heart [9,10], kidney [11][12][13], liver [14] and gastric mucous membrane, leading to a reduction in stress-related injuries, including gastric mucosal damage. Studies have reported that pretreatment with fentanyl or sufentanil before stress attenuates ischemia-reperfusion damage [9,10,13,14].…”

Section: Introductionmentioning

confidence: 99%

“…A series of studies have shown that the ischemic preconditioning of the distal limbs has a certain protective effect on the distal organs, such as heart [18], lung, liver, kidney [19], and brain [18,20,21]. The main mechanism of the protective effect of Limb ischemic preconditioning (LIPc) may include improving liver energy metabolism and microcirculation, reducing free radical production, inhibiting the activation of inflammatory cells and apoptosis, and inducing the release of endogenous protective substances [8,12,14,22,23]. We speculate that LIPc can also have some protective effects on acute gastric mucosal lesions.…”

Section: Introductionmentioning

confidence: 99%

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Limb Ischemic Preconditioning or Dezocine Pre-Treatment will Reduce Acute Gastric Mucosal Injury in Rats

Dou¹

2021

BJSTR

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Objective: The proposal of the studying was to investigate the protective effect of Limb ischemic precondition or Dezocine pre-treatment in acute stress-related gastric mucosal injury.Methods: Forty-five Sprague Dawley (SD) male rats at 2-3 month were randomly divided into three groups: controlled group (Group C, n=15), Ischemic precondition group (Group T, n=15): rats in this group received 5 cycles of ischemic precondition of 5-5 min in their left legs; Dezocine pre-treatment group (Group D, n=15): Rats in this group received Dezocine subcutaneous injection at a dosage of 10µg/g. Rats were beheaded after 6h of cold water immerse, and stomach was taken out for Gastric injury index (GI), HE stains, and measurement of MDA, SOD, MPA. Result:(1) There were significant lower GI scores and less Inflammatory response in gastric (fewer Neutrophils) in Group T and Group D when compared to Group C, (p<0.05, CI=95%);(2) There levers of MDA and MPA were significantly lower in Group T and Group D when compared to That of Group C,(p<0.05, CI=95%) and levers of SOD in Group T and Group D were significantly higher than that of Group C, (p<0.05, CI=95%);(3) Lever of SOD in Group T was higher than that in Group D,(P=0.048, CI=95%). Conclusion:Both Limb ischemic preconditioning and Dezocine pre-treatment will reduce acute gastric mucosal injury by inhibiting the inflammatory response and cleaning oxygen radical.

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Anesthetic preconditioning increases sirtuin 2 gene expression in a renal ischemia reperfusion injury model

Cited by 13 publications

References 29 publications

Opioid Preconditioning Modulates Repair Responses to Prevent Renal Ischemia-Reperfusion Injury

Opioid Preconditioning Modulates Repair Responses to Prevent Renal Ischemia-Reperfusion Injury

Integrated Systems Analysis of Mixed Neuroglial Cultures Proteome Post Oxycodone Exposure

Limb Ischemic Preconditioning or Dezocine Pre-Treatment will Reduce Acute Gastric Mucosal Injury in Rats

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