Anesthetic experimental device for small animal

Abstract

Purpose:The difficulty to anesthetize small laboratory animals with vaporizer prompted us to go in search of new materials, and create new techniques. The improved equipment of anesthesia we looked for should be low cost, practical, versatile, and its management serve ethical, teaching, and research purposes. Methods: The new components of the equipment were: the vaporizer, the unidirectional valve, the glass cylinder filled with water, the flow guidance y-shape tube, the flow regulators, the mask modifications, and another free airway for emergency occurrence. A test was done with 30 Wistar rats, Rattus norvegicus albinus, divided into three groups with 10 rats for each one. Groups 1, 2 and 3 were anesthetized with Ether, Halothane and Sevoflurane respectively, using the new gadget. The anesthetic induction time, the breathing rhythm alteration during an anesthesia pre-established time (10 minutes), and the recovery time were observed. Results: The equipment enabled an easy handling, and fulfilled a larger safeness and stability during the induction and anesthetic management. The test showed it was possible to make use of several anesthetic agents. Conclusion:The device is effective, and turns the anesthesia procedure into a very easy practice with low-cost. It should be recommended for experimental surgery, teaching and research. Key words: Anesthesia, Inhalation. Equipment Design. Experimental Development. Rats. RESUMO Objetivos:As dificuldades evidenciadas no uso de vaporizadores para anestesia em animais de pequeno porte motivaram o aperfeiçoamento e a criação de novos componentes técnicos visando a construção de um aparelho de anestesia de baixo custo, prático e versátil no manuseio e que atenda aos preceitos éticos do ensino e da pesquisa. Métodos: Utilizaram-se 30 ratos da linhagem Wistar, Rattus norvegicus albinus, distribuídos em três grupos. Grupo 1,2 e 3 (n=10, cada), compreenderam o uso do novo aparelho aduzido, respectivamente, do Éter; Halotano e Sevoflurano. Foram verificados o tempo de indução anestésica, as alterações do ritmo ventilatório durante o tempo de manutenção anestésica (pré-estabelecido em 10 min) e o tempo de recuperação anestésica. O aparelho foi construído com dispositivos técnicos novos sobressaindo: o vaporizador, a válvula unidirecional na campânula, o cilindro de vidro com água, o tubo direcionador de fluxo em forma de Y, os reguladores de fluxo, as modificações na máscara e a entrada de ar, independente, para situações emergenciais. Resultados: O aparelho permitiu um fácil manuseio e propiciou uma maior segurança e estabilidade na indução e manutenção anestésicas possibilitando, inclusive, a utilização de vários agentes anestésicos. Conclusão: O aparelho demonstrou ser útil para anestesia em cirurgia experimental, facilitando a realização dos procedimentos de pesquisa e de ensino de forma mais prática, eficiente, segura, e com baixo custo. Descritores: Anestesia por Inalação. Desenho de Equipamento. Desenvolvimento Experimental. Ratos.

Different inoculum loads of Toxoplasma gondii induce reduction of myenteric neurons of the rat colon

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Oral dependent-dose toxoplasmic infection model induced by oocysts in rats: Myenteric plexus and jejunal wall changes

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Inhalation anesthesia equipment for rats with provision of simultaneous anesthetic and oxygen

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