Anesthetic activity of plant essential oils on &lt;i&gt;Cyprinus carpio&lt;/i&gt; (koi carp)

Khumpirapang, Nattakanwadee; Pikulkaew, Surachai; Anuchapreeda, Songyot; Okonogi, Siriporn

doi:10.5582/ddt.2017.01068

Cited by 10 publications

(5 citation statements)

References 51 publications

(45 reference statements)

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“…Essential oils from certain plants possess several important biological activities [12][13][14]. Among those activities, the antimicrobial activity of plant essential oils is one of the most interesting issues to be focused on because they might be useful as the natural antimicrobial alternatives to be used instead of the harmful chemical antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

et al. 2021

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The aim of the present study was to develop a microemulsion (ME) containing Alpinia galanga oil (AGO), 1,8-cineole (C), or methyl eugenol (M) as an active pharmaceutical ingredient (API) for enhancing their antimicrobial activities. Agar diffusion, broth microdilution, and killing kinetics were used for antimicrobial evaluations. The ME composed of 30% API, 33.4% Tween 80, 16.6% ethanol, and 20% water appeared as translucent systems with droplet size and polydispersity index of 101.1 ± 1.3 nm and 0.3 ± 0.1, 80.9 ± 1.1 nm and 0.4 ± 0.1, and 96.6 ± 2.0 nm and 0.2 ± 0.1 for ME-AGO, ME-C, and ME-M, respectively. These ME formulations showed minimum bacterial concentrations of 3.91–31.25 µg/mL and 50% fungal inhibition concentrations of 1.83 ± 0.27–0.46 ± 0.13 µg/mL, 2–4 times stronger, and faster kinetic killing rate than their respective API alone. Keeping the ME formulations at 4 C, 25 C, and 40 C for 12 weeks did not affect their activities against fungi and Gram-negative bacteria, but the high temperature of 40 C decreased their activities against Gram-positive bacteria. It is concluded that ME is a promising delivery system for AGO and its major compounds to enhance their water miscibility and antimicrobial activities.

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Section: Introductionmentioning

confidence: 99%

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

et al. 2021

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“…These experiments revealed that koi carp can reach stage 3 anesthesia upon exposure to essential oils from Ocimum sp. at a concentration of 100 mg/L and Alpinia galanga at a concentration of 200 mg/L, which contain eugenol, 1,8-cineole, and various other compounds [5,7]. Research on plant extracts holds the potential to advance the development of effective anesthetic formulations for aquatic animals.…”

Section: Discussionmentioning

confidence: 99%

“…Eugenol, an aromatic oil extracted from clove (Syzygium aromaticum), exhibits potential applications in aquatic animals and has been extensively used in many research projects [3,4]. Several plants, including Ocimum basilicum, O. canum, and O. sanctum, have been extracted for their essential oils to study their anesthetic properties in fish [5]. Recently, 1,8-cineole (eucalyptol), a monoterpene cyclic ether abundantly found in nature, has been reported to have anesthetic effects in many fish species, such as common carp [6], koi carp [7], and Nile tilapia [8].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect between Eugenol and 1,8-Cineole on Anesthesia in Guppy Fish (Poecilia reticulata)

Nuanmanee,

Sriwanayos,

Boonyo

et al. 2024

Veterinary Sciences

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This study aimed to evaluate the synergistic effect between eugenol and 1,8-cineole on anesthesia in female guppy fish (Poecilia reticulata). Experiment I evaluated the concentrations of 0, 12.5, 25, 50, and 75 mg/L of eugenol and 0, 100, 200, 300, and 400 mg/L of 1,8-cineole for times of induction and recovery from anesthesia. Experiment II divided fish into 16 study groups, combining eugenol and 1,8-cineole in pairs at varying concentrations, based on the dosage of the chemicals in experiment I. The results of the anesthesia showed that eugenol induced fish anesthesia at concentrations of 50 and 70 mg/L, with durations of 256.5 and 171.5 s, respectively. In contrast, 1,8-cineole did not induce fish anesthesia. In combination, using eugenol at 12.5 mg/L along with 1,8-cineole at 400 mg/L resulted in fish anesthesia at a time of 224.5 s. Increasing the eugenol concentration to 25 mg/L, combined with 1,8-cineole at 300 and 400 mg/L, induced fish anesthesia at times of 259.0 and 230.5 s, respectively. For treatments with eugenol at 50 mg/L combined with 1,8-cineole at 100 to 400 mg/L, fish exhibited anesthesia at times of 189.5, 181.5, 166.0, and 157.5 s. In the case of eugenol at 75 mg/L, fish showed anesthesia at times of 175.5, 156.5, 140.5, and 121.5 s, respectively. The testing results revealed that 1,8-cineole as a single treatment could not induce fish anesthesia. However, when supplementing 1,8-cineole in formulations containing eugenol, fish exhibited a significantly faster induction of anesthesia (p < 0.05). Furthermore, all fish that underwent anesthesia were able to fully recover without any mortality. However, the shorter anesthesia duration resulted in a significantly prolonged recovery time. In conclusion, eugenol and 1,8-cineole work better together as anesthetics than when used separately, and demonstrated the safety of using these anesthetic agents on guppy fish.

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“…The anesthetic effectiveness of other Piper plants have been demonstrated in some fishes, as the oil of Alpinia galanga L. (Willd) for Cyprinus carpio (Linnaeus, 1758) (Khumpirapang et al, 2018) and the essential oil of P. divaricatum (Mey) in C. macropomum juveniles (Vilhena et al, 2019), but they were mainly showed for mammals as rats (Sell and Carlini, 1976), rabbits (Barbosa, 1988;Carlini et al, 1981) and mice (Yano et al, 2006). Methyleugenol a substance that is present in the essential oils of different Piper plants is also an agonist of GABA A receptors (Ding et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Anesthesia or seizure-like behavior? The effects of two Amazonian plants, Acmella oleracea and Piper alatabaccum in zebrafish (Danio rerio)

et al. 2022

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Currently, available fish anesthetics can produce important side effects, including respiratory arrest and distress. Easy-to-implement alternatives with low toxicity are needed to ensure fish health as well as to help artisanal fisheries and fish sellers in handling and transporting fishes, and native plants seems to be the best alternative. We aimed to implement an anesthetic protocol using crude ethanolic extracts from flowers and leaves of two Amazonian plants, the Acmella oleracea and Piper alatabaccum. We first tested the extracts for anesthesia, using the zebrafish as model. Even though in some treatments the animals apparently entered deep anesthesia, many of them presented aberrant behaviors and even died. Thus, we performed new experiments testing the extracts effects on seizure-like behaviors of the fish. Only the leaf extract of A. oleracea has potential effects for fish anesthesia. Both the flower extract from this plant and the leaf extract from P. alatabaccum induced seizure-like behavior in the animals. In conclusion, besides bringing a possible new anesthetic protocol for fish, our work draws attention for the neurotoxic effects the anesthetic solutions may cause, since several studies defend other Piper species as anesthetic for fish and A. oleracea flowers’ extract was already pointed as fish anesthetic.

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Anesthetic activity of plant essential oils on <i>Cyprinus carpio</i> (koi carp)

Cited by 10 publications

References 51 publications

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

Development of Microemulsion Containing Alpinia galanga Oil and Its Major Compounds: Enhancement of Antimicrobial Activities

Synergistic Effect between Eugenol and 1,8-Cineole on Anesthesia in Guppy Fish (Poecilia reticulata)

Anesthesia or seizure-like behavior? The effects of two Amazonian plants, Acmella oleracea and Piper alatabaccum in zebrafish (Danio rerio)

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