Anemia in prospective blood donors deferred by the copper sulphate technique of hemoglobin estimation

Antwi‐Baffour, Samuel; Annor, David Kwasie; Adjei, Jonathan Kofi; Kyeremeh, Ransford; Kpentey, George; Kyei, Foster

doi:10.1186/s12878-015-0035-3

Cited by 11 publications

(17 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, the most common anemias in Libreville blood donors were normocytic normochromic (39.4%) and normocytic hypochromic (23.6%). The highest prevalence of normocytic normochromic anemia observed in Libreville blood donors is consistent with the prevalence of 46.74% reported previously in Ghana [30]. The distribution of these two types of anemia was not identical in both sexes.…”

Section: Discussionsupporting

confidence: 89%

Hemogram abnormalities in apparently healthy first-time blood donors in Libreville, Gabon.

BISSEYE

Ndong

Mombo

et al. 2019

SJMS

View full text Add to dashboard Cite

Background: The objective of this study was to determine complete blood count (CBC) abnormalities in Libreville blood donors to advocate for hemoglobin pre-donation implementation and to take into account CBC results in blood donation qualification. Methods: This retrospective study was conducted with 4573 blood donors in March 2016 and from January to April 2017. CBC was performed using SysmexXP-300TM hematology analyzer (SYSMEX Corporation, Kobe, Japan). Results: Blood donors were predominantly males (83.7%) with an age ranging from 18 to 59 years. The abnormalities of leukocyte, platelet and erythrocyte counts were determined in blood donors. Leukopenia and thrombocytopenia were significantly more common in men than women (29.02% vs 24.4%, p = 0.011 and 16.2% vs 7.5%, p <0.001). Only 1.0% of women and 0.84% of men have leukocytosis, and 0.7% of women and 0.2% of men have thrombocytosis. Anemia was significantly more common in women compared to men (69.4% vs. 45.0%, P <0.001). Normocytic normochromic and normocytic hypochromic anemia were most common among Libreville blood donors with 39.4% and 23.6%; followed by microcytic normochromic (18.7%) and microcytic hypochromic (13.2%) anemia. Normocytic normochromic and normocytic hypochromic anemia were significantly more common in men than in women, whereas microcytic normochromic anemia was more prevalent among women compared to men (34.6% vs. 13.9%, p <0.001). Conclusions: The results of this study clearly show the need to perform a pre-donation hemoglobin test in blood donors and to take into account their hemogram in the blood donation selection process at the Libreville National Blood Transfusion Center. Keywords: Hemogram, Anemia, Blood Donors, Libreville, Gabon.

show abstract

Section: Discussionsupporting

confidence: 89%

Hemogram abnormalities in apparently healthy first-time blood donors in Libreville, Gabon.

BISSEYE

Ndong

Mombo

et al. 2019

SJMS

View full text Add to dashboard Cite

show abstract

“…77–80 All studies reported lower mean Hb concentrations (2.0–16.0 g/L) in venous blood analyzed by the CST compared with the reference (Fig. 2A).…”

Section: Resultsmentioning

confidence: 93%

“…2A). When applying the ±7% threshold for mean difference for the studies comparing the CST with the reference, two studies were within the threshold range 78,79 and two studies exceeded the ±7% bias 77,80 (Fig. 2A).…”

Section: Resultsmentioning

confidence: 99%

“…Figure 2A shows four studies where venous blood analyzed by the reference was compared with the CST among infants and children aged 6 months to 6 years, adult males, females, and PW aged 18 years and older. [77][78][79][80] All studies reported lower mean Hb concentrations (2.0−16.0 g/L) in venous blood analyzed by the CST compared with the reference ( Fig. 2A).…”

Section: Comparability Of the Automated Hematology Analyzer (Referencmentioning

confidence: 99%

See 1 more Smart Citation

Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings

Whitehead

Mei

Mapango

et al. 2019

Annals of the New York Academy of Sciences

118

100

View full text Add to dashboard Cite

This paper describes and compares methods and analyzers used to measure hemoglobin (Hb) in clinical laboratories and field settings. We conducted a literature review for methods used to measure Hb in clinical laboratories and field settings. We described methods to measure Hb and factors influencing results. Automated hematology analyzer (AHA) was reference for all Hb comparisons using evaluation criteria of ±7% set by College of American Pathologists (CAP) and Clinical Laboratory Improvement Amendments (CLIA). Capillary fingerprick blood usually produces higher Hb concentrations compared with venous blood. Individual drops produced lower concentrations than pooled capillary blood. Compared with the AHA: (1) overall cyanmethemoglobin (1.0–8.0 g/L), WHO Colour Scale (0.5–10.0 g/L), paper-based devices (5.0–7.0 g/L), HemoCue® Hb-201 (1.0–16.0 g/L) and Hb-301 (0.5–6.0 g/L), and Masimo Pronto® (0.3–14.0 g/L) overestimated concentrations; (2) Masimo Radical® −7 both under- and overestimated concentrations (0.3–104.0 g/L); and (3) other methods underestimated concentrations (2.0–16.0 g/L). Most mean concentration comparisons varied less than ±7% of the reference. Hb measurements are influenced by several analytical factors. With few exceptions, mean concentration bias was within ±7%, suggesting acceptable performance. Appropriate, high-quality methods in all settings are necessary to ensure the accuracy of Hb measurements.This paper describes and compares methods and analyzers used to measure hemoglobin (Hb) in clinical laboratories and field settings. With few exceptions, mean concentration bias was within ±7%, suggesting acceptable performance. Appropriate, high-quality methods in all settings are necessary to ensure the accuracy of Hb measurements.

show abstract

“…This was highlighted in this study in which the study site that employed the copper sulfate hemoglobin screening protocol had significantly reduced serum ferritin levels compared to the other regions where automated hemoglobin estimations were employed. As the copper sulfate density-based hemoglobin screening procedure has been demonstrated to be fraught with low sensitivity in detecting negative iron balance,[ 19 20 ] blood donor recruitment centers should be encouraged to adopt hemoglobin meters for hemoglobin screening during mobile sessions to ensure adequate protection of prospective donors. [ 21 ]…”

Section: Discussionmentioning

confidence: 99%

Depleted iron stores in voluntary blood donors: A three-center cross-sectional study in Ghana

Adu

Bennin

Edzie

et al. 2020

Asian J Transfus Sci

View full text Add to dashboard Cite

BACKGROUND: Blood donation is frequently associated with iron deficiency. Although iron deficiency is endemic in Ghana, there is a scarcity of data on iron stores in blood donors to inform donor recruitment policy. This study determined the prevalence and factors predictive of depleted iron stores in blood donors. MATERIALS AND METHODS: This cross-sectional study recruited 287 blood donors from three regions in Ghana. Venous blood samples were collected for estimation of C-reactive protein, full blood count, and serum ferritin. Questionnaires were used to capture sociodemographic data. Data were analyzed using SPSS or GraphPad Prism. Multivariate logistic regression and receiver operator characteristics (ROC) analyses were, respectively, used to determine the factors associated with depleted iron stores or sensitivities of calculated red cell indices in predicting depleted iron stores in the participants. RESULTS: Whereas 27.4% of the blood donors had depleted iron stores (ferritin <15 ng/dL), only 11% took iron supplementation. While ferritin levels significantly increased with age, 49.5% of the blood donors were aged 20–29 years. Whereas 39.5% of participants had never donated blood, 24.9% had donated ≥3 units of whole blood in the past 2 years. Female (adjusted odds ratio [aOR]: 7.407, P = 0.005), multiple previous donations (1–2 [aOR: 1.846, P = 0.431]; ≥3 [aOR: 6.297, P = 0.016]), no iron supplementation (aOR: 17.553, P = 0.078), or platelet count ≥150 × 10 9 /L (aOR: 2.689, P = 0.354) significantly associated with iron depletion. ROC analyses showed that whereas mean cell hemoglobin (MCH) density (area under the curve [AUC]: 0.735, P < 0.01), MCH (AUC: 0.772, P < 0.01) or Shine and Lal (AUC: 0.736, P < 0.01) fairly predicted iron depletion, combined cell index (AUC: 0.660, P < 0.01) or Green and King (AUC: 0.603, P < 0.01) indices poorly predicted iron depletion. CONCLUSIONS: More than quarter of voluntary blood donors suffers postdonation sideropenia. Calculated red cell indices should be investigated in different settings to validate usefulness in detecting iron depletion.

show abstract

Anemia in prospective blood donors deferred by the copper sulphate technique of hemoglobin estimation

Cited by 11 publications

References 18 publications

Hemogram abnormalities in apparently healthy first-time blood donors in Libreville, Gabon.

Hemogram abnormalities in apparently healthy first-time blood donors in Libreville, Gabon.

Methods and analyzers for hemoglobin measurement in clinical laboratories and field settings

Depleted iron stores in voluntary blood donors: A three-center cross-sectional study in Ghana

Contact Info

Product

Resources

About