Preterm labor is one of the major concerned due to high death rate among children less than 5 years of age. According to a survey report from UNICEF in 2016, Pakistan stands on second number amongst the top ten countries where the death rate due to complications in premature birth is two-third. Preterm labor has been linked with various morbidities among which one is anemia. Premature infants suffer from anemia due to increased iron demand, loss of blood because of phlebotomy and physiological drop-off in the level of hemoglobin Assessment of various blood profile and their role in the development of anemia in preterm infants.Hundred premature infants and hundred normal infants were taken as healthy control. For the assessment 5ml blood was taken and serum was separated and stored at -75 degrees for the biochemical analysis. Subjects were determined for the number of biochemical and physiological parameters such as Complete Blood Count (CBC) with the help of automated Coulter by Beckman Coulter, Inc.Mean body weight (Kg) and age (YRS) of preterm anemic infants was 1.36±0.645 and 27.56±4.26 in comparison with full-term infants 2.87±0.913 and 37.59±5.26 respectively. The