Andrology: Total antioxidant capacity of human seminal plasma

Smith, Rosita; Vantman, David; Ponce, Jose; Escobar, Jorge; Lissi, Eduardo

doi:10.1093/oxfordjournals.humrep.a019465

Cited by 168 publications

(92 citation statements)

References 8 publications

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“…Evidence has suggested that a low seminal Total Antioxidant Capacity (TAC) was related to the male infertility [14,[23][24][25]. The values for the TAC were significantly reduced in the subjects with impaired seminograms.…”

Section: The Routine Semen Analysismentioning

confidence: 99%

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

Pahune

Choudhari²,

Muley³

2013

JCDR

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Background: There are growing evidences that the damage which is caused to the spermatozoa by the Reactive Oxygen Species (ROS) plays a key role in the male infertility. The seminal plasma is endowed with many enzymatic and nonenzymatic antioxidants which protect the spermatozoa against oxidative stress.The present study was undertaken by using a simple, colourimetric, ferric reducing, antioxidant power for assessing the total antioxidant power rather than the individual antioxidants. The measurement of the individual antioxidants in the seminal plasma, such as Superoxide Dismutase, Vitamin E, etc. is time consuming, which often requires sophisticated and expensive techniques and these measurements may not correlate with the quality of semen. Aim:To evaluate the total antioxidant capacity of seminal plasma by estimating the Ferric Reducing Antioxidant Power (FRAP) of semen in different groups of subjects and to correlate it with the different seminogram parameters. Material and Methods:The semen samples were obtained from 150 male partners of infertile couples who attended the Reproductive Biology Unit (Infertility Clinic) of the Department of Physiology, MGIMS, Sevagram, who were aged 20-58 years and they were analyzed for the routine seminogram parameters. All the subjects were categorized into two main groups, A. The subjects with abnormal ejaculates, who were further sub classified into the following groups i) Asthenoteratozoospermics (n=25) ii) Oligoasthenoteratozoospermics (n=26) and iii) Azoospermics (n=19) and B. The subjects with normal ejacu lates (n=80). The total antioxidant power was measured spectro photometrically by using the Ferric Reducing Antioxidant Power (FRAP) assay. Results:The Total Antioxidant Capacity (TAC) was found to be significantly lower in the abnormal ejaculates than in the normal ejaculates. A statistically significant positive correlation was observed between the TAC and all the seminogram parameters such as the sperm concentration, sperm motility and sperm morphology (p<0.05). Conclusion:A decreased seminal plasma antioxidant capacity (TAC) could have significant role in the aetiology of impaired sperm functions. So, the TAC may be used as specific biomarker for assessing the oxidative stress in sperms.

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Section: The Routine Semen Analysismentioning

confidence: 99%

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

Pahune

Choudhari²,

Muley³

2013

JCDR

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“…20,27,28 However, it is unclear whether reduced semen antioxidant capacity necessarily causes sperm dysfunction (including sperm DNA damage). 1,3,29,30 Indeed, there is some controversy as to whether the high ROS levels detected in the semen of infertile men are due to increased ROS production, decreased ROS scavenging capacity or both.…”

Section: 14mentioning

confidence: 99%

“…6,15,16 Seminal plasma contains a number of high-molecular weight enzymatic antioxidants (superoxide dismutase, catalase and glutathione peroxidase) and a deficiency in these enzymes has been reported to cause sperm DNA damage and male infertility. 1,7,10, [17][18][19] Seminal fluid also contains non-enzymatic antioxidants (ascorbic acid, a-tocopherol, pyruvate, glutathione, Lcarnitine, taurine and hypotaurine) [20][21][22][23] which constitute the bulk of seminal antioxidant capacity. In addition, urate, 24 pyruvate, 11,25 albumin, beta carotenes and ubiquinol 26 have been detected in seminal plasma.…”

Section: Relationship Between Oxidative Stress and Sperm Dysfunctionmentioning

confidence: 99%

Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

110

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Infertile men have higher levels of semen reactive oxygen species (ROS) than do fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This observation has led clinicians to treat infertile men with antioxidant supplements. The purpose of this article is to discuss the rationale for antioxidant therapy in infertile men and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm function and DNA damage. To date, most clinical studies suggest that dietary antioxidant supplements are beneficial in terms of improving sperm function and DNA integrity. However, the exact mechanism of action of dietary antioxidants and the optimal dietary supplement have not been established. Moreover, most of the clinical studies are small and few have evaluated pregnancy rates. A beneficial effect of in vitro antioxidant supplements in protecting spermatozoa from exogenous oxidants has been demonstrated in most studies; however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established conclusively. Keywords: antioxidant; male infertility; oxidative stress; sperm DNA fragmentation; vitamins RELATIONSHIP BETWEEN OXIDATIVE STRESS AND SPERM DYSFUNCTION Seminal oxidative stress (OS) results from an imbalance between reactive oxygen species (ROS) production and ROS scavenging by seminal antioxidants. Seminal OS is believed to be one of the main factors in the pathogenesis of sperm dysfunction and sperm DNA damage in male infertility.1-4 Indeed, it is estimated that 25% of infertile men possess high levels of semen ROS, whereas fertile men do not have high levels of semen ROS.1,4-6 Although a controlled production of these ROS is required for sperm physiology (sperm hyperactivation, capacitation and acrosome reaction) and for natural fertilization, [7][8][9] the excessive production of ROS by immature germ cells and leukocytes causes sperm dysfunction (lipid peroxidation, loss of motility and sperm DNA damage). 9,10 Spermatozoa are particularly susceptible to oxidative injury due to the abundance of plasma membrane polyunsaturated fatty acids. [10][11][12] These unsaturated fatty acids provide fluidity that is necessary for membrane fusion events (e.g., the acrosome reaction and sperm-egg interaction) and for sperm motility. However, the unsaturated nature of these molecules predisposes them to free radical attack and ongoing lipid peroxidation throughout the sperm plasma membrane. Once this process has been initiated, accumulation of lipid peroxides occurs on the sperm surface (this results in loss of sperm motility) and oxidative damage to DNA can ensue. 13,14SEMINAL ANTIOXIDANT CAPACITY AND SPERM DYSFUNCTION Seminal plasma and spermatozoa themselves are well endowed with an array of protective antioxidants to protect spermatozoa from OS, especially, at the post-testicular level. 6,15,16 Seminal plasma contains a number of high-molecular weigh...

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“…Some studies have shown that a deficiency in semen antioxidants is associated with sperm DNA damage, whereas, other studies have not observed such a relationship [6,[36][37][38][39]. Similarly, some studies have found that seminal antioxidant activity is reduced in infertile men with high levels of seminal ROS (relative to those with normal levels of ROS) whereas others have not shown this [14,[40][41][42]. To date, there are no studies to indicate a relationship between systemic antioxidant or vitamin deficiency and male infertility.…”

Section: Semen Antioxidants and Sperm Dna Damagementioning

confidence: 99%

Antioxidants and sperm DNA damage: a clinical perspective

Zini

Gabriel

Baazeem

2009

J Assist Reprod Genet

175

110

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Purpose Infertile men possess substantially more sperm DNA damage than do fertile men, damage that may impact negatively on reproductive outcomes. In this era of assisted reproductive technologies there is mounting concern regarding the safety of utilizing DNA-damaged spermatozoa in this setting. Therefore, it is important to identify strategies that may reduce sperm DNA damage. The purpose of this review is to discuss the rationale for antioxidant therapy in men with sperm DNA damage and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm DNA damage. Methods We reviewed the literature on antioxidants and sperm DNA damage. Results To date, the data suggest that dietary antioxidants may be beneficial in reducing sperm DNA damage, particularly, in men with high levels of DNA fragmentation. However, the mechanism of action of dietary antioxidants has not been established and most of the clinical studies are small. A beneficial effect of in vitro antioxidant supplements in protecting sperm DNA from exogenous oxidants has been demonstrated, however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established.

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Andrology: Total antioxidant capacity of human seminal plasma

Cited by 168 publications

References 8 publications

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

The Total Antioxidant Power of Semen and Its Correlation with the Fertility Potential of Human Male Subjects

Antioxidant therapy in male infertility: fact or fiction?

Antioxidants and sperm DNA damage: a clinical perspective

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