Andrology: Effects of nitric oxide on human spermatozoa: evidence that nitric oxide decreases sperm motility and induces sperm toxicity

Rosselli, Marinella; Dubey, Raghvendra K.; Imthurn, Bruno; Macas, Ervin; Keller, Paul J.

doi:10.1093/oxfordjournals.humrep.a136174

Cited by 199 publications

(147 citation statements)

References 19 publications

(40 reference statements)

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“…Accordingly, human sperm motility is inhibited by the NOS inhibitor N G -nitro-L-arginine methyl ester and by the NO scavenger methylene blue (Lewis et al 1996, Donnelly et al 1997. On the other hand, high NO concentrations (25-125 mM pure NO, 0.25-2.5 mM sodium nitroprusside, 12-600 mM S-nitroso-N-acetylpenicillamine, and 100-125 mM 3-morpholinosydnonimine) seem to exert opposite effects on the motility of human spermatozoa in vitro (Rosselli et al 1995, Weinberg et al 1995, Nobunaga et al 1996. Studies on sperm capacitation showed that NO (1-100 mM spermine NONOate (SPNO) or diethylamine-NONOate) increases cAMP levels, thus triggering protein kinase A activation and tyrosine phosphorylation (Herrero et al 2000) and is also involved in activation of protein ERKs (Thundathil et al 2003, O'Flaherty et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

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Nitric oxide (NO), a modulator of several physiological processes, is involved in different human sperm functions. We have investigated whether NO may stimulate the motility of human spermatozoa via activation of the soluble guanylate cyclase (sGC)/cGMP pathway. Sperm samples obtained by masturbation from 70 normozoospermic patients were processed by the swim-up technique. The kinetic parameters of the motile sperm-rich fractions were assessed by computer-assisted sperm analysis. After a 30-90 min incubation, the NO donor S-nitrosoglutathione (GSNO) exerted a significant enhancing effect on progressive motility (77, 78, and 78% vs 66, 65, and 62% of the control at the corresponding time), straight linear velocity (44, 49, and 48 mm/s vs 34, 35, and 35.5 mm/s), curvilinear velocity (81, 83, and 84 mm/s vs 68 mm/s), and average path velocity (52, 57, and 54 mm/s vs 40, 42, and 42 mm/s) at 5 mM but not at lower concentrations, and in parallel increased the synthesis of cGMP. A similar effect was obtained with the NO donor spermine NONOate after 30 and 60 min. The GSNO-induced effects on sperm motility were abolished by 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (a specific sGC inhibitor) and mimicked by 8-bromo-cGMP (8-Br-cGMP; a cell-permeating cGMP analog); the treatment with Rp-8-Br-cGMPS (an inhibitor of cGMP-dependent protein kinases) prevented both the GSNO-and the 8-Br-cGMP-induced responses. On the contrary, we did not observe any effect of the cGMP/PRKG1 (PKG) pathway modulators on the onset of hyperactivated sperm motility. Our results suggest that NO stimulates human sperm motility via the activation of sGC, the subsequent synthesis of cGMP, and the activation of cGMP-dependent protein kinases.

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Section: Introductionmentioning

confidence: 99%

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Miraglia¹,

Angelis²,

Gazzano³

et al. 2011

Reproduction

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“…On the other hand, excessive NOS may induce the production of large amounts of NO metabolites in response to a variety of stressors, possibly reducing the survival rate and motility of sperm cells [18,20]. High levels of NO may also reduce testosterone secretion, as indicated by the suppression of testosterone secretion in male rats treated with the NO donor, isosorbide dinitrate (ISDN) [1].…”

mentioning

confidence: 99%

Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

Ha¹,

Kim

Shin

2004

The Journal of Veterinary Medical Science

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ABSTRACT. The expression of three isoforms of nitric oxide synthase (NOS) were examined in the testis and epididymis of a thoroughbred horse. Immunohistochemical studies demonstrated the presence of eNOS immunostaining in some germ cells in the seminiferous tubules and in vascular endothelial cells in the interstitial tissues. Interstitial cells, most likely Leydig cells, were also intensel y immunopositive for eNOS. The pattern of immunostaining for nNOS was similar to that for eNOS in the testis. Weak expression of iNOS was detec ted in the seminiferous tubules of the testis, but intense expression was found in interstitial cells. Inducible NOS was also stron gly detected in stereocilia, sperm, epithelium and connective tissue of the epididymis of normal horses. These findings suggest that three isoforms of NOS are expressed in the testis and epididymis of horse and that they play important roles in the biology of interstitial cells that produce testosterone, as well as in spermatogenesis in the seminiferous tubules. KEY WORDS: epididymis, equine, nitric oxide synthase, testis.

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“…Estudos recentes mostraram que entre 40% e 88% dos homens com baixa fertilidade apresentam elevados níveis de EOR no plasma seminal (15 (24). Em situações de falha na regulação da ONS, ocorre uma excessiva produção de ON, que pode causar patologias aos espermatozóides, diminuindo a fertilidade (25).…”

Section: Oxidação Biológicaunclassified

Efeito oxidativo do óxido nítrico e infertilidade no macho

Ferreira¹,

Silveira²,

Freneau³

2002

J. Bras. Patol. Med. Lab.

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Andrology: Effects of nitric oxide on human spermatozoa: evidence that nitric oxide decreases sperm motility and induces sperm toxicity

Cited by 199 publications

References 19 publications

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Nitric oxide stimulates human sperm motility via activation of the cyclic GMP/protein kinase G signaling pathway

Expression of Constitutive Endothelial, Neuronal and Inducible Nitric Oxide Synthase in the Testis and Epididymis of Horse

Efeito oxidativo do óxido nítrico e infertilidade no macho

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