Andrographolide acts through inhibition of ERK1/2 and Akt phosphorylation to suppress chemotactic migration

Tsai, Hwei-Ru; Yang, Liming; Tsai, Wei‐Jern; Chiou, Wen-Fei

doi:10.1016/j.ejphar.2004.07.077

Cited by 78 publications

(66 citation statements)

References 37 publications

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“…The inhibition of the release of these cytokines by andrographolide was compared to the synthetic glucocorticoid dexamethasone, showing andrographolide to have a similar effect as dexamethasone, but with a lower potency [24,52]. Also, the effect of andrographolide on the cellular chemotaxis, a response that allows the movement of inflammatory cells to the injured tissue, show that it reduces the chemotactic migration of macrophage induced by C5a, which may contribute to its anti-inflammatory activity [53]. In local or systemic inflammatory disorders there is an enhanced formation of nitric oxide (NO) following the expression of inducible nitric oxide synthase (iNOS).…”

Section: In Vitro Studiesmentioning

confidence: 99%

Andrographolide a New Potential Drug for the Long Term Treatment of Rheumatoid Arthritis Disease

Hidalgo¹,

Hancke²,

Bertoglio³

et al. 2013

Innovative Rheumatology

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Section: In Vitro Studiesmentioning

confidence: 99%

Andrographolide a New Potential Drug for the Long Term Treatment of Rheumatoid Arthritis Disease

Hidalgo¹,

Hancke²,

Bertoglio³

et al. 2013

Innovative Rheumatology

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“…Reintroduction of a full-length MPL1 into mpl1 ؊ cells restored aggregation, ERK2 regulation, random and directional motility, and cAMP production similar to wild-type cells. We propose that MPL1 is a novel phosphatase essential for proper regulation of ERK2 phosphorylation and optimal motility during development.Mitogen-activated protein kinases (MAPKs) are central in the regulation of proliferation, differentiation, and cell migration in diverse eukaryotic cells (10,13,21,25,26,27). The MAP kinase ERK2 also plays critical roles during Dictyostelium development.…”

mentioning

confidence: 99%

“…Mitogen-activated protein kinases (MAPKs) are central in the regulation of proliferation, differentiation, and cell migration in diverse eukaryotic cells (10,13,21,25,26,27). The MAP kinase ERK2 also plays critical roles during Dictyostelium development.…”

mentioning

confidence: 99%

MPL1, a Novel Phosphatase with Leucine-Rich Repeats, Is Essential for Proper ERK2 Phosphorylation and Cell Motility

et al. 2008

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The novel Dictyostelium phosphatase MPL1 contains six leucine-rich repeats at the amino-terminal end and a phosphatase domain at the carboxyl end. Similarly architectured phosphatases exist among other protozoa, such as Entamoeba histolytica, Leishmania major, and Trypanosoma cruzi. MPL1 was strongly induced after 5 h of development; ablation by homologous recombination led to defective streaming and aggregation during development. In addition, cyclic AMP (cAMP)-pulsed mpl1 ؊ cells showed reduced random and directional motility. At the molecular level, mpl1؊ cells displayed higher prestimulus and persistent poststimulus ERK2 phosphorylation in response to cAMP stimulation. Consistent with their phenotype of persistent ERK2 phosphorylation, mpl1؊ cells also displayed an aberrant pattern of cAMP production, resembling that of the regA ؊ cells. Reintroduction of a full-length MPL1 into mpl1 ؊ cells restored aggregation, ERK2 regulation, random and directional motility, and cAMP production similar to wild-type cells. We propose that MPL1 is a novel phosphatase essential for proper regulation of ERK2 phosphorylation and optimal motility during development.Mitogen-activated protein kinases (MAPKs) are central in the regulation of proliferation, differentiation, and cell migration in diverse eukaryotic cells (10,13,21,25,26,27). The MAP kinase ERK2 also plays critical roles during Dictyostelium development. ERK2 is essential for initiation and propagation of periodic cyclic AMP (cAMP) pulses during aggregation and differentiation. Chemoattractants, such as cAMP, induce ERK2 activation. Activated ERK2 subsequently inhibits the intracellular cAMP-specific phosphodiesterase RegA, resulting in an increase in the cytosolic cAMP level (15,19,22). erk2 Ϫ cells, starved for 8 h, exhibited a decrease in motility and a severe chemotaxis defect toward a cAMP gradient. Aberrancy in chemotaxis was aggravated in the presence of a strong cAMP gradient (2 M) compared to a weak one (0.1 M) (27). erk2 Ϫ cells also display defective cytoskeletal remodeling in response to chemoattractant stimulation. A polarized wild-type cell typically displays a single dominant leading edge enriched with F-actin. Myosin II localizes to the lateral side and back of a polarized cell, where it functions to suppress lateral pseudopods and provides tractional force to the back. This single dominant leading edge disintegrates but forms again after 7 min in response to global cAMP stimulation (27). In contrast, erk2 Ϫ cells, under the same condition, displayed multiple crown-like membranous protrusions, which were enriched not only in F-actin but also in myosin II (27). This aberrant structure, which was proposed to be less stable and unable to provide necessary traction force for cells to move, is believed to be the reason why erk2 Ϫ cells are less motile than wild-type cells (27).It is well-established that the dual phosphorylation of threonine 176 and tyrosine 178 residues of the ERK2 activation loop, often called the TEY motif, mediates activation of ERK2...

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“…12 The aqueous extract of Andrographis paniculata reduced oxidative stress in lymphocytes isolated from rat when exposed to nicotine. 13 Andrographolide and 14-deoxy-11,12-didehydroandrographolide drastically inhibited thrombin-induced aggragation of platelet while neoandrographolide has little or no activity.…”

mentioning

confidence: 99%

Hematological Toxicity in Rats; Therapeutic Properties of Andrographis Paniculata on Oxaliplatin Induced

Ain

Bano

Beg

et al. 2017

TPMJ

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Andrographolide acts through inhibition of ERK1/2 and Akt phosphorylation to suppress chemotactic migration

Cited by 78 publications

References 37 publications

Andrographolide a New Potential Drug for the Long Term Treatment of Rheumatoid Arthritis Disease

Andrographolide a New Potential Drug for the Long Term Treatment of Rheumatoid Arthritis Disease

MPL1, a Novel Phosphatase with Leucine-Rich Repeats, Is Essential for Proper ERK2 Phosphorylation and Cell Motility

Hematological Toxicity in Rats; Therapeutic Properties of Andrographis Paniculata on Oxaliplatin Induced

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