Androgens decrease and retinoids increase the expression of insulin-like growth factor-binding protein-3 in LNCaP prostatic adenocarcinoma cells

Goossens, Karine; Esquenet, Murielle; Swinnen, Johannes V.; Manin, M.; Rombauts, Wilfried; Verhoeven, Guido

doi:10.1016/s0303-7207(99)00122-7

Cited by 34 publications

(23 citation statements)

References 32 publications

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“…The increases in IGF-I and IGF-IR were accompanied by a decrease in IGFBP-3; this latter finding has been reported previously (25). Here again, the effects of DHT and T were greater than those of DHEA and E 2 .…”

Section: Discussionsupporting

confidence: 89%

Comparative effects of DHEA vs. testosterone, dihydrotestosterone, and estradiol on proliferation and gene expression in human LNCaP prostate cancer cells

Arnold¹,

Le²,

McFann³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

100

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Section: Discussionsupporting

confidence: 89%

Comparative effects of DHEA vs. testosterone, dihydrotestosterone, and estradiol on proliferation and gene expression in human LNCaP prostate cancer cells

Arnold¹,

Le²,

McFann³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

100

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“…Other experimental prostate models of androgen regulation of stromal-epithelial signaling [35][36][37][38] or intracrine production of DHEA metabolites (ERβ ligand 7α-hydroxy DHEA) [22] have been described. The coculture model presented herein also provides an opportunity to elucidate the role of the tumor microenvironment in cancer progression by comparing responses of epithelial cells to normal vs. reactive stromal cells.…”

Section: Discussionmentioning

confidence: 99%

Human prostate stromal cells stimulate increased PSA production in DHEA-treated prostate cancer epithelial cells

Arnold

Gray

Jacobowitz

et al. 2008

The Journal of Steroid Biochemistry and Molecular Biology

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Dehydroepiandrosterone (DHEA) is commonly used as a dietary supplement and may affect prostate pathophysiology when metabolized to androgens and/or estrogens. Human prostate LAPC-4 cancer cells with a wild type androgen receptor (AR), were treated with DHEA, androgens (DHT, T, or R1881), and E 2 and assayed for PSA protein and gene expression. In LAPC-4 monocultures, DHEA and E 2 induced little or no increase in PSA protein or mRNA expression compared to androgentreated cells. When prostate cancer-associated (6S) stromal cells were added in coculture, DHEA stimulated LAPC-4 cell PSA protein secretion to levels approaching induction by DHT. Also, DHEA induced 15-fold more PSA mRNA in LAPC-4 cocultures than in monocultures. LAPC-4 proliferation was increased 2-3 fold when cocultured with 6S stromal cells regardless of hormone treatment. DHEA-treated 6S stromal cells exhibited a dose-and time-dependent increase in T secretion, demonstrating stromal cell metabolism of DHEA to T. Coculture with non-cancerous stroma did not induce LAPC-4 PSA production, suggesting a differential modulation of DHEA effect in a cancerassociated prostate stromal environment. This coculture model provides a research approach to reveal detailed endocrine, intracrine, and paracrine signaling between stromal and epithelial cells that regulate tissue homeostasis within the prostate, and the role of the tumor microenvironment in cancer progression.

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“…The role of the insulin-like growth factor binding proteins (IGFBP), especially IGFBP-3, can be growth inhibitory or stimulatory (Schmid et al 1991, Oh et al 1995, Angelloz-Nicoud et al 1996, Rajah et al 1997. Several studies have demonstrated that IGFBP-3 expression increases in response to 1,25-(OH) 2 D 3 , or its analogs, in established prostate cancer cell lines (Huynh et al 1996, Nickerson et al 1998, Goossens et al 1999, Nickerson & Huynh 1999. Most studies have correlated the increase in IGFBP-3 with a decrease in cell growth but none have determined if the 1,25-(OH) 2 D 3 -induced increase in IGFBP-3 alters the response of the cells to IGF ligands.…”

Section: Introductionmentioning

confidence: 99%

Regulation of proliferation of prostate epithelial cells by 1,25-dihydroxyvitamin D3 is accompanied by an increase in insulin-like growth factor binding protein-3

Sprenger

Peterson²,

Lance³

et al. 2001

Journal of Endocrinology

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The biologically active form of vitamin D, 1,25-dihydroxyvitamin D 3 (1,25-(OH) 2 D 3 ) has been shown to regulate the proliferation of human prostate epithelial cell lines. Since the insulin-like growth factor (IGF) system is involved in the transformation process of epithelial cells, the following study was undertaken to determine if the IGF system, in particular IGF binding protein-3 (IGFBP-3), is altered by 1,25-(OH) 2 D 3 in normal prostate epithelial cells as part of a mechanism for inhibition of transformation. Two cell systems were used in this study: Northern blots demonstrated an increase in mRNA for IGFBP-3. Proliferation assays using an antibody designed to block the IGF-independent effects of IGFBP-3 failed to reverse the inhibitory effect of 1,25-(OH) 2 D 3 . Thus, IGFBP-3 acts in an IGF-dependent manner to inhibit cell growth of benign prostate epithelial cells.

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Androgens decrease and retinoids increase the expression of insulin-like growth factor-binding protein-3 in LNCaP prostatic adenocarcinoma cells

Cited by 34 publications

References 32 publications

Comparative effects of DHEA vs. testosterone, dihydrotestosterone, and estradiol on proliferation and gene expression in human LNCaP prostate cancer cells

Comparative effects of DHEA vs. testosterone, dihydrotestosterone, and estradiol on proliferation and gene expression in human LNCaP prostate cancer cells

Human prostate stromal cells stimulate increased PSA production in DHEA-treated prostate cancer epithelial cells

Regulation of proliferation of prostate epithelial cells by 1,25-dihydroxyvitamin D3 is accompanied by an increase in insulin-like growth factor binding protein-3

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