Androgens binding proteins in the cytosol from prepubertal male rat hypothalamus, preoptic area and brain cortex

Hannouche, N; Thieulant, Marie-Lise; Samperez, S; Jouan, P

doi:10.1016/0022-4731(78)90076-6

Cited by 12 publications

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This apparent lack of cytosolic dependent nuclear uptake and retention of " Most studies used 100-and 1000-fold unlabeled competitor. 6 Only one laboratory reporting (55).…”

Section: Biochemical Studies Of Androgen Receptors In the Brainmentioning

confidence: 97%

Androgen Receptors in the Brain: What Are We Measuring?*

Sheridan

1983

Endocrine Reviews

View full text Add to dashboard Cite

Androgen has long been known to act on the brain to modify behavior and other brain functions. In the past, two methods have been used to characterize the putative receptors which mediate these actions. Autoradiography has been used to map and identify androgen binding neurons. Binding studies have been conducted to quantify and characterize the system(s). The resultant data are discordant and a new model is proposed to resolve the apparent differences. It is proposed that there are three categories of receptors for androgen in the brain. One receptor preferentially binds testosterone and a second one preferentially binds DHT. Both of these receptors are in equilibrium between nucleus and cytoplasm according to the free water content of the compartments. Both of these receptors can be activated and transformed by steroid and thus concentrate in the nucleus. It is proposed that a third receptor binds both steroids with the same relative affinity. However, this third receptor can only be activated but not transformed (i.e. it does not concentrate in the nucleus). The proposed system implies that testosterone acts on a few discrete populations of neurons in the brain while DHT has a very diffuse action on the central nervous system.

show abstract

“…This apparent lack of cytosolic dependent nuclear uptake and retention of " Most studies used 100-and 1000-fold unlabeled competitor. 6 Only one laboratory reporting (55).…”

Section: Biochemical Studies Of Androgen Receptors In the Brainmentioning

confidence: 97%

Androgen Receptors in the Brain: What Are We Measuring?*

Sheridan

1983

Endocrine Reviews

View full text Add to dashboard Cite

show abstract

Estrogen/progesterone treatment in adulthood affects the size of several components of the medial preoptic area in the male rat

Bloch¹,

Gorski²

1988

J of Comparative Neurology

View full text Add to dashboard Cite

The results of preliminary studies suggested that steroid and/or propylthiouracil (PTU) treatment of adult gonadectomized (Gxd) male rats significantly reduced the volume of the sexually dimorphic nucleus of the preoptic area (SDN-POA). Therefore, we designed a study to examine this effect in detail. Groups of adult rats were sham Gxd (intact) or Gxd, then treated with multiple injections of oil (males and females), or estrogen and progesterone (males). Gonadectomized estrogen/progesterone-treated males had a significantly smaller SDN-POA volume, smaller volume of the medial division of the medial preoptic nucleus (MPNm), smaller volume of the anteroventral MPNm (MPNav), and larger volume of the anteroventral periventricular nucleus (AVPv). The volume of the central division of the medial preoptic nucleus (MPNc) or of the suprachiasmatic nucleus was not affected. There were no differences between Gxd estrogen/progesterone-treated males vs the group that received PTU as well, indicating that the PTU treatment was unnecessary. The reduced volume of the SDN-POA was due to a reduced volume of the MPNav and of the portion of the SDN-POA located within the MPNm-exclusive of the MPNav and MPNc. In conclusion, estrogen/progesterone treatment in adulthood caused significant changes in the volume of several medial preoptic structures in two separate groups of Gxd males. Because the steroids produced no significant effects in intact males, testicular hormones appear to "protect" these structures from the effects of the estrogen/progesterone treatment.

show abstract