Androgens and androgen receptor signaling contribute to ovarian development in the chicken embryo

Tanaka, Ryoma; Izumi, Hiroyuki; Kuroiwa, Asato

doi:10.1016/j.mce.2017.01.008

Cited by 11 publications

(10 citation statements)

References 20 publications

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“…The androgen receptor (AR) is a single-copy gene on the X chromosome and a ligand-dependent transcription factor that belongs to the steroid hormone receptor family and plays a crucial role in the pathogenesis of infertility. 4,5 The level of AR is regulated by steroid ligand endogenous testosterone (T), which is based on the transcriptional regulatory function of AR. 6 It has been demonstrated that AR expression is significantly decreased in stress-induced rats, which may result in reduction of T, disrupt AR signaling, and induce cell apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH₂-terminal kinase signaling pathways in rats

Yao

Wang

et al. 2018

Hum Exp Toxicol

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Thyroid hormone deficiency can impair testicular function. However, knowledge of the effects of mitogen-activated protein kinase (MAPK) pathways on testicular mitochondrial oxidative damage induced by hypothyroidism is still rudimentary. This study aims to explore the possible mechanisms of testicular mitochondrial oxidative damage in hypothyroidism rats. Wistar male rats were randomly divided into control (C), low- (L), and high-hypothyroidism (H) groups (1 ml/100 g body weights (BWs)/day 0, 0.001% and 0.1% propylthiouracil, respectively) by intragastric gavage for 60 days. Blood samples were collected to measure the levels of serum triiodothyronine (T3), thyroxine (T4), and thyroid stimulating hormone (TSH). Testicular mitochondrial homogenates were used to measure the activities of superoxide dismutase (SOD), catalase (CAT), and Ca2+-ATPase as well as protein and mRNA expression of androgen receptor (AR), p38 MAPK, and c-Jun NH2-terminal kinase (JNK). Results showed that the BWs, testes weights, and levels of T3 and T4 were all significantly decreased and the testes coefficient and level of TSH were significantly increased in the H group. There were significant decreases in SOD activity in the H group as well as decreases in CAT and Ca2+-ATPase activities in the L and H groups. Additionally, protein expression of AR decreased significantly and protein expression of phosphorylated p38MAPK and JNK increased significantly in the H group. Therefore, the study suggests that hypothyroidism could affect male reproductive function by disturbing expression of AR, changing the activity of Ca2+-ATPase, inducing oxidative stress and then leading to activation of p38MAPK and JNK signaling in the testicular mitochondria.

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Section: Introductionmentioning

confidence: 99%

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH₂-terminal kinase signaling pathways in rats

Yao

Wang

et al. 2018

Hum Exp Toxicol

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“…Three affected genes, HUWE 1, PHF 8 and KLF 8, are related to breast or ovary cancers [ 43 – 45 ]. Androgen receptor ( AR ) gene is critical for the ovarian development [ 46 ]. ZC 3 H 12 B gene is negative regulator in macrophage activation and may involve in host immunity and inflammatory diseases [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Detection of genomic structural variations in Guizhou indigenous pigs and the comparison with other breeds

et al. 2018

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Genomic structural variation (SV) is noticed for the contribution to genetic diversity and phenotypic changes. Guizhou indigenous pig (GZP) has been raised for hundreds of years with many special characteristics. The present paper aimed to uncover the influence of SV on gene polymorphism and the genetic mechanisms of phenotypic traits for GZP. Eighteen GZPs were chosen for resequencing by Illumina sequencing platform. The confident SVs of GZP were called out by both programs of pindel and softSV simultaneously and compared with the SVs deduced from the genomic data of European pig (EUP) and the native pig outside of Guizhou, China (NPOG). A total of 39,166 SVs were detected and covered 27.37 Mb of pig genome. All of 76 SVs were confirmed in GZP pig population by PCR method. The SVs numbers in NPOG and GZP were about 1.8 to 1.9 times higher than that in EUP. And a SV hotspot was found out from the 20 Mb of chromosome X of GZP, which harbored 29 genes and focused on histone modification. More than half of SVs was positioned in the intergenic regions and about one third of SVs in the introns of genes. And we found that SVs tended to locate in genes produced multi-transcripts, in which a positive correlation was found out between the numbers of SV and the gene transcripts. It illustrated that the primary mode of SVs might function on the regulation of gene expression or the transcripts splicing process. A total of 1,628 protein-coding genes were disturbed by 1,956 SVs specific in GZP, in which 93 GZP-specific SV-related genes would lose their functions due to the SV interference and gathered in reproduction ability. Interestingly, the 1,628 protein-coding genes were mainly enriched in estrogen receptor binding, steroid hormone receptor binding, retinoic acid receptor binding, oxytocin signaling pathway, mTOR signaling pathway, axon guidance and cholinergic synapse pathways. It suggested that SV might be a reason for the strong adaptability and low fecundity of GZP, and 51 candidate genes would be useful for the configuration phenotype in Xiang pig breed.

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“…Two miRNAs targeting different regions of chicken DDX4 mRNA and two non-targeting (scrambled) controls were designed using BLOCK-iT RNAi Designer (Thermo Fisher Scientific, MA) and siRNA Wizard v3.1 (Thermo Fisher Scientific) ( Table 1). The miRNA constructs were cloned as described previously (Tanaka et al, 2017). pRFPRNAi.C and RCASRNAi were 6 provided by ARK-Genomics, The Roslin Institute (Das et al, 2006…”

Section: Construction Of Rnai Vectorsmentioning

confidence: 99%

Knockdown of DEAD-box helicase 4 (DDX4) decreases the number of germ cells in male and female chicken embryonic gonads

Aduma

Izumi

Mizushima

et al. 2019

Reprod. Fertil. Dev.

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DEAD-box helicase 4 (DDX4; also known as vasa) is essential for the proper formation and maintenance of germ cells. Although DDX4 is conserved in a variety of vertebrates and invertebrates, its roles differ between species. This study investigated the function of DDX4 in chicken embryos by knocking down its expression using retroviral vectors that encoded DDX4-targeting microRNAs. DDX4 was effectively depleted invitro and invivo via this approach. Male and female gonads of DDX4-knockdown embryos contained a decreased number of primordial germ cells, indicating that DDX4 is essential to maintain a normal level of these cells in chicken embryos of both sexes. Expression of doublesex and mab-3 related transcription factor 1 (DMRT1) and sex determining region Y-box 9 (SOX9), which are involved in testis determination and differentiation, was normal in male gonads of DDX4-knockdown embryos. In contrast, expression of cytochrome P450 family 19 subfamily A member 1 (CYP19A1), which encodes aromatase and is essential for ovary development, was significantly decreased in female gonads of DDX4-knockdown embryos. Expression of forkhead box L2 (FOXL2), which plays an important role in ovary differentiation, was also slightly reduced in DDX4-knockdown embryos, but not significantly. Based on several pieces of evidence FOXL2 was hypothesised to regulate aromatase expression. The results of this study indicate that aromatase expression is also regulated by several additional pathways.

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Androgens and androgen receptor signaling contribute to ovarian development in the chicken embryo

Cited by 11 publications

References 20 publications

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH₂-terminal kinase signaling pathways in rats

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH₂-terminal kinase signaling pathways in rats

Detection of genomic structural variations in Guizhou indigenous pigs and the comparison with other breeds

Knockdown of DEAD-box helicase 4 (DDX4) decreases the number of germ cells in male and female chicken embryonic gonads

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Androgens and androgen receptor signaling contribute to ovarian development in the chicken embryo

Cited by 11 publications

References 20 publications

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH2-terminal kinase signaling pathways in rats

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH2-terminal kinase signaling pathways in rats

Detection of genomic structural variations in Guizhou indigenous pigs and the comparison with other breeds

Knockdown of DEAD-box helicase 4 (DDX4) decreases the number of germ cells in male and female chicken embryonic gonads

Contact Info

Product

Resources

About

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH₂-terminal kinase signaling pathways in rats

Influence of hypothyroidism on testicular mitochondrial oxidative stress by activating the p38 mitogen-activated protein kinase and c-Jun NH₂-terminal kinase signaling pathways in rats