Androgenic potential in coconut (Cocos nucifera L.)

Perera, P. I. P.; Hocher, Valérie; Verdeil, Jean‐Luc; Bandupriya, H.D.D.; Yakandawala, Deepthi; Weerakoon, L.K.

doi:10.1007/s11240-008-9337-5

Cited by 38 publications

(43 citation statements)

References 37 publications

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“…2b, 2c, 2d). This result resembled to embryo and callus formation of pollen grain culture of coconut (Perera et al 2008). Formation of proembryo structures could happen without suspensor (Fig.…”

Section: Calli Cultured In Liquid Mediumsupporting

confidence: 59%

HISTOLOGICAL ANALYSIS OF In Vitro CULTURED COCONUT ENDOSPERM

Sukamto

2017

BIOTROPIA

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Coconut is a very important plant for the livelihood of people in tropical countries. It is also used as an icon of tropical region. Coconut fruit is very heavy and can cause injuries if the fruit falls down and hits somebody who happens to be underneath a coconut tree. In order to avoid the accident, the coconut fruits have to be regularly cut off. Coconut tree originated from in vitro cultured endosperm is a triploid plant that produces seedless fruit (without endocarp). Coconut fruit without endocarp is not heavy. The objective of this study was to investigate plant regeneration of fresh and in vitro cultured coconut endosperms. The fresh and developed in vitro cultured coconut endosperms were observed using histological analysis. Solid endosperm of seven month-old postanthesis coconut from "Samoan Dwarf " cultivar was freshly picked up and cultured in vitro on modified Branton & Blake formula. Histological study of fresh coconut endosperm showed that the endosperm consisted of parenchyma cells, which were relatively uniform in shape and size, with some nuclei consisted of 1 -5 nucleoli. Three month-old calli of in vitro grown coconut endosperm in semi solid media showed that its cells varied in shape and size, characterized by high nucleus to cytoplasm ratio, high starch, protein and lipid contents which underwent many divisions. Seven month-old calli of in vitro grown coconut endosperm in liquid media showed embryogenic cells which resembled proembryos. Fourteen month-old bud-like structure of coconut endosperm in semi solid media showed a meristematic layer, tunica-corpus structure, cortex-like region and tracheids of xylem. These results indicated that the bud-like structure was an early stage of shoot bud formation in coconut endosperm. This is the first report of early stage of shoot bud formation occurring on coconut endosperm cultured in vitro.

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Section: Calli Cultured In Liquid Mediumsupporting

confidence: 59%

HISTOLOGICAL ANALYSIS OF In Vitro CULTURED COCONUT ENDOSPERM

Sukamto

2017

BIOTROPIA

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“…The activated charcoal (AC) in the maturation medium can adsorb inhibitory substances such as phenolic compounds, ethylene or auxin residues that could prevent the development of somatic embryos (Pereira et al 2008, Aboshama 2011. In a study investigating the maturation of somatic embryos of different varieties of grape, López-Pérez et al (2005) found that the use of a medium supplemented with AC (2.5 g L -1 ) produced a significantly higher maturation rate (99.5%) than the medium without AC (5.8%).…”

Section: Maturation Of Somatic Embryosmentioning

confidence: 99%

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Cipriano

Cruz

Mancini

et al. 2018

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate somatic embryogenesis in juvenile explants of the THB papaya cultivar. Apical shoots and cotyledonary leaves were inoculated in an induction medium composed of different concentrations of 2,4-D (6, 9, 12, 15 and 18 µM) or 4-CPA (19, 22, 25, 28 and 31 µM). The embryogenic calluses were transferred to a maturation medium for 30 days. Histological analysis were done during the induction and scanning electron microscopy after maturing. For both types of auxin, embryogenesis was achieved at higher frequencies with cotyledonary leaves incubated in induction medium than with apical shoots; except for callogenesis. The early-stage embryos (e.g., globular or heartshape) predominated. Among the auxins, best results were observed in cotyledonary leaves induced with 4-CPA (25 µM). Histological analyses of the cotyledonary leaf-derived calluses confirmed that the somatic embryos (SEs) formed from parenchyma cells, predominantly differentiated via indirect and multicellular origin and infrequently via synchronized embryogenesis. The secondary embryogenesis was observed during induction and maturation phases in papaya THB cultivar. The combination of ABA (0.5 µM) and AC (15 g L -1 ) in maturation medium resulted in the highest somatic embryogenesis induction frequency (70 SEs callus -1 ) and the lowest percentage of early germination (4%).

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“…In those early studies, neither ploidy level determination nor plantlet regeneration was reported. However, in a more recent series of studies it has been reported that somatic embryo structures, with root and shoot apices, have been produced through anther culture (Perera et al 2007a(Perera et al , 2008a, and finally homozygotic plants (Perera et al 2008b). The basic procedures now used employ a culture medium developed by Karunaratne and Periyapperuma (1989) and supplemented with a high concentration of sucrose (9 %) (Perera et al 2008a(Perera et al , 2009c.…”

Section: Homozygote Production Via Anther Culturementioning

confidence: 99%

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Nguyen

Bandupriya

López‐Villalobos

et al. 2015

Planta

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The present review discusses not only advances in coconut tissue culture and associated biotechnological interventions but also future research directions toward the resilience of this important palm crop. Coconut (Cocos nucifera L.) is commonly known as the 'tree of life'. Every component of the palm can be used to produce items of value and many can be converted into industrial products. Coconut cultivation faces a number of acute problems that reduce its productivity and competitiveness. These problems include various biotic and abiotic challenges as well as an unstable market for its traditional oil-based products. Around 10 million small-holder farmers cultivate coconut palms worldwide on c. 12 million hectares of land, and many more people own a few coconut palms that contribute to their livelihoods. Inefficiency in the production of seedlings for replanting remains an issue; however, tissue culture and other biotechnological interventions are expected to provide pragmatic solutions. Over the past 60 years, much research has been directed towards developing and improving protocols for (i) embryo culture; (ii) clonal propagation via somatic embryogenesis; (iii) homozygote production via anther culture; (iv) germplasm conservation via cryopreservation; and (v) genetic transformation. Recently other advances have revealed possible new ways to improve these protocols. Although effective embryo culture and cryopreservation are now possible, the limited frequency of conversion of somatic embryos to ex vitro seedlings still prevents the large-scale clonal propagation of coconut. This review illustrates how our knowledge of tissue culture and associated biotechnological interventions in coconut has so far developed. Further improvement of protocols and their application to a wider range of germplasm will continue to open up new horizons for the collection, conservation, breeding and productivity of coconut.

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Androgenic potential in coconut (Cocos nucifera L.)

Cited by 38 publications

References 37 publications

HISTOLOGICAL ANALYSIS OF In Vitro CULTURED COCONUT ENDOSPERM

HISTOLOGICAL ANALYSIS OF In Vitro CULTURED COCONUT ENDOSPERM

Somatic embryogenesis in Carica papaya as affected by auxins and explants, and morphoanatomical-related aspects

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

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