Androgenic and antiandrogenic activities in water and sediment samples from the river Lambro, Italy, detected by yeast androgen screen and chemical analyses

Urbatzka, Ralph; Cauwenberge, Anne van; Maggioni, Silvia; Viganò, Luigi; Mandich, Alberta; Benfenati, Emilio; Lutz, Ilka; Kloas, Werner

doi:10.1016/j.chemosphere.2006.11.041

Cited by 113 publications

(56 citation statements)

References 36 publications

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“…The androgenic potency in Swedish domestic STP effluents varied from not detected to 160 ng DEQ/L [38], which is slightly higher than the potencies in the four STP effluents from the present study. Androgenic activity was less frequently detected in surface water and sediment of the Pearl River system, which is consistent with the results of some previous studies [13,39,40]. The possible explanation for the lower androgenic activity is that the agonistic androgenic potency might be masked by androgen receptor antagonists in the extracts [12,40].…”

Section: Discussionsupporting

confidence: 91%

“…The possible explanation for the lower androgenic activity is that the agonistic androgenic potency might be masked by androgen receptor antagonists in the extracts [12,40]. Hence, antiandrogenic activity is usually prevalent in various environmental matrices as demonstrated in the present and previous studies [39,40]. Another reason for prevalent estrogenic and antiandrogenic activities in the environment could be the wide presence of estrogenic chemicals in surface waters [6,28] and some xenoestrogen chemicals such as bisphenol A and o,p 0 -DDT (o,p 0 -1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane) could display both estrogenic activity and antiandrogenic activity in the environment [15].…”

Section: Discussionsupporting

confidence: 52%

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Screening of multiple hormonal activities in surface water and sediment from the Pearl River system, South China, using effect‐directed in vitro bioassays

Zhao

Ying

Yang

et al. 2011

Enviro Toxic and Chemistry

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Abstract-This paper reports screening of multiple hormonal activities (estrogenic and androgenic activities, antiestrogenic and antiandrogenic activities) for surface water and sediment from the Pearl River system (Liuxi, Zhujiang, and Shijing rivers) in South China, using in vitro recombinant yeast bioassays. The detection frequencies for estrogenic and antiandrogenic activities were both 100% in surface water and 81 and 93% in sediment, respectively. The levels of estrogenic activity were 0.23 to 324 ng 17b-estradiol equivalent concentration (EEQ)/L in surface water and 0 to 101 ng EEQ/g in sediment. Antiandrogenic activities were in the range of 20.4 to 935 Â 10 3 ng flutamide equivalent concentration (FEQ)/L in surface water and 0 to 154 Â 10 3 ng FEQ/g in sediment. Moreover, estrogenic activity and antiandrogenic activity in sediment showed good correlation (R 2 ¼ 0.7187), suggesting that the agonists of estrogen receptor and the antagonists of androgen receptor co-occurred in sediment. The detection frequencies for androgenic and antiestrogenic activities were 41 and 29% in surface water and 61 and 4% in sediment, respectively. The levels of androgenic activities were 0 to 45.4 ng dihydrotestosterone equivalent concentration (DEQ)/L in surface water, and the potency was very weak in the only detected sediment site. The levels of antiestrogenic activity were 0 to 1,296 Â 10 3 ng tamoxifen equivalent concentration (TEQ)/L in surface water and 0 to 89.5 Â 10 3 ng TEQ/g in sediment. The Shijing River displayed higher levels of hormonal activities than the Zhujiang and Liuxi rivers, indicating that the Shijing River had been suffering from heavy contamination with endocrine-disrupting chemicals. The equivalent concentrations of hormonal activities in some sites were greater than the lowest-observed-effect concentrations reported in the literature, suggesting potential adverse effects on aquatic organisms. Environ.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 52%

Screening of multiple hormonal activities in surface water and sediment from the Pearl River system, South China, using effect‐directed in vitro bioassays

Zhao

Ying

Yang

et al. 2011

Enviro Toxic and Chemistry

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“…BPA has been found in water, surface waters, ground waters, wastewaters, sediments, sewage sludges, landfill leachates, influents and effluents of wastewater treatment plants, garbage leakages, and even tap water (Bruchet et al, 2014;Focazio et al, 2008;Furhacker et al, 2004;Jardim et al, 2012;Nie et al, 2012;Seyhi et al, 2011Seyhi et al, , 2013Shao et al, 2008;Urbatzka et al, 2007;Vethaak et al, 2005;Yamamoto et al, 2001), revealing the potential risk of public exposure to BPA. For example, the maximum concentrations of BPA were found to be 17.2 mg/L in waste landfill leachates (Yamamoto et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Adsorption of Bisphenol-A byEucalyptusbark/magnetite composite: Modeling the effect of some independent parameters by multiple linear regression

Balcı

Erkurt

2016

Adsorption Science & Technology

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In the present study, Eucalyptus camaldulensis bark/magnetite composite was used for potential application as a low-cost adsorbent for the removal Bisphenol-A. The effects of various independent parameters, contact time, initial Bisphenol-A concentration, temperature, pH, and Eucalyptus camaldulensis bark/magnetite composite dosage on adsorption were investigated. It was found that the adsorption capacity of Eucalyptus camaldulensis bark/magnetite composite increases with the increasing of Bisphenol-A concentration, temperature, and decreasing dosage of Eucalyptus camaldulensis bark/magnetite composite. The adsorption capacity was found to be 290.6 mg/g with 0.1 g Eucalyptus camaldulensis bark/magnetite composite at pH 7 and 50 C. The Freundlich isotherm model described the adsorption process better (R 2 ¼ 0.998) than the Langmuir, Dubinin-Radushkevich, Jovanovic, and Vieth-Sladek isotherm models. According to multiple linear regression analysis, Eucalyptus camaldulensis bark/magnetite composite dosage is the most effective parameter on adsorption capacity at equilibrium and independent variables accounted for 79.4% of the total variability of equilibrium adsorption capacity of Eucalyptus camaldulensis bark/magnetite composite.

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“…We followed the basic protocol described in Sohoni and Sumpter (1998), but with modifications for the yeast antiandrogen screen (anti-YAS) as described in later studies (Urbatzka et al, 2007b;Kolle et al, 2011). In brief, assay medium was prepared by adding 0.5 mℓ CPRG to 50 mℓ of fresh growth medium.…”

Section: Recombinant Yeast Anti-androgen Screen (Anti-yas) Proceduresmentioning

confidence: 99%

“…Chemicals having endocrine-disrupting (ED) effects on the reproductive system can in general be categorised in 1 of 4 mode-of-action (MOA) groups (responses), namely, oestrogenic, anti-oestrogenic, androgenic or anti-androgenic (Behrends et al, 2010;Hoffmann and Kloas, 2010). The majority of EDC studies conducted to date have focused on EDCs modulating the oestrogenic response system in one way or the other (Sohoni and Sumpter, 1998;Urbatzka et al, 2007aUrbatzka et al, & 2007bKloas et al, 2009;Behrends et al, 2010). In contrast to the intensive research focus on manmade chemicals that potentially disrupt normal oestrogenic function, relatively few studies have addressed interaction with the androgenic endocrine systems, either from an agonistic or antagonistic perspective (Urbatzka et al, 2007a).…”

Section: Introductionmentioning

confidence: 99%

The potential anti-androgenic effect of agricultural pesticides used in the Western Cape: In vitro investigation of mixture effects

Archer¹,

Wyk²

2015

WSA

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Although it is known that environmental chemicals can affect the oestrogenic system, far less attention has been paid to chemicals interacting with the androgen receptor (AR). Pesticides, particularly fungicides, have been shown to competitively bind or affect expression of the AR in an inhibiting manner. Few studies have addressed anti-androgenic effects of agrochemical use in South Africa. The aim of this study was to screen for the ability of commonly-used pesticides (mostly fungicides) in Western Cape agricultural areas to alter the binding of an androgen (DHT) to the human AR (hAR) using a recombinant yeast androgen screen (YAS), and also to test the additivity mixture interaction hypothesis when commonly-used pesticides with similar modes of action (MOAs) are exposed in mixture. Fungicides vinclozolin, folpet, procymidone, dimethomorph, fenarimol, mancozeb, and the insecticide chlorpyrifos, all independently antagonised the binding of the androgen dihydrotestosterone (DHT) to the AR in a dose-dependent manner. The fungicide mancozeb was found to be the most potent anti-androgen in the assay. Binary, equimolar mixtures of the pesticides also antagonised the binding of DHT to the AR, but at lower IC 50 concentration potencies relative to their individual counterparts. The mixtures of the majority of the selected pesticides did not conform to the expected additive mixture interaction. Only the mixture between dimethomorph and mancozeb showed an additive mixture response at IC 50 concentrations, and, therefore, revealed a more severe AR antagonistic effect compared to their individual counterparts. This study confirmed that pesticides regularly used in agriculture inhibit the binding of androgens to the AR, but when in mixture do not always conform to the predictive addition mixture response model. Also, high relative potencies of individual chemicals in the assay were suppressed when combined with less potent chemicals, showing that the potent chemicals may not be granted access to bind with the AR when exposed in mixture.

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Androgenic and antiandrogenic activities in water and sediment samples from the river Lambro, Italy, detected by yeast androgen screen and chemical analyses

Cited by 113 publications

References 36 publications

Screening of multiple hormonal activities in surface water and sediment from the Pearl River system, South China, using effect‐directed in vitro bioassays

Screening of multiple hormonal activities in surface water and sediment from the Pearl River system, South China, using effect‐directed in vitro bioassays

Adsorption of Bisphenol-A byEucalyptusbark/magnetite composite: Modeling the effect of some independent parameters by multiple linear regression

The potential anti-androgenic effect of agricultural pesticides used in the Western Cape: In vitro investigation of mixture effects

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