Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids

Belicuas, Pedro Radi; Guimarães, C. T.; Paiva, Luciano Vilela; Duarte, Jair Moura; Maluf, Wilson Roberto; Paiva, Edílson

doi:10.1007/s10681-007-9356-z

Cited by 53 publications

(43 citation statements)

References 23 publications

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“…Differences between the hybrids for the HIR of maize haploids have previously been reported by other authors (Kebede et al, 2011;Wu et al, 2014). According to Belicuas et al (2007), these rate differences occur due to the variable expressivity of the R1-Navajo gene, which does not produce precise indication of the seeds.…”

Section: Identification Of Haploids Using the R1-navajo Markermentioning

confidence: 68%

“…As described by Belicuas et al (2007), the R1-navajo gene presents variable expressivity, and thus, the marker does not provide a precise indication of the seeds, which allows false haploids to be selected. To avoid this error, Prigge et al (2011) recommended analyzing and separating the haploid seeds after the harvest because the drying stage generates a darker color of the embryo, contributing to erroneous selection.…”

Section: Haploid Identification By Flow Cytometrymentioning

confidence: 99%

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In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE

Couto

Pinho

et al. 2015

Ciênc. agrotec.

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Artificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities of doubled haploid seeds obtained after the self-fertilization processes were analyzed. It was observed that the germplasm influences haploid induction but not the duplication rates of the tested protocols. Protocol 2 was more efficient for the duplication of haploids, in the percentage of self-fertilized plants after duplication, and in the attainment of doubled haploid lines. Moreover, the haploid inducer line KEMS can produce haploids in a tropical climate. Other markers, in addition to the R1-Navajo system, should be used in the selection of haploid seeds.Index terms: R-Navajo; colchicine; induction of maternal haploids; Zea mays. RESUMOA duplicação cromossômica artificial está dentre as etapas mais importantes na obtenção de duplo-haploides em milho. Este estudo objetivou avaliar a capacidade de indução da linhagem indutora KEMS em clima tropical e testar a eficiência do marcador R1-navajo por meio de citometria de fluxo; avaliar dois protocolos de duplicação cromossômica e, analisar o desenvolvimento dos haploides duplicados no campo. Para isso, quatro genótipos (gerações F1 e F2) foram cruzados com a linhagem KEMS. As sementes obtidas foram selecionadas pelo marcador R1-navajo e submetidas a dois protocolos de duplicação cromossômica. A duplicação foi confirmada por meio de citometria de fluxo. As porcentagens de plantas autofecundadas após duplicação foram analisadas, bem como as quantidades de sementes duplo-haploides obtidas após as autofecundações. Foi observado que o germoplasma influencia a indução de haploides, mas não na taxa de duplicação dos protocolos testados. O protocolo 2 foi mais eficiente na duplicação de haploides, na porcentagem de plantas autofecundadas após duplicação, e na obtenção de linhagens duplo-haploides. Além disso, a linhagem indutora KEMS pode induzir haploides em clima tropical. Outros marcadores além do sistema do R1-navajo devem ser utilizados na seleção de sementes haploides.Termos para indexação: R-navajo; colchicina; indução de haploides maternos; Zea mays.

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Section: Identification Of Haploids Using the R1-navajo Markermentioning

confidence: 68%

Section: Haploid Identification By Flow Cytometrymentioning

confidence: 99%

In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE

Couto

Pinho

et al. 2015

Ciênc. agrotec.

View full text Add to dashboard Cite

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“…Belicuas et al (2007) and Couto et al (2013) used microsatellite markers to detect androgenetic haploids in maize and observed only one band in the haploid individual. Battistelli et al (2013) used SSR to confirm whether the plants that presented peaks for haploid cells in the histograms generated by flow cytometry were truly haploids.…”

Section: Discussionmentioning

confidence: 99%

“…Because they are codominant and stable, these markers allow for the separation of homozygous and heterozygous individuals, enabling the study of genetic inheritance and gene introgression (Belicuas et al, 2007, Li et al, 2009Battistelli et al, 2013;Couto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Verification and characterization of chromosome duplication in haploid maize

Couto¹,

Pinho²,

Pinho³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, and flow cytometry techniques. Evaluated material was obtained using two different chromosome duplication protocols in maize seeds considered haploids, resulting from the cross between the haploid inducer line KEMS and 4 hybrids (GNS 3225, GNS 3032, GNS 3264, and DKB 393). Fourteen days after duplication, plant samples were collected and assessed by flow cytometry. Further, the plants were transplanted to a field, and samples were collected for DNA analyses using microsatellite markers. The tassels were collected during anthesis for pollen viability analyses. Haploid, diploid, and mixoploid individuals were detected using 7000 E.G. de Oliveira Couto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6999-7007 (2015) flow cytometry, demonstrating that this technique was efficient for identifying doubled haploids. The microsatellites markers were also efficient for confirming the ploidies preselected by flow cytometry and for identifying homozygous individuals. Pollen viability showed a significant difference between the evaluated ploidies when the Alexander and propionic-carmin stains were used. The viability rates between the plodies analyzed show potential for fertilization.

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“…In addition, the simplified assembly of a haploid genome is the basis for several sequencing and map developing programs (Dunwell 2010;Sato et al 2009). During a backcross process, DH individuals can be obtained at different generations, together with marker-assisted selection, to help fix the introgressed character (Belicuas et al 2007;Toojinda et al 1998). In somatic interspecific hybrids between crop species and their wild relatives, where often sterile and tetraploids individuals are obtained, anther culture can be used to bring back the ploidy level to the diploid status (Rotino et al 2005).…”

Section: Dhs As An Assisting Tool For Plant Breedingmentioning

confidence: 99%

Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant

Sendra¹

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Thesis layout and cover design: Alba Rivas Sendra and André Arôxa Watercolor drawings: Alba Rivas Sendra AcknowledgementsEn primer lugar, quiero dar las gracias especialmente a Jose María, que me ha guiado en este proceso desde el primer momento. Me ha impulsado a crecer y, cuando no era posible, me ha traído un banquito para que pudiera alcanzar siempre más alto. Con tu ayuda me he convertido en una investigadora y todo lo que he aprendido me acompañará para siempre. Aunque a veces ponernos de acuerdo ha sido un reto, eso representa la esencia misma de la investigación y la ciencia, y lo agradezco sinceramente. Los retos siempre nos inspiran, nos obligan a aprender, a mantenernos activos y a tener menos miedo de las soluciones creativas. Y de esto es mejor tener los bolsillos llenos.También quiero expresar mi sincero agradecimiento a Kim Boutilier. Ella me abrió sus puertas el primer día, y las mantiene abiertas desde entonces. No solo aprendí cosas nuevas de los mejores expertos, sino que la estancia en su laboratorio me permitió ver un mundo diferente, encontrar un segundo hogar, sembrar y recoger muchísimo amor expatriado increíblemente verdadero, y darme cuenta de que son las cosas más diminutas (como puede ser una pequeña bici rosa) las que llenan nuestras vidas de felicidad.Familia, amigos, amigos que se convierten en familia, todos habéis tenido un papel en la parte de mí que queda reflejada en este libro. Los que siempre habéis caminado a mi lado, los que aparecisteis un día y ya nunca os marchasteis, los que acabáis de llegar pero es como si siempre hubieseis estado aquí, los que me dais la bienvenida cuando vuelvo a casa, donde quiera que mi casa esté, e incluso aquellos que solo me acompañaron durante una etapa... Todos vosotros habéis ayudado a dar forma a la persona que soy hoy. No hay un momento del que me arrepienta, un beso que desee nunca haber dado, una palabra que preferiría no haber oído. Ha sido un largo camino, pero esto es solo el comienzo. Las olas siguen llegando a la playa, las células siguen creciendo en nuestras placas de cultivo, y la vida, como el espectáculo, debe continuar. Lo único importante es ser feliz. Siempre hacia delante y apuntando alto, retroceder nunca, rendirse jamás (Corey Yuen, 1986).First of all, I specially want to thank Jose María, who has guided me through this life-changing process since the very beginning. He encouraged me to grow, and when it was not possible, he brought me a stool so I could always reach higher.With your help I became a researcher, and everything I learnt will be with me forever. Even though sometimes it was challenging to come to an agreement, this represents the core essence of research and science, and I truly appreciate it.Challenges always inspire us, make us learn, keep us active and take away our fear of creative solutions. And it is always better to have plenty of these.I also want to express my sincere thanks to Kim Boutilier. She opened her door to me on the first day, and still holds it open. Not only have I learnt new things from...

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Androgenetic haploids and SSR markers as tools for the development of tropical maize hybrids

Cited by 53 publications

References 23 publications

In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE

In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE

Verification and characterization of chromosome duplication in haploid maize

Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant

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