Androgen‐responsive and ‐unresponsive prostate cancer cell lines respond differently to stimuli inducing neuroendocrine differentiation

Marchiani, Sara; Tamburrino, Lara; Nesi, Gabriella; Paglierani, Milena; Gelmini, Stefania; Orlando, Claudio; Maggi, Mario; Forti, Gianni; Baldi, Elisabetta

doi:10.1111/j.1365-2605.2009.01030.x

Cited by 51 publications

(51 citation statements)

References 43 publications

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“…Caki‐1 and ACHN are metastatic RCC and Caki‐1 has better drug sensitivity to niclosamide and B17. DU145 and PC‐3 are androgen resistant PCa18 and DU145 has better drug sensitivity to niclosamide and B17. Hence, we choose T24, Caki‐1, and DU145 as the representative cell lines for further study.…”

Section: Resultsmentioning

confidence: 99%

A new niclosamide derivatives‐B17 can inhibit urological cancers growth through apoptosis‐related pathway

Chen

Huang

et al. 2018

Cancer Medicine

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The incidence and mortality rate of urological cancers is increasing yearly. Niclosamide has been repurposed as an anti‐cancer drug in recent years. Synthesized derivative of niclosamide was testified for its anti‐cancer activity in urological cancers. MTT assay was used to measure the cytotoxicity effect of niclosamide and its derivatives in urological cancer cell lines. Migratory ability was monitored by scratch migration assay. Apoptosis and cell cycle changes were analyzed by annexin V and PI staining. The apoptosis‐related signal proteins were evaluated by western blotting. T24 had the best drug sensitivity with the lowest IC 50 in niclosamide and B17 treatment than DU145 and Caki‐1 cells. After niclosamide and B17 treatment, the mitotic cells were decreased, but apoptotic bodies and morphology changes were not prominent in T24, Caki‐1, and DU145 cells. The migratory ability was inhibited in niclosamide treatment than control group on Caki‐1 cells and niclosamide and B17 treatment than control group on DU145 cells. Early apoptosis cells were increased after niclosamide and B17 treatment than control group without cell cycle changes in T24, Caki‐1, and DU145 cells. Programmed cell death was activated majorly through PAPR and bcl‐2 in T24 and caspase‐3 in Caki‐1 cells, respectively. Niclosamide and B17 derivative had good ability in inhibition proliferation and migratory ability in T24, Caki‐1, and DU145 cells without prominent morphology and apoptotic body changes. UCC cells are more sensitive to niclosamide and B17 treatment. Early apoptosis was induced after niclosamide and B17 treatment through different mechanisms in T24, Caki‐1, and DU145 cells.

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Section: Resultsmentioning

confidence: 99%

A new niclosamide derivatives‐B17 can inhibit urological cancers growth through apoptosis‐related pathway

Chen

Huang

et al. 2018

Cancer Medicine

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“…We decided to assess several different markers of NED at both the protein and mRNA level because the expression of different NED markers may not correlate in every experimental set-up, as was shown for γ-enolase and chromogranin A in LNCaP cells undergoing NED [24]. …”

Section: Resultsmentioning

confidence: 99%

The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells

et al. 2014

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BackgroundTumor heterogeneity and the plasticity of cancer cells present challenges for effective clinical diagnosis and therapy. Such challenges are epitomized by neuroendocrine transdifferentiation (NED) and the emergence of neuroendocrine-like cancer cells in prostate tumors. This phenomenon frequently arises from androgen-depleted prostate adenocarcinoma and is associated with the development of castration-resistant prostate cancer and poor prognosis.ResultsIn this study, we showed that NED was evoked in both androgen receptor (AR)-positive and AR-negative prostate epithelial cell lines by growing the cells to a high density. Androgen depletion and high-density cultivation were both associated with cell cycle arrest and deregulated expression of several cell cycle regulators, such as p27Kip1, members of the cyclin D protein family, and Cdk2. Dual inhibition of Cdk1 and Cdk2 using pharmacological inhibitor or RNAi led to modulation of the cell cycle and promotion of NED. We further demonstrated that the cyclic adenosine 3′, 5′-monophosphate (cAMP)-mediated pathway is activated in the high-density conditions. Importantly, inhibition of cAMP signaling using a specific inhibitor of adenylate cyclase, MDL-12330A, abolished the promotion of NED by high cell density.ConclusionsTaken together, our results imply a new relationship between cell cycle attenuation and promotion of NED and suggest high cell density as a trigger for cAMP signaling that can mediate reversible NED in prostate cancer cells.

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“…It has been debated whether the 2 prostate cancer cell lines PC-3 and DU-145 are androgen receptor positive or negative and whether or not they respond to hormone depletion (44). We therefore surveyed the literature for evidence of miRNA-guided differentiation between androgen-dependent or -independent prostate cancer.…”

Section: Prostate Cancermentioning

confidence: 99%

Global microRNA Analysis of the NCI-60 Cancer Cell Panel

Søkilde

Kaczkowski

Podolska

et al. 2011

Molecular Cancer Therapeutics

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MicroRNAs (miRNA) are a group of short noncoding RNAs that regulate gene expression at the posttranscriptional level. They are involved in many biological processes, including development, differentiation, apoptosis, and carcinogenesis. Because miRNAs may play a role in the initiation and progression of cancer, they comprise a novel class of promising diagnostic and prognostic molecular markers and potential drug targets. By applying an LNA-enhanced microarray platform, we studied the expression profiles of 955 miRNAs in the NCI-60 cancer cell lines and identified tissue-and cell-type-specific miRNA patterns by unsupervised hierarchical clustering and statistical analysis. A comparison of our data to three previously published miRNA expression studies on the NCI-60 panel showed a remarkably high correlation between the different technical platforms. In addition, the current work contributes expression data for 369 miRNAs that have not previously been profiled. Finally, by matching drug sensitivity data for the NCI-60 cells to their miRNA expression profiles, we found numerous drug-miRNAs pairs, for which the miRNA expression and drug sensitivity profiles were highly correlated and thus represent potential candidates for further investigation of drug resistance and sensitivity mechanisms. Mol Cancer Ther; 10(3); 375-84. Ó2011 AACR.

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Androgen‐responsive and ‐unresponsive prostate cancer cell lines respond differently to stimuli inducing neuroendocrine differentiation

Cited by 51 publications

References 43 publications

A new niclosamide derivatives‐B17 can inhibit urological cancers growth through apoptosis‐related pathway

A new niclosamide derivatives‐B17 can inhibit urological cancers growth through apoptosis‐related pathway

The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells

Global microRNA Analysis of the NCI-60 Cancer Cell Panel

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