Androgen receptors in rat testis

Evidence for an androgen receptor in porcine Leydig cells

“…Using various biochemical techniques, androgen recep¬ tors have been found in whole testis (Hansson et al 1974;Mulder et al 1975), seminiferous tubules Trivial names and systematic names of steroids and abbreviations are: methyltrienolone : 17ß-hydroxy-17ct-methyl-4,9,11-oestratrien-3-one;…”

“…Using various biochemical techniques, androgen recep¬ tors have been found in whole testis (Hansson et al 1974;Mulder et al 1975), seminiferous tubules Trivial names and systematic names of steroids and abbreviations are: -hydroxy-17ct-methyl-4,9,11-oestratrien-3-one; ORG 2058: 16a-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione; DES: diethylstilboestrol, 3,4-bis(p-hydroxyphenyl)-3-hexene; dexamethasone : 9a-fluoro-1 Iß, 17a,21-trihydroxy16a-methyl-1.4-pregnadiene-3,20-dione; DCC : dextran coated charcoal ;…”

Androgen Binding in the Testis: In Vitro Production of Androgen Binding Protein (ABP) by Sertoli Cell Cultures and Measurement of Nuclear Bound Androgen by a Nuclear Exchange Assay

“…Hansson et al (8) reported that the intracellular androgen receptors were of tubular origin and persisted for up to 24 days following hypophysectomy in immature rats. Mulder et al (19) found testicular nuclear uptake into 0.4M KCl extractable-m8terial 3-20 days after hypophysectomy in adult animals.…”

“…Carbon extraction has been reported to destroy the receptors labeled by in vivo injection (8). Reduction of local testosterone levels by hypophysectomy could conceivably cause degeneration of receptors themselves or of the cell types which possess them.…”

Biochemical aspects of the interaction of androgens with Sertoli cells

“…That the Sertoli cell can also respond to androgen (and hence indirectly to LH) was suggested by the fact that under certain circumstances production of ABP can also be regulated by androgens Means et al, 1976 ;Louis and Fritz, 1977). Furthermore, testicular androgen receptor-like macromolecules which persisted following hypophysectomy and germ cell depletion have been described (Hansson et al, 1974 ;Mulder et at., 1974 . The interaction of progestogens with androgen receptors in other target tissues has been described at some length by Bardin et al (1975).…”