Androgen insufficiency in women: diagnostic and therapeutic implications

Rivera-Woll, Livia; Papalia, Mary-Anne; Davis, Susan Ruth; Burger, Henry G.

doi:10.1093/humupd/dmh037

Cited by 61 publications

(43 citation statements)

References 93 publications

(110 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ideally, correlation analyses should be performed with clear clinical endpoints, such as degree of hirsutism or presence of the female androgen deficiency syndrome. However, until now, clear clinical endpoints related to androgen concentrations cannot irrefutably be identified (40)(41)(42). Therefore, the associations of androgens, measured by both assays, were compared with other physiologically related steroids (androgens and E 2 ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: The measurement of serum testosterone in women is challenging due to lack of trueness, precision, and sensitivity of various available testosterone assays. Accurate assessment of testosterone in women is crucial especially in conditions associated with alleged over-or under-production of testosterone, such as in polycystic ovary syndrome (PCOS) or primary ovarian insufficiency (POI). The aim of this study was to measure and compare androgen concentrations in women with PCOS, POI, and female controls and to evaluate the performance of extraction RIA and liquid chromatographytandem mass spectrometry (LC-MS/MS) in these women. Design: Cross-sectional study. Methods: Carefully phenotyped women with POI (nZ208) or PCOS (nZ200) and 45 healthy, regularly cyclic female controls were included. Method comparison analyses were performed for total testosterone, androstenedione (AD), and DHEA, as measured by LC-MS/MS and extraction RIA. Results: All androgen levels were significantly elevated in women with PCOS compared with POI patients (P!0.05) and controls (P!0.05). Women with POI presented with similar androgen concentrations as controls, except for AD. Compared with measurements by extraction RIA, testosterone, DHEA, and AD concentrations measured by LC-MS/MS were systematically lower. However, using extraction RIA and LC-MS/MS, testosterone, DHEA, and AD measurements were shown to have good agreement as assessed by Bland-Altman analysis and intraclass correlation coefficient: 0.95 (95% confidence interval 0. 94-0.91), 0.83 (0.79-0.86), and 0.96 (0.95-0.97) respectively. Conclusions: LC-MS/MS, compared with a labor-intensive extraction RIA, shows good precision, sensitivity, and high accuracy for measuring female testosterone, DHEA, and AD concentrations under various clinical conditions. LC-MS/MS, therefore, represents a convenient and reliable assay for both clinical and research purposes, where androgen measurement in women is required. European Journal of Endocrinology 165 925-933

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Clinical symptoms of the proposed deficiency state include decreased libido, sexual receptivity and pleasure ; a diminished sense of well-being ; dysphoric mood and/or blunted motivation ; and persistent unexplained fatigue. Clinical signs include bone loss, decreased muscle mass and strength, adipose tissue redistribution, decreased sexual hair and changes in cognition or memory (14). There are many causes of low testosterone level in women including dysfunction of the hypothalamic pituitary axis, surgical or medical oophorectomy, surgical or medical adrenalectomy, premature ovarian failure, Cushing syndrome, radiation and /or chemotherapy and thyroid disease.…”

Section: Female Androgen Insufficiencymentioning

confidence: 99%

Androgen in postmenopausal women

et al. 2012

View full text Add to dashboard Cite

Dynamic changes in circulating estradiol level, including increase at menarche and decrease at menopause, occur in a woman's lifetime. Circulating estradiol levels decrease drastically during the menopausal transition, though the levels differ among races. It has been reported that estradiol levels in both Japanese and Chinese women were lower than those in Caucasians, Hispanic and AfricanAmericans (1).Based on data obtained in a previous study (2), we re-analyzed data for 169 healthy men and 252 healthy women aged from 40 to 85 years in the population-based study with addition of data for premenopausal women. Change in circulating estradiol in women from the menopausal transition to postmenopause is characterized as follows : high estradiol level in premenopausal women is drastically decreased and estradiol level in postmenopausal women is significantly lower than that in agematched men. Mean estradiol levels were shown to be 20.1 pg/ml in men and 6.1 pg/ml in postmenopausal women (Figure 1). This dynamic decrease in REVIEW Androgen in postmenopausal women

show abstract

“…As puberty progresses, obvious gender differences appear mainly due to a rise in androgen and estrogen levels in men and women respectively. In women, androgen concentrations in plasma are many times higher than the concentration of estrogen, even during reproductive life (Rivera-Woll et al 2004). Some of these steroids may be considered as prohormones, being converted peripherally to estrogen and other biologically active androgens.…”

Section: Introductionmentioning

confidence: 99%

Gender difference of androgen actions on skeletal muscle transcriptome

Yoshioka¹,

Boivin²,

Bolduc³

et al. 2007

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Sarcopenia is related to metabolic syndrome in postmenopausal women. Hormone replacement therapies with androgens improve muscle functions by molecular mechanisms that are still unknown, at least partly because the skeletal muscle transcriptome has been less characterized in females. We performed the serial analysis of gene expression method in six experimental groups, intact (male and female), ovariectomy (OVX), OVXCdihydrotestosterone (DHT) injection 1, 3, or 24 h before kill in mice. The 438 transcript species differentially expressed between gender showed that females had higher expression levels of mRNA related to cytoskeleton/contractile apparatus and mitochondrial processes as well as protein, lipid, and amino acid metabolisms. In females, OVX and DHT modulated 109 and 128 transcript species respectively. OVX repressed transcripts of fast/glycolytic fiber, glycolysis, and glucose transport, whereas all these effects were reversed 3 h after the DHT injection. Moreover, DHT treatment induced transcripts which reduce intracellular Ca 2C level at early time points. These results may suggest that DHT treatment in OVX mice increases muscle contractility by affecting fiber distribution and intracellular Ca 2C concentration as well as improving glucose metabolism. On the other hand, transcripts of fast/oxidative fiber, oxidative phosphorylation, and ATP production were repressed 24 h after DHT administration. In our previous study using male mice, transcripts in oxidative phosphorylation and ATP production were induced 24 h after DHT injection (Yoshioka M, Boivin A, Ye P, Labrie F & St-Amand J 2006 Effects of dihydrotestosterone on skeletal muscle transcriptome in mice measured by serial analysis of gene expression. Journal of Molecular Endocrinology 36 247-259). These results demonstrate gender differences in DHT actions on skeletal muscle, and contribute to a precise understanding of the molecular mechanisms of androgen actions in the female skeletal muscle.

show abstract

Androgen insufficiency in women: diagnostic and therapeutic implications

Cited by 61 publications

References 93 publications

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Androgen in postmenopausal women

Gender difference of androgen actions on skeletal muscle transcriptome

Contact Info

Product

Resources

About