Androgen disruption of early development in Qurt strain medaka (Oryzias latipes)

León, Abimael; Teh, Swee J.; Hall, Linda C.; Teh, Foo Ching

doi:10.1016/j.aquatox.2007.02.012

Cited by 34 publications

(30 citation statements)

References 42 publications

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“…Qurt medaka ( Oryzias latipes ) cultured in the Aquatic Health Program at the University of California at Davis were used for the exposure study (Leon, 2007). Medaka eggs were collected from the culture system and maintained in embryo rearing solution (1 g/L NaCl, 0.030 g/L KCl, 0.040 g/L CaCl 2 .H 2 O, 80 mg/L MgSO 4 and 1 mg/L Methylene Blue in distilled water) until hatching.…”

Section: Methodsmentioning

confidence: 99%

Bioconcentration, metabolism and excretion of triclocarban in larval Qurt medaka (Oryzias latipes)

Schebb¹,

Flores

Kurobe

et al. 2011

Aquatic Toxicology

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The antimicrobial triclocarban (TCC) is frequently found in personal care products and commonly observed in surface waters and sediments. Due to its long environmental persistence TCC accumulates in sewage sludge. It also shows a high unintended biological activity as a potent inhibitor of the soluble epoxide hydrolase (sEH) and may be an endocrine disruptor. In this study, we investigated bioconcentration, metabolism and elimination of TCC in fish using Medaka (Oryzias latipes) as a model. Medaka larvae (7±1 days post hatching) were exposed to 63 nM (20 µg/L) TCC water for 24 hours. The LC-MS/MS analysis of water and tissues provided bioconcentration of TCC and its metabolites in fish body and rapid excretion into culture water. Results from tissue samples showed a tissue concentration of 34 µmol/kg and a log bioconcentration factor (BCF) of 2.86. These results are slightly lower than previous findings in snails and algae. A significant portion of the absorbed TCC was oxidatively metabolized by the fish to hydroxylated products. These metabolites underwent extensive phase II metabolism to yield sulfate and glucuronic acid conjugates. The most abundant metabolite in fish tissue was the glucuronide of 2’-OH-TCC. Elimination of TCC after transferring the fish to fresh water was rapid, with a half-life of 1 hour. This study shows that larval medaka metabolize TCC similarly to mammals. The rapid rate of metabolism results in a lower bioconcentration than calculated from the n-octanol/water partition coefficient of TCC.

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Section: Methodsmentioning

confidence: 99%

Bioconcentration, metabolism and excretion of triclocarban in larval Qurt medaka (Oryzias latipes)

Schebb¹,

Flores

Kurobe

et al. 2011

Aquatic Toxicology

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“…The appearance of histological sections, coupled with analysis of Vtg expression, is considered one of the most sensitive endpoints for determining whether endocrine disruption has occurred [67]. Exposure to both estrogenic and androgenic compounds has been shown to reduce GSI and increase the incidence of germ cell necrosis, which may represent an interruption of spermatogenesis [40], [68], [69]. Notably, moderate levels of necrosis were higher (marginally significant) and severe cases of necrosis were significantly higher and GSI in males was significantly lower at the urban beach, at which we detected the presence of both estrogenic and androgenic compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue sections were examined under a BH-2 Olympus microscope for common and/or significant lesions. Lesions in testes were qualitatively scored on a scale of 0 = not present, 1 = mild, 2 = moderate, and 3 = severe [40]. Although ovaries were also sectioned, most were not of a high enough quality to be scored.…”

Section: Methodsmentioning

confidence: 99%

From ‘Omics to Otoliths: Responses of an Estuarine Fish to Endocrine Disrupting Compounds across Biological Scales

Brander

Connon

et al. 2013

PLoS ONE

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Endocrine disrupting chemicals (EDCs) cause physiological abnormalities and population decline in fishes. However, few studies have linked environmental EDC exposures with responses at multiple tiers of the biological hierarchy, including population-level effects. To this end, we undertook a four-tiered investigation in the impacted San Francisco Bay estuary with the Mississippi silverside (Menidia audens), a small pelagic fish. This approach demonstrated links between different EDC sources and fish responses at different levels of biological organization. First we determined that water from a study site primarily impacted by ranch run-off had only estrogenic activity in vitro, while water sampled from a site receiving a combination of urban, limited ranch run-off, and treated wastewater effluent had both estrogenic and androgenic activity. Secondly, at the molecular level we found that fish had higher mRNA levels for estrogen-responsive genes at the site where only estrogenic activity was detected but relatively lower expression levels where both estrogenic and androgenic EDCs were detected. Thirdly, at the organism level, males at the site exposed to both estrogens and androgens had significantly lower mean gonadal somatic indices, significantly higher incidence of severe testicular necrosis and altered somatic growth relative to the site where only estrogens were detected. Finally, at the population level, the sex ratio was significantly skewed towards males at the site with measured androgenic and estrogenic activity. Our results suggest that mixtures of androgenic and estrogenic EDCs have antagonistic and potentially additive effects depending on the biological scale being assessed, and that mixtures containing androgens and estrogens may produce unexpected effects. In summary, evaluating EDC response at multiple tiers is necessary to determine the source of disruption (lowest scale, i.e. cell line) and what the ecological impact will be (largest scale, i.e. sex ratio).

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“…Females in both mammalian and fish species exposed to 17b-trenbolone during early life stages demonstrate masculinization, characterized by increased anogenital distance, decrease in nipple number, and increases in genital malformations among rats (Hotchkiss et al, 2007) and increases in ovotestis and male secondary sex characteristics in mosquitofish (Sone et al, 2005). Male medaka exposed during development to 11-ketotestosterone exhibited increases in thyroid follicular hypertrophy (León et al, 2007). Adult female medaka and fathead minnow exposed to 17b-trenbolone display increased development of male secondary sex characteristics (Seki et al, 2006) and decreased fecundity (Ankley et al, 2003).…”

Section: Introductionmentioning

confidence: 96%

Airborne particulate matter collected near beef cattle feedyards induces androgenic and estrogenic activity in vitro

Wooten

Blackwell

McEachran

et al. 2015

Agriculture, Ecosystems & Environment

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Androgen disruption of early development in Qurt strain medaka (Oryzias latipes)

Cited by 34 publications

References 42 publications

Bioconcentration, metabolism and excretion of triclocarban in larval Qurt medaka (Oryzias latipes)

Bioconcentration, metabolism and excretion of triclocarban in larval Qurt medaka (Oryzias latipes)

From ‘Omics to Otoliths: Responses of an Estuarine Fish to Endocrine Disrupting Compounds across Biological Scales

Airborne particulate matter collected near beef cattle feedyards induces androgenic and estrogenic activity in vitro

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