Ancient Evolutionary Origin of the Neural Crest Gene Regulatory Network

Sauka‐Spengler, Tatjana; Meulemans, Daniel; Jones, Matthew C.; Bronner‐Fraser, Marianne

doi:10.1016/j.devcel.2007.08.005

Cited by 225 publications

(260 citation statements)

References 70 publications

(94 reference statements)

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“…Adult lampreys (Petromyzon marinus) at different stages of sexual maturation were obtained from Hammond Bay Biological Station, Millersburg, MI. Eggs were fertilized, cultured and collected as described (7,35).…”

Section: Methodsmentioning

confidence: 99%

“…FITC-labeled MOs against Pax3/7, ZicA, MsxA, n-Myc, Id, and AP-2 were obtained from Gene Tools and used as described (7). The following amounts of MO were used for injection: 10 ng per cell of Pax3/7 MO, 20 ng/cell of n-Myc, MsxA, and Id MO, and 40 ng/cell of ZicA and AP-2 MO.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, a more comprehensive functional analysis of multiple components with the network was systematically tested in a single species (7). The results suggested that much of the NC-GRN is highly conserved across vertebrates (7), contrasting with nonvertebrate chordates (6,(8)(9)(10)(11)(12)(13)(14)(15), which appear to lack a module involved in neural crest specification (16).…”

mentioning

confidence: 99%

“…The basal-most extant vertebrates, lamprey and hagfish, are both agnathans (jawless vertebrates) that have migrating neural crest cells and most of the neural crest derivatives (7,(17)(18)(19)(20). Only lampreys reliably produce embryos that are accessible to experimental manipulation in the laboratory.…”

mentioning

confidence: 99%

“…In a previous study, we identified lamprey homologues of numerous neural crest network genes and examined their effects on the appearance of neural crest cells (7). However, the interactions among neural plate border specifiers were not clear because of the relatively late stage of analysis.…”

mentioning

confidence: 99%

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Dissecting early regulatory relationships in the lamprey neural crest gene network

Nikitina

Sauka‐Spengler²,

Bronner‐Fraser³

2008

Proc. Natl. Acad. Sci. U.S.A.

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The neural crest, a multipotent embryonic cell type, originates at the border between neural and nonneural ectoderm. After neural tube closure, these cells undergo an epithelial-mesenchymal transition, migrate to precise, often distant locations, and differentiate into diverse derivatives. Analyses of expression and function of signaling and transcription factors in higher vertebrates has led to the proposal that a neural crest gene regulatory network (NC-GRN) orchestrates neural crest formation. Here, we interrogate the NC-GRN in the lamprey, taking advantage of its slow development and basal phylogenetic position to resolve early inductive events, 1 regulatory step at the time. To establish regulatory relationships at the neural plate border, we assess relative expression of 6 neural crest network genes and effects of individually perturbing each on the remaining 5. The results refine an upstream portion of the NC-GRN and reveal unexpected order and linkages therein; e.g., lamprey AP-2 appears to function early as a neural plate border rather than a neural crest specifier and in a pathway linked to MsxA but independent of ZicA. These findings provide an ancestral framework for performing comparative tests in higher vertebrates in which network linkages may be more difficult to resolve because of their rapid development.neural plate border ͉ transcription factor ͉ agnathan

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Dissecting early regulatory relationships in the lamprey neural crest gene network

Nikitina

Sauka‐Spengler²,

Bronner‐Fraser³

2008

Proc. Natl. Acad. Sci. U.S.A.

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It's a long way from amphioxus: descendants of the earliest chordate

Garcia‐Fernàndez

Benito‐Gutiérrez²

2009

BioEssays

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The origin of chordates and the consequent genesis of vertebrates were major events in natural history. The amphioxus (lancelet) is now recognised as the closest extant relative to the stem chordate and is the only living invertebrate that retains a vertebrate-like development and body plan through its lifespan, despite more than 500 million years of independent evolution from the stem vertebrate. The inspiring data coming from its recently sequenced genome confirms that amphioxus has a prototypical chordate genome with respect to gene content and structure, and even chromosomal organisation. Pushed by joint efforts of amphioxus researchers, amphioxus is now entering a new era, namely its maturation as a laboratory model, through the availability of a large amount of molecular data and the advent of experimental manipulation of the embryo. These two facts may well serve to illuminate the hidden secrets of the genetic changes that generated, among other vertebrates, ourselves.

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The odontode explosion: The origin of tooth‐like structures in vertebrates

et al. 2010

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We integrate recent data to shed new light on the thorny controversy of how teeth arose in evolution. Essentially we show (a) how teeth can form equally from any epithelium, be it endoderm, ectoderm or a combination of the two and (b) that the gene expression programs of oral vs. pharyngeal teeth are remarkably similar. Classic theories suggest that (i) skin denticles evolved first and odontode-inductive surface ectoderm merged inside the oral cavity to form teeth (the 'outside-in' hypothesis) or that (ii) patterned odontodes evolved first from endoderm deep inside the pharyngeal cavity (the 'inside-out' hypothesis). We propose a new perspective that views odontodes as structures sharing a deep molecular homology, united by sets of co-expressed genes defining a competent thickened epithelium and a collaborative neural crest derived ectomesenchyme. Simply put, odontodes develop 'inside and out,' wherever and whenever these co-expressed gene sets signal to one another. Our perspective complements the classic theories and highlights an agenda for specific experimental manipulations in model and non-model organisms.

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Ancient Evolutionary Origin of the Neural Crest Gene Regulatory Network

Cited by 225 publications

References 70 publications

Dissecting early regulatory relationships in the lamprey neural crest gene network

Dissecting early regulatory relationships in the lamprey neural crest gene network

It's a long way from amphioxus: descendants of the earliest chordate

The odontode explosion: The origin of tooth‐like structures in vertebrates

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