Ancient bacterial DNA (aDNA) in dental calculus from archaeological human remains

Preus, Hans R.; Marvik, Ole J.; Selvig, Knut A.; Bennike, Pia

doi:10.1016/j.jas.2011.03.020

Cited by 51 publications

(35 citation statements)

References 29 publications

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“…Blatt et al (2010) found not only phytoliths and mineralized bacteria, but also cotton embedded in the calculus matrix, which provided the first direct evidence for prehistoric cotton in Ohio (Late Woodland, A.D. 900e1000). Recently, Preus et al (2011) have shown it is possible to analyze ancient bacterial DNA from calculus.…”

Section: Introductionmentioning

confidence: 99%

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Scott

Poulson

2012

Journal of Archaeological Science

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Section: Introductionmentioning

confidence: 99%

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Scott

Poulson

2012

Journal of Archaeological Science

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“…In addition, this bacterium was recently detected by other groups using the gold-labelled antibody transmission electron microscopy [43], and in 2012 an aDNA segment from S. mutans DNA was amplified from an ancient dental calculus of an individual dating from approximately 500 years BP [44]. Ten sequences from the Bronze Age to the beginning of the twentieth century from ancient dental carious lesions are presented in this study, allowing our team to carry out a phylogenetic analysis of the ancient strains of this bacterium.…”

Section: Discussionmentioning

confidence: 87%

Molecular analysis of ancient caries

et al. 2014

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An 84 base pair sequence of the Streptococcus mutans virulence factor, known as dextranase, has been obtained from 10 individuals from the Bronze Age to the Modern Era in Europe and from before and after the colonization in America. Modern samples show four polymorphic sites that have not been found in the ancient samples studied so far. The nucleotide and haplotype diversity of this region have increased over time, which could be reflecting the footprint of a population expansion. While this segment has apparently evolved according to neutral evolution, we have been able to detect one site that is under positive selection pressure both in present and past populations. This study is a first step to study the evolution of this microorganism, analysed using direct evidence obtained from ancient remains.

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“…9 In 2011, the preservation of bacterial DNA within dental calculus was confirmed by gold-labeled antibody transmission electron microscopy. 10 Adler et al used 16S rRNA gene amplicons to demonstrate that dental calculus preserves an oral microbiome profile.…”

Section: Historymentioning

confidence: 99%

Oral Paleomicrobiology: Study of Ancient Oral Microbiome

Dagli

Baroudi³

et al. 2015

The Journal of Contemporary Dental Practice

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Background Paleomicrobiology is a special branch of micropaleontology concerned with the study of bacterial fossils. We have used the term ‘oral paleomicrobiology’, as in this review we have focused on the ancient oral microflora. Recently, dental calculus and dental pulp have been identified as rich sources of ancient microbial DNA. Study of this ancient genetic material opens a new door to the ancient world. This review gives an overview of history of ancient DNA research, various techniques of analyzing ancient DNA in dental calculus and dental pulp, and the implications of the oral paleomicrobiology. Materials and methods A comprehensive literature search was performed in the following databases—pubmed, medline and google scholar for studies published before 10 April, 2015. The following keywords were used—‘ancient DNA’, ‘ancient oral flora, ‘oral paleomicrobiology’ and ‘oral microbiome’, ‘16S rRNA sequencing’. To obtain additional data, a manual search was performed using the reference lists of selected articles. Result As a result of literature search, 27 articles were found in pubmed, 12 in google scholar and one in medline. Eight more articles were selected from the reference list of selected articles. Conclusion The combination of microbiology and paleontology has brought a revolution in the study of human evolution and microbial communities. The naturally well-preserved samples of microbial DNA from dental pulp and microbial colonies trapped in dental calculus are a potential source of microbial genetic material, which will prove invaluable in resolving mysteries of the past. This may be a beginning of a new era of oral paleomicrobiology, which will contribute in our studies about prevention of disease by establishing symbiosis between human beings and their microbiome. How to cite this article Dagli N, Dagli R, Baroudi K, Tarakji B. Oral Paleomicrobiology: Study of Ancient Oral Microbiome. J Contemp Dent Pract 2015;16(7):588-594.

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Ancient bacterial DNA (aDNA) in dental calculus from archaeological human remains

Cited by 51 publications

References 29 publications

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis

Molecular analysis of ancient caries

Oral Paleomicrobiology: Study of Ancient Oral Microbiome

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