Few biblical episodes have generated more theological interpretation across the centuries than that of Jesus praying in the Garden of Gethsemane, where he appears fearfully to resist the divine will in the moments before the passion sequence is initiated. Scholars of the early modern period, however, have tended not to notice how central the scene became in the wake of Protestant and Catholic reformation developments, renewed calls for spiritual self-examination and the resurgent phenomenon of martyrdom. This article addresses this lacuna by arguing that, in the case of England, Jesus in Gethsemane not only held acute resonances across different confessions, but resulted in interpretations that perpetuated a new kind of subjectivity, and one that influenced modernity and its notions of the divided self in a state of faith and doubt.