Anchoring TGF-β1 on biomaterial surface via affinitive interactions: Effects on spatial structures and bioactivity

Xiao, Meng; Xiao, Jiangwei; Wu, Gang; Ke, Yu; Fang, Liming; Deng, Chunlin; Liao, Hua

doi:10.1016/j.colsurfb.2018.02.059

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…The use of TGF-β1-affinity peptide (HSNGLPL) to increase the sequestration of TGF-β1 by a substrate has been reported by several groups [99,100]. Ju et al [30] modified the GelMA hydrogel with methacrylated-HSNGLPL and investigated its effect on chondrogenesis and cartilage regeneration.…”

Section: Tgf-βmentioning

confidence: 99%

Gelatin Methacrylate Hydrogel for Tissue Engineering Applications—A Review on Material Modifications

et al. 2022

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To recreate or substitute tissue in vivo is a complicated endeavor that requires biomaterials that can mimic the natural tissue environment. Gelatin methacrylate (GelMA) is created through covalent bonding of naturally derived polymer gelatin and methacrylic groups. Due to its biocompatibility, GelMA receives a lot of attention in the tissue engineering research field. Additionally, GelMA has versatile physical properties that allow a broad range of modifications to enhance the interaction between the material and the cells. In this review, we look at recent modifications of GelMA with naturally derived polymers, nanomaterials, and growth factors, focusing on recent developments for vascular tissue engineering and wound healing applications. Compared to polymers and nanoparticles, the modifications that embed growth factors show better mechanical properties and better cell migration, stimulating vascular development and a structure comparable to the natural-extracellular matrix.

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Section: Tgf-βmentioning

confidence: 99%

Gelatin Methacrylate Hydrogel for Tissue Engineering Applications—A Review on Material Modifications

et al. 2022

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“…Solvent content/desolvation 25,26 Layer-by-layer deposition 29 Competitive adsorption 30 Protein structure/orientation [31][32][33] Mechanical properties 35 Antigen binding mechanisms 36…”

Section: Protein Biopolymersmentioning

confidence: 99%

“…Xiao et al bound a polyurethane polymer to a QCM-D sensor and grafted an affinity peptide via copper-catalyzed azidealkyne cycloaddition (CuAAC) click reaction to control the spatial organization and bioactivity of TGF-β. 32 Upon introducing TGF-β to this peptide-grafted layer, and comparing the QCM-D response to non-peptide-grafted and unmodified sensors, the structural organization of the TGF-β layer could be qualitatively visualized (Figure 3), including the affinity of TGF-β toward the peptide layer and any structural changes that occur after binding. The bioactivity of the layer was validated by an in vivo study in which the biopolymer successfully promoted inflammation and tissue regeneration in a mouse model.…”

Section: Relating Protein Structure To Bioactivitymentioning

confidence: 99%

Recent applications of QCM-D for the design, synthesis, and characterization of bioactive materials

Mosley

Talarico

Byrne

2021

Journal of Bioactive and Compatible Polymers

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The clinical translation of bioactive technologies is lacking compared to the number of novel technologies reported in the literature. This is in part due to the difficulties in characterizing bioactive materials to understand and predict their biological response. To progress the field and increase clinical success, more robust analytical techniques must be utilized when investigating novel bioactive materials. The quartz crystal microbalance with dissipation (QCM-D), a label-free sensing instrument based on an acoustic resonator, is used to quantify mass change and viscoelastic parameters from soft materials at the nanoscale, in situ, with precise temporal resolution and operation in both liquid and gaseous environments. The versatility of QCM-D has enhanced the characterization of bioactive polymers and sensing arrays for advanced applications of novel biotechnologies. In this review, we highlight exciting, recent applications of QCM-D for the investigation of bioactive materials. Attention is given to the dynamic mechanical properties of bioactive materials, discerning protein structure on surfaces, probing cell adhesion and cytoskeletal changes, and biosensing applications. We conclude that QCM-D has untapped utility in the pre-clinical investigation of bioactive materials and further utilization can improve the clinical success of novel technologies.

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“…Xiao et al coupled the affinitive peptide HSNGLPL to a polyurethane (PU) substrate via copper‐catalyzed azide–alkyne cycloaddition (CuAAC) click reaction, and implanted fragments of the material into mice gastrocnemius muscles. [ 146 ] Peptide‐grafted fragments with previously adsorbed TGF‐β1 greatly sped up the ensuing immune response and tissue regeneration. Increased collagen production was observed throughout the experimental period, and newly formed muscle fibers replaced the necrotic area surrounding the implants by day 21.…”

Section: Solid‐phase Presentationmentioning

confidence: 99%

“…These results provide a possible physical justification for the superior GF bioactivity when bound to its affinitive peptide. [ 146 ] However, in this study, exogenous GF was added to the scaffolds, and the authors do not make any comparison between the administered dose and the ones typically used with soluble administration. The same group has further shown that implantation of peptide‐grafted scaffold fragments without preadsorption of the GF also resulted in enrichment of TGF‐β1 at the implant site (likely produced by infiltrating macrophages).…”

Section: Solid‐phase Presentationmentioning

confidence: 99%

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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Anchoring TGF-β1 on biomaterial surface via affinitive interactions: Effects on spatial structures and bioactivity

Cited by 10 publications

References 24 publications

Gelatin Methacrylate Hydrogel for Tissue Engineering Applications—A Review on Material Modifications

Gelatin Methacrylate Hydrogel for Tissue Engineering Applications—A Review on Material Modifications

Recent applications of QCM-D for the design, synthesis, and characterization of bioactive materials

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

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