Anatomy and Physiology for General Nursing

Raju, SM; Madala, Bindu

doi:10.5005/jp/books/10049

Cited by 3 publications

(3 citation statements)

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“…In this regard, one of the main cellular functions that regulates viral infection and is closely related to the organization of MTs is the autophagy process [89][90][91][92][93][94]. Although the complexity of the interplay between autophagy and different ZIKV proteins in the context of virus infection and transmission has been reported [142,143,185,186], little is known about the ZIKV NS5 protein. Our observations indicate that the ZIKV NS5 protein negatively alters p62-autophagy flux in a dose-dependent manner, as indicated by the accumulation of p62 in cells overexpressing NS5.…”

Section: Discussionmentioning

confidence: 99%

The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor

Pérez-Yanes,

Lorenzo-Sánchez,

Cabrera-Rodríguez

et al. 2024

Cells

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Zika virus (ZIKV) infection and pathogenesis are linked to the disruption of neurogenesis, congenital Zika syndrome and microcephaly by affecting neural progenitor cells. Nonstructural protein 5 (NS5) is the largest product encoded by ZIKV-RNA and is important for replication and immune evasion. Here, we studied the potential effects of NS5 on microtubules (MTs) and autophagy flux, together with the interplay of NS5 with histone deacetylase 6 (HDAC6). Fluorescence microscopy, biochemical cell-fractionation combined with the use of HDAC6 mutants, chemical inhibitors and RNA interference indicated that NS5 accumulates in nuclear structures and strongly promotes the acetylation of MTs that aberrantly reorganize in nested structures. Similarly, NS5 accumulates the p62 protein, an autophagic-flux marker. Therefore, NS5 alters events that are under the control of the autophagic tubulin-deacetylase HDAC6. HDAC6 appears to degrade NS5 by autophagy in a deacetylase- and BUZ domain-dependent manner and to control the cytoplasmic expression of NS5. Moreover, NS5 inhibits RNA-mediated RIG-I interferon (IFN) production, resulting in greater activity when autophagy is inhibited (i.e., effect correlated with NS5 stability). Therefore, it is conceivable that NS5 contributes to cell toxicity and pathogenesis, evading the IFN-immune response by overcoming HDAC6 functions. HDAC6 has emerged as an anti-ZIKV factor by targeting NS5.

show abstract

Section: Discussionmentioning

confidence: 99%

The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor

Pérez-Yanes,

Lorenzo-Sánchez,

Cabrera-Rodríguez

et al. 2024

Cells

View full text Add to dashboard Cite

show abstract

“…In this regard, one of the main cellular functions that regulates viral infection and is closely related to the organization of MTs is the autophagy process [89][90][91][92][93][94]. Although the complexity of the interplay between autophagy and different ZIKV proteins in the context of virus infection and transmission has been reported [142,143,177,178], little is known about the ZIKV NS5 protein. Our observations indicate that the ZIKV NS5 protein negatively alters p62-autophagy flux in a dosedependent manner, as indicated by the accumulation of p62 in cells overexpressing NS5.…”

Section: Discussionmentioning

confidence: 99%

The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor

Pérez-Yanes,

Lorenzo-Sánchez,

Cabrera-Rodríguez

et al. 2024

Preprint

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Zika virus (ZIKV) infection and pathogenesis are linked to the disruption of neurogenesis, congenital Zika syndrome and microcephaly by affecting neural progenitor cells. Nonstructural protein 5 (NS5) is the largest product encoded by ZIKV-RNA and is important for replication and immune evasion. Here, we studied the potential effects of NS5 on microtubules (MTs) and autophagy flux, together with the interplay of NS5 with histone deacetylase 6 (HDAC6). Fluorescence microscopy, biochemical cell-fractionation combined with the use of HDAC6 mutants, chemical inhibitors and RNA interference indicated that NS5 accumulates in nuclear structures and strongly promotes the acetylation of MTs that aberrantly reorganize in nested-like structures. Similarly, NS5 accumulates the p62 protein, an autophagic-flux marker. Therefore, NS5 alters events that are under the control of the autophagic tubulin-deacetylase HDAC6. HDAC6 appears to degrade NS5 by autophagy in a deacetylase- and BUZ domain-dependent manner and to control the cytoplasmic expression of NS5. Moreover, NS5 inhibits RNA-mediated RIG-I interferon (IFN) production, resulting in greater activity when autophagy is inhibited (i.e., effect correlated with NS5 stability). Therefore, it is conceivable that NS5 contributes to cell toxicity and pathogenesis, evading the IFN-immune response by overcoming HDAC6 functions. HDAC6 has emerged as an anti-ZIKV factor by targeting NS5.

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“…Da Bindu reports many cases of assaults on women travelling to the FTZs in Sri Lanka, particularly since they often work late into the night. Organizing strategies have included efforts to ensure that workers always travel together and that they all shout out if they think any one of them is in danger (Da Bindu 1990). Other issues may relate to the difficulties of actually getting to work.…”

Section: Organizing In Asiamentioning

confidence: 99%

Women Working Worldwide: transnational networks, corporate social responsibility and action research

Hale

Wills

2007

Global Networks

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Anatomy and Physiology for General Nursing

Cited by 3 publications

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The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor

The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor

The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor

Women Working Worldwide: transnational networks, corporate social responsibility and action research

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