The genera Strobilanthes Blume have a rich history in therapeutic culture all over the world. Asian countries like India, China, Myanmar and Thailand still use Strobilanthes genus-based medicinal preparations for various diseases. Strobilanthes jomyi is a newly discovered species from Kerala, India. Some tribal communities of Kasaragod district still use S. jomyi leaf extract as a wound healing medication. The current study aims to investigate the pharmacognostic, phytochemical and GC-MS analysis of the leaves, stems and roots of S. jomyi. The microscopic, macroscopic, organoleptic, fluorescent, phytochemicals and GC-MS analysis of the leaves, stem, and root of S. jomyi were estimated using various standard protocols. The macroscopic and microscopic characters of leaves revealed the presence of non-glandular trichomes with paracytic stomata in the leaves. The transverse section of the stem and petiole showed the presence of raphides and the root showed the presence of tannin cells. Cystoliths were observed only in the petiole. Powder morphology of leaves, stems and roots revealed the presence of fibers, trichomes, palisade cells, spiral xylem vessels, bordered pit vessels and raphides. The vegetative part of S. jomyi powder exhibited various fluorescent coloration based on numerous chemical treatments along with different tastes, smells, colors and textures by organoleptic assays. Qualitative phytochemical analysis of different vegetative parts revealed the presence of flavonoids and other phytochemicals. GC-MS study revealed that lupeol a significant bioactive compound was present in all the vegetative parts of S. jomyi. The results acquired from this study can be used for the standardization, identification, quality and purity check of plant samples.
The genera Strobilanthes Blume have a rich history in therapeutic culture all over the world. Asian countries like India, China, Myanmar and Thailand still use Strobilanthes genus-based medicinal preparations for various diseases. Strobilanthes jomyi is a newly discovered species from Kerala, India. Some tribal communities of Kasaragod district still use S. jomyi leaf extract as a wound healing medication. The current study aims to investigate the pharmacognostic, phytochemical and GC-MS analysis of the leaves, stems and roots of S. jomyi. The microscopic, macroscopic, organoleptic, fluorescent, phytochemicals and GC-MS analysis of the leaves, stem, and root of S. jomyi were estimated using various standard protocols. The macroscopic and microscopic characters of leaves revealed the presence of non-glandular trichomes with paracytic stomata in the leaves. The transverse section of the stem and petiole showed the presence of raphides and the root showed the presence of tannin cells. Cystoliths were observed only in the petiole. Powder morphology of leaves, stems and roots revealed the presence of fibers, trichomes, palisade cells, spiral xylem vessels, bordered pit vessels and raphides. The vegetative part of S. jomyi powder exhibited various fluorescent coloration based on numerous chemical treatments along with different tastes, smells, colors and textures by organoleptic assays. Qualitative phytochemical analysis of different vegetative parts revealed the presence of flavonoids and other phytochemicals. GC-MS study revealed that lupeol a significant bioactive compound was present in all the vegetative parts of S. jomyi. The results acquired from this study can be used for the standardization, identification, quality and purity check of plant samples.
“…Fixed tissues were dehydrated by ethanol series (65, 75, 85, 95, 100% v/v), immersed in paraffin for 40 min, and embedded using a YB-6LF system (Yaguang Co.; Hubei, China). Thin sections (2.5 µm) were cut by rotary microtome (model HM 340E, Thermo Scientific; Hennigsdorf, Germany), stained with Safranin O/Fast Green [16], and observed with light microscope (model Axio Vert A1, Zeiss; Oberkochen, Germany) at ×400 magnification.…”
Section: Weight Loss and Browning Degreementioning
Flammulina velutipes was treated with 10, 50, and 250 μL L−1 isoamyl isothiocyanate (IAITC), and effects on quality preservation were assessed. IAITC displayed positive effects during the storage of F. velutipes: (i) maintained tissue integrity, reduced weight loss rates; (ii) reduced browning degree by inhibiting the activities of browning-related enzymes; (iii) enhanced activities of two antioxidant enzymes (superoxide dismutase and catalase) and increased ascorbic acid content; (iv) ameliorated microbial spoilage by inhibiting bacterial growth, especially Serratia, a major bacterial community on the surface of F. velutipes; (v) enhanced chitin content and thereby maintained structural integrity; and (vi) maintained levels of Asp, Glu, linoleic acid, and polyunsaturated fatty acids. In conclusion, IAITC reduced catalase activity and total phenolic content in F. velutipes during storage. The most suitable concentration of IAITC in the preservation of F. velutipes was 50 µL L−1.
“…The anatomical characters such as the absence or presence of trichomes, epidermal cell shape, and anticlinal cell wall patterns had taxonomic value and are useful in the identification of taxa. A detailed Anatomical study of Picris japonica Thunb., (Asteraceae), a herbal medicinal plant,was helpful for species-level identification,botanical quality control, and also to detect adulterations in commercial or in laboratory samples (Wang et al, 2018).…”
The comparative study of plant structure, morphology, and anatomy has always been the backbone of plant systematics. Anatomical characteristics are important evidence when elucidating the relationships among higher plants. Therefore the anatomical information can be taxonomically useful without having obvious evolutionary or phylogenetic interpretation. This review is a comprehensive study of the plant anatomical methods used by various researchers using different plant sources like leaf, petiole, and stem
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