Anatomopathological and Immunohistochemical Study of Explanted Cryopreserved Arteries

Rendal-Vázquez, María Esther; Verdes, Anahí San Luis; Otero, Jorge Pombo; Iglesias, R Segura; Doménech, Nieves; Núñez, C. Andión

doi:10.1016/j.avsg.2011.11.032

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…12,27,28 Immunological reaction may be responsible for graft destruction and thrombosis causing fragmentation of elastin, cellular accumulation, capillary ingrowth, endothelial thickening, and fibrotic stenosis as presented in morphological studies. 28,29 No occlusions among vein grafts were seen in the present study. When cryopreserved conduits were introduced at 2012 in HUH, both, arterial and venous allografts were used.…”

Section: Discussioncontrasting

confidence: 43%

Cryopreserved Venous Allografts in Supra-inguinal Reconstructions: A Single Centre Experience

Heinola

Kantonen

Mattila

et al. 2019

European Journal of Vascular and Endovascular Surgery

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WHAT THIS PAPER ADDS The current study introduces arterial reconstructions with cryopreserved femoral and vena caval vein allografts as a novel technique in the supra-inguinal setting with a low rate of re-infections and acceptable number of graft re-interventions on mid term analysis. Objective: This study introduces a novel technique for supra-inguinal arterial reconstructions with cryopreserved femoral vein and caval allografts with a low re-infection rate and an acceptable graft re-intervention rate on early mid term analysis. Methods: Patients treated from February 2012 to March 2018 with cryopreserved venous allograft reconstructions owing to infection in the supra-inguinal area were reviewed retrospectively. The primary end points were re-infection and the treatment related mortality rate. Secondary end points were 30 and 90 day and overall mortality and graft re-intervention rate. Results: Of the 23 patients treated with cryopreserved venous allografts for infection in aorto-iliac area, 21 (91%) patients underwent reconstruction with cryopreserved femoral veins and two (9%) with vena cava. Indications for treatment were aortic graft infections (n ¼ 12 [52%]), mycotic aneurysms (n ¼ 5 [22%]), femorofemoral prosthetic infections (n ¼ 3 [13%]), anastomotic pseudo-aneurysms (n ¼ 2 [9%]), and aortic thrombosis with intestinal spillage (n ¼ 1 [4%]). In hospital and 90 day mortality were 9% (n ¼ 2); overall treatment related mortality during the median follow up of 15 months was 13% (n ¼ 3). During the follow up, two allografts were re-operated on owing to anastomotic dilatation and one because of re-infection, resulting in a reintervention rate of 13% (n ¼ 3). None of the grafts was lost and there were no amputations. At the end of follow up 17 patients (74%) were alive. KaplaneMeier estimation for survival was 76% (95% confidence interval [CI] 57%e95%) at one year and 70% (95% CI 49%e91%) at two years. Conclusion: Cryopreserved venous allografts appear to be an infection resistant and reasonably safe reconstruction material in the aorto-iliac axis based upon the early mid term analysis from a single centre experience. Further research is needed to compare their performance with other biological reconstruction material.

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Section: Discussioncontrasting

confidence: 43%

Cryopreserved Venous Allografts in Supra-inguinal Reconstructions: A Single Centre Experience

Heinola

Kantonen

Mattila

et al. 2019

European Journal of Vascular and Endovascular Surgery

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“…Cryopreserved arterial grafts have been used since the 1990s in vascular surgery for replacement of infected prosthesis and in revascularization of peripheral vessels when venous grafts are not available and the patient is at high risk of graft infection if synthetic grafts are used . In contrast few reports about their use in outflow reconstruction in LDLT are available .…”

Section: Discussionmentioning

confidence: 99%

Cryopreserved arterial grafts as a conduit in outflow reconstruction in living donor liver transplantation

Ali

Yong

Eng

et al. 2015

J Hepato Biliary Pancreat

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“…The ability to replace tissues on demand would help to decrease the mortality rate in many clinical scenarios. Off-the-shelf available vascular grafts and heart valves for example would be needed for bypass surgery 1 , 2 , cardiac valvular pathologies including rheumatic fever, atherosclerosis in elderly patients or congenital malformation in children 3 – 6 , shunts for dialysis patients 7 , 8 and vessel reconstruction after organ transplantation 9 , 10 . Therefore, improved strategies and techniques for tissue preservation have to be developed to tackle this urgent and so far unmet medical need 11 .…”

Section: Introductionmentioning

confidence: 99%

The choice of cryopreservation method affects immune compatibility of human cardiovascular matrices

Schneider

Stamm

Brockbank

et al. 2017

Sci Rep

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Conventional frozen cryopreservation (CFC) is currently the gold standard for cardiovascular allograft preservation. However, inflammation and structural deterioration limit transplant durability. Ice-free cryopreservation (IFC) already demonstrated matrix structure preservation combined with attenuated immune responses. In this study, we aim to explore the mechanisms of this diminished immunogenicity in vitro. First, we characterized factors released by human aortic tissue after CFC and IFC. Secondly, we analyzed co-cultures with human peripheral blood mononuclear cells, purified monocytes, T cells and monocyte-derived macrophages to examine functional immune effects triggered by the tissue or released cues. IFC tissue exhibited significantly lower metabolic activity and release of pro-inflammatory cytokines than CFC tissue, but surprisingly, more active transforming growth factor β. Due to reduced cytokine release by IFC tissue, less monocyte and T cell migration was detected in a chemotaxis system. Moreover, only cues from CFC tissue but not from IFC tissue amplified αCD3 triggered T cell proliferation. In a specifically designed macrophage-tissue assay, we could show that macrophages did not upregulate M1 polarization markers (CD80 or HLA-DR) on either tissue type. In conclusion, IFC selectively modulates tissue characteristics and thereby attenuates immune cell attraction and activation. Therefore, IFC treatment creates improved opportunities for cardiovascular graft preservation.

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Anatomopathological and Immunohistochemical Study of Explanted Cryopreserved Arteries

Cited by 6 publications

References 30 publications

Cryopreserved Venous Allografts in Supra-inguinal Reconstructions: A Single Centre Experience

Cryopreserved Venous Allografts in Supra-inguinal Reconstructions: A Single Centre Experience

Cryopreserved arterial grafts as a conduit in outflow reconstruction in living donor liver transplantation

The choice of cryopreservation method affects immune compatibility of human cardiovascular matrices

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