Anatomically shaped osteochondral constructs for articular cartilage repair

Hung, Clark T.; Lima, Eric G.; Mauck, Robert L.; Taki, Erica; LeRoux, Michelle A.; Lu, Helen H.; Stark, Robert; Guo, Xing; Ateshian, Gerard A.

doi:10.1016/s0021-9290(03)00213-6

Cited by 194 publications

(166 citation statements)

References 68 publications

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“…Motivated by the fact that no biological based therapies exist to treat patients with osteoarthritis a number of studies have investigated engineering anatomically accurate osteochondral grafts for joint condyle repair [21][22][65][66]. The endochondral approach described in this study may be a powerful tool in scaling-up the osseous phase of such grafts as it is possible to engineer large cartilaginous grafts in vitro using MSCs as the low oxygen conditions that develop within these constructs supports chondrogenic differentiation and the functional development of the engineered tissue [57].…”

Section: Discussionmentioning

confidence: 99%

Engineering osteochondral constructs through spatial regulation of endochondral ossification

et al. 2013

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Chondrogenically primed bone marrow derived mesenchymal stem cells (MSCs) have been shown to become hypertrophic and undergo endochondral ossification when implanted in vivo. Modulating this endochondral phenotype may be an attractive approach to engineering the osseous phase of an osteochondral implant. The objective of this study was to engineer an osteochondral tissue by promoting endochondral ossification in one layer of a bi-layered construct and stable cartilage in the other. The top-half of bi-layered agarose hydrogels were seeded with culture expanded chondrocytes (termed chondral layer) and the bottom half of the bi-layered agarose hydrogels with MSCs (termed osseous layer). Constructs were cultured in a chondrogenic medium for 21 days and thereafter were either maintained in a chondrogenic medium, transferred to a hypertrophic medium, or implanted subcutaneously into nude mice. This structured chondrogenic bi-layered co-culture was found to enhance chondrogenesis in the chondral layer, appearing to help re-establish the chondrogenic phenotype that is lost in chondrocytes during monolayer expansion. Furthermore, the bilayered co-culture appeared to suppress hypertrophy and mineralisation in the osseous layer.The addition of hypertrophic factors to the media was found to induce mineralisation of the osseous layer in vitro. A similar result was observed in vivo where endochondral ossification was restricted to the osseous layer of the construct leading to the development of an osteochondral tissue. This novel approach represents a potential new treatment strategy for the repair of osteochondral defects.

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Section: Discussionmentioning

confidence: 99%

Engineering osteochondral constructs through spatial regulation of endochondral ossification

et al. 2013

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“…Natural materials, including collagen [16,17] fibrin [18], hyaluronan [19], alginate [20] and agarose [21][22][23], have been widely investigated for use in osteochondral tissue repair.…”

Section: Introductionmentioning

confidence: 99%

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

et al. 2014

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“…Bioreactor systems have also been developed to aid the cultivation of an anatomically shaped tissue-engineered patella [55]. Data for the patella geometry was obtained via stereophotogrammetry and used to drive a milling machine by means of computer-aided design (CAD) software.…”

Section: Bioreactors For Anatomically Shaped Graftsmentioning

confidence: 99%

Engineering custom-designed osteochondral tissue grafts

Grayson

Chao

Marolt

et al. 2008

Trends in Biotechnology

122

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Tissue engineering is expected to help us outlive the failure of our organs by enabling the creation of tissue substitutes capable of fully restoring the original tissue function. Degenerative joint disease, which affects one-fifth of the US population and is the country's leading cause of disability, drives current research of actively growing, functional tissue grafts for joint repair. Toward this goal, living cells are used in conjunction with bio-material scaffolds (serving as instructive templates for tissue development) and bioreactors (providing environmental control and molecular and physical regulatory signals). In this review, we discuss the requirements for engineering customized, anatomically-shaped, stratified grafts for joint repair and the challenges of designing these grafts to provide immediate functionality (load bearing, structural support) and long-term regeneration (maturation, integration, remodeling).

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Anatomically shaped osteochondral constructs for articular cartilage repair

Cited by 194 publications

References 68 publications

Engineering osteochondral constructs through spatial regulation of endochondral ossification

Engineering osteochondral constructs through spatial regulation of endochondral ossification

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

Engineering custom-designed osteochondral tissue grafts

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