Anatomical observations of adventitious bud regeneration from leaf explants of Ziziphus jujube Mill. ‘Huizao’

Ma, Chunhua; Xia, Ye; Chen, Yanhui; Feng, Jiancan; Shang, Xiaoli; Li, Jidong; Wu, Yingxia; Hu, Jianbin

doi:10.1007/s13580-012-0081-8

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…We observed that some of the dysplasia of floral organs in Z. jujuba 'Jinsi No. 4', suggesting that the dysplasia of floral organs may be one of the main reasons for low yields. In the process of bud differentiation of 'Jinsi No.4', we should supply sufficient nutrients to avoid malnutrition in the April.…”

Section: Resultsmentioning

confidence: 99%

“…jujuba 'Jinsi No. 4' was originated in the Yuncheng County, ShanXi Province, China. As a high-value, well known, and first grade product and rare species, the fruit of Z. jujuba has a sugar content of 22.75%, an acid content of 0.368%, soluble solids content of 26.5%, Vc (Vitamin C) content of 292.5 mg/100 g, and the edible percentage is as high as 97%.…”

Section: Introductionmentioning

confidence: 99%

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Flower Bud Differentiation and Development of ‘Jinsi No.4’ Jujube (Ziziphus jujuba Mill.) in Hunan Province of Southern China

Feng¹,

Duan²,

Xiong³

et al. 2017

TOBIOTJ

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Ziziphus jujuba Mill. is one of the most important fruit crops and has been cultivated in China for more than 4000 years. Z. jujuba fruit is rich in nutritional and medicinal values. Compared to other wood fruits, Z. jujuba is unique in its flowering and fruiting characteristics. Floral buds differentiation and formation of Z. jujuba is an essential process that affects yield. Z. jujuba 'Jinsi No.4' blooms profusely, yet its final yield is low. In this study, the floral bud differentiation and development of 'Jinsi No.4' were examined by paraffin section. Results showed that the floral buds of 'Jinsi No.4' differentiated in the current year and started from early April. The duration of a single flower differentiation was short, taking only 7 days for maturation of flowers buds. Floral bud differentiation of 'Jinsi No.4' can be divided into six stages, i.e., pre-differentiation, initial differentiation, sepal differentiation, petal differentiation, stamen differentiation, and pistil differentiation. Flower development experienced seven stages, i.e., alabastrum, alabastrum break, sepal flattening, petal flattening, stamen flattening, filament withering, and ovule swelling. Dysplasia was observed in some floral organs in Z. jujuba 'Jinsi No.4', suggesting that the dysplasia of floral organs may be one of the main reasons for low yields. Our findings on flower bud development in 'Jinsi No.4' will contribute to its production and flowering management in Hunan area of southern China.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Flower Bud Differentiation and Development of ‘Jinsi No.4’ Jujube (Ziziphus jujuba Mill.) in Hunan Province of Southern China

Feng¹,

Duan²,

Xiong³

et al. 2017

TOBIOTJ

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“…The tissue culture of Z. jujuba Mill. was successfully obtained for several distinguished cultivars via leaf (Li et al, 2002;Gu et al, 2005;Chen et al, 2008;Feng et al, 2010;Ma et al, 2012;Ye et al, 2012), embryos Qi, 2003, Kim et al, 2006), cotyledons (Kim et al, 2006) and nodal ex-plants (Goyal et al, 2006;Soliman and Hegazi, 2013). However, the response of the planting material on tissue culture media depends on the genotype and therefore, it is important to develop a proper in-vitro protocol for Jujube varieties adapted to Sri Lankan condition to promote the commercial scale cultivation.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

Micropropagation of <i>Ziziphus jujube</i> Mill. (Jujube) through shoot tip and nodal segment culture

Hansika¹,

Ranawake²,

Perera³

2017

Trop Agric Res & Ext

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Jujube is known as Masan in Sri Lanka is one of the underutilized fruit crops. It is important due to its high nutritious value and introduced as a potential crop for commercial cultivation. Micropropagation using plant tissue culture is an efficient method for vegetative propagation of commercially important crops for mass production to meet the demands of planting materials. Present study was carried out to develop a proper in-vitro protocol for local varieties of Jujube to produce planting materials. The most suitable fungicide for surface sterilization procedure was selected by testing Captan (Captan 50%, 1.2 g/l), Topsin (Thiophanate methyl 70%, 2 g/l), and Daconil (Chlorothalonil, 1.8 ml/l). Selection of a suitable concentration of BAP (Benzyle Amino Purine) or TDZ (Thidiazuron) for shoot proliferation was assessed in four different concentrations of BAP (1, 1.5, 2, 2.5 mg/l) and of TDZ (0.1, 0.2, 0.3, 0.4 mg/l) and IBA (Indole Butric Acid) for root induction in two concentrations of IBA (1, 2 mg/l). Dipping shoot tips in Captan solution for 20 minutes gave the highest significant non-contamination percentage (79.9%) and lowest fungal radius (0.15 cm) of contaminated cultures among three treatments. MS (Murrashige and Skoog) medium containing 1.5 mg/l BAP recorded significantly highest percentage of elongated bud (96.66%) and newly produced shoot length (1.08 cm) and lowest significant rate was recorded in TDZ 0.2 mg/l (3.33%). Callus was produced in all the concentrations of TDZ. None of the concentrations of BAP or TDZ produced multiple shoots. Elongated nodal segments in BAP (1, 1.5 mg/l) could be successfully sub-cultured for further multiplication. Rooting was not recorded in both shoot tips and in-vitro generated shoots during four weeks of culturing on IBA contained media.

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“…Leaf explants did not show any response on an MMS medium alone, or an MMS containing 0.5 or 1.0 μM TDZ, while a callus was induced from explants on a medium containing 20.0 μM TDZ (data not shown). The largest number of shoots was induced from leaf explants either on a medium containing TDZ alone or in combination with other growth regulators in Saintpaulia ionantha (Mithila et al, 2003), Arachis correntina (Mroginski et al, 2004), Hydrangea quercifolia (Ledbetter and Preece, 2004), Vaccinium vitis-idaea (Debnath, 2005), Pautownia tomentosa (Corredoira et al, 2008), Vaccinum corymbosum (Liu et al, 2010), Phyllanthus amarus (Nitnaware et al, 2011), Viburnum dentatum (Dai et al, 2011) and Ziziphus jujube (Ma et al, 2012). Plant regeneration in vitro was dependent on the concentration of plant growth regulators in the culture medium.…”

Section: Induction Of Adventitious Shoots From Leaf Explantsmentioning

confidence: 99%

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Kumar¹,

Ku²,

Agrawal³

et al. 2013

bta

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In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron (TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants. It appeared that the frequency of protuberance development depended on the concentration of TDZ. However, this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully established in a greenhouse.

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Anatomical observations of adventitious bud regeneration from leaf explants of Ziziphus jujube Mill. ‘Huizao’

Cited by 6 publications

References 13 publications

Flower Bud Differentiation and Development of ‘Jinsi No.4’ Jujube (Ziziphus jujuba Mill.) in Hunan Province of Southern China

Flower Bud Differentiation and Development of ‘Jinsi No.4’ Jujube (Ziziphus jujuba Mill.) in Hunan Province of Southern China

Micropropagation of <i>Ziziphus jujube</i> Mill. (Jujube) through shoot tip and nodal segment culture

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

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