Anatomical Investigation and GC/MS Analysis of ‘Coco de Mer’,<i>Lodoicea maldivica</i>(Arecaceae)

Giuliani, C.; Pieraccini, Giuseppe; Santilli, Carolina; Tani, Corrado; Bottoni, Martina; Schiff, Silvia; Fico, Gelsomina; Papini, Alessio; Falsini, Sara

doi:10.1002/cbdv.202000707

Cited by 10 publications

(5 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These sections were then stained. Fluorol Yellow 088 was used to discern the presence of lipid material (Bundrett et al, 1991 , as modified in Giuliani et al, 2020 ) within the lining cells of PARD-like structures to see whether lipid material had been secreted into the duct space/cavity. Sudan Red 7B (Lison, 1960 ) confirmed the presence of the fatty acid component of the resin.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructure of Terpene and Polyphenol Synthesis in the Bark of Cupressus sempervirens After Seiridium cardinale Infection

Rocca

Papini²,

Posarelli³

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Cypress Canker Disease (CCD) pandemic caused by Seiridium cardinale is the major constraint of many Cupressaceae worldwide. One of the main symptoms of the disease is the flow of resin from the cankered barks. While inducible phloem axial resin duct-like structures (PARDs) have recently been characterized from an anatomical point of view, their actual resin production is still being debated and has never been demonstrated. Although the involvement of polyphenolic parenchyma cells (PP cells) in the bark of Cupressus sempervirens after S. cardinale infection was revealed in one of our previous studies using light microscopy, their evolution from the phloem parenchyma cells is yet to be clarified. This study investigated functional and ultrastructural aspects of both PARD-like structures and PP cells by means of more in-depth light (LM) and fluorescence microscopy (FM) combined with histochemical staining (using Sudan red, Fluorol Yellow, NADI Aniline blue black, and Toluidine blue staining), in addition to Transmission Electron Microscope (TEM). Two-year-old stem sections of a C. sempervirens canker-resistant clone (var. “Bolgheri”), artificially inoculated with S. cardinale, were sampled 5, 7, 14, 21, and 45 days after inoculation, for time-course observations. FM observation using Fluorol yellow dye clearly showed the presence of lipid material in PARD-like structures lining cells of the cavity and during their secretion into the duct space/cavity. The same tissues were also positive for NADI staining, revealing the presence of terpenoids. The cytoplasm of the ducts' lining cells was also positive for Sudan red. TEM observation highlighted the involvement of plastids and endoplasmic reticulum in the production of terpenoids and the consequent secretion of terpenoids directly through the plasma membrane, without exhibiting vesicle formation. The presence of a high number of mitochondria around the area of terpenoid production suggests that this process is active and consumes ATP. The LM observations showed that PP cells originated from the phloem parenchyma cells (and possibly albuminous cells) through the accumulation of phenolic substances in the vacuole. Here, plastids were again involved in their production. Thus, the findings of this work suggest that the PARD-like structures can actually be considered PARDs or even bark traumatic resin ducts (BTRD).

show abstract

Section: Methodsmentioning

confidence: 99%

Ultrastructure of Terpene and Polyphenol Synthesis in the Bark of Cupressus sempervirens After Seiridium cardinale Infection

Rocca

Papini²,

Posarelli³

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Senyawa sesquiterpen mempunyai efek yang beragam sebagai antimikroba, antifungi dan antibiotik (Ali et al, 2015;Cui et al, 2011). Alloaromadendrene juga memiliki potensi sebagai antiaging dan antioksidan karena memiliki aktivitas mengurangi resistensi stress oksidatif yang diinduksi oleh juglone (Giuliani et al, 2020).…”

Section: Hasil Analisa Kandungan Senyawa Minyak Atsiri Gaharuunclassified

Pengaruh Pretreatment Microwave Terhadap Rendemen dan Profil Kimiawi Minyak Gaharu Aquilaria Sp.

Defiza,

Az Zahra,

Supandi

et al. 2023

Higea

View full text Add to dashboard Cite

Minyak gaharu salah satu produk hasil penyulingan dari resin gaharu yang selain aromanya yang unik dan memiliki aktivitas biologis. Oleh karena tingginya minat serta kelangkaan sumber-sumbernya secara alamiah, peneliti berusaha menemukan cara agar proses penyulingannya lebih efisien tanpa mengorbankan kualitas. Pada penelitian ini serbuk resin gaharu diberikan pretreatmen sebelum disuling dengan menggunakan microwave dengan daya 180 Watt selama 3 x 3 menit. Selanjutnya serbuk resin tersebut direndam masing-masing 1, 2, 3, dan 4 minggu sebelum dilakukan hidrodistilasi selama 15 jam. Minyak atsiri yang dihasilkan selanjutnya dianalisa menggunakan GCMS, sedangkan aromanya diuji hedonis untuk mengetahui perlakuan mana yang paling disukai responden. Hasil penelitian menunjukkan bahwa terjadi peningkatan rendemen minyak atsiri setelah pretreatment. Ada senyawa yang konsisten muncul pada semua perlakuan termasuk kontrol seperti benzylacetone, β-selinene, alloaromadendrene, n-hexadecanoic acid, β-guaiene, dan agarospirol, sedangkan senyawa yang muncul setelah diberikan perlakuan microwave di antaranya adalah di antaranya adalah γ-gurjunene, valerenol, valencene, dibenzylacetone, α-selinene, α-guaiene, beta elemene, γ-muurolene, isocaryophyllene, ocimene, valerenal, cadinene dan dimetil octatriene. Adapun di antara senyawa di atas, yang baru muncul setelah mengalami perendaman adalah valerenal, dibenzylacetone, isocaryophyllene, ocimene, α-guaiene, β-elemene, dan γ-muurolene. Praperlakuan dengan menggunakan microwave dengan daya 180W dan perendaman sampai 2 minggu dapat meningkatkan jumlah rendemen dan keanekaragaman komponen minyak gaharu atsiri. Uji hedonik menunjukkan responden aroma minyak atsiri setelah pretreatment tidak merusak kualitas minyak gaharu.

show abstract

“…Pers. (Arecaceae), a palm called 'Coco de Mer', contains high amounts of acyclic, bicyclic and monocyclic sesquiterpenoids (β-caryophyllene, bicyclogermacrene) and monoterpenes, known for their antimicrobial properties [34].…”

Section: In Silico Effectiveness Of Essential Oil Constituentsmentioning

confidence: 99%

Can Essential Oils Provide an Alternative Adjuvant Therapy for COVID-19 Infections and Pain Management at the Same Time?

2022

View full text Add to dashboard Cite

The active compounds from essential oils have been an important asset in treating different diseases for many centuries. Nowadays, there are various available formulations used as food supplements to stimulate the immune system. In light of the current pandemic and the large amount of fake news circulating the internet, it is important to analyze which of the active compounds from essential oils can be successfully used in the treatment of COVID-19 infections. We analyzed the current literature on the effects of essential oils against the new SARS-CoV-2 virus to gain a better understanding of the underlying mechanisms of these compounds and establish their possible antiviral efficacy. The available studies have highlighted the antiviral potential of active compounds from essential oils, indicating that they could be used as adjuvants in treating various viral infections, including COVID-19, leading to a milder course of the disease, and improving patients’ outcomes. At the same time, these compounds relieve pain and lift the mood in comorbid patients suffering from opioid addiction. Essential oils might be useful as adjuvant tools, not only against SARS-CoV-2 but also for a subset of especially vulnerable patients affected with both COVID-19 and opioid addiction. However, randomized clinical trials are needed to determine their efficacy and develop standardized high-quality preparations that can be safely administered to the general population.

show abstract

Anatomical Investigation and GC/MS Analysis of ‘Coco de Mer’,Lodoicea maldivica(Arecaceae)

Cited by 10 publications

References 56 publications

Ultrastructure of Terpene and Polyphenol Synthesis in the Bark of Cupressus sempervirens After Seiridium cardinale Infection

Ultrastructure of Terpene and Polyphenol Synthesis in the Bark of Cupressus sempervirens After Seiridium cardinale Infection

Pengaruh Pretreatment Microwave Terhadap Rendemen dan Profil Kimiawi Minyak Gaharu Aquilaria Sp.

Can Essential Oils Provide an Alternative Adjuvant Therapy for COVID-19 Infections and Pain Management at the Same Time?

Contact Info

Product

Resources

About