The purpose of this study is to provide anatomical information of BL40 including applicable needling depth, bloodletting methods and related physiological effect. Methods: The contents about BL40 were extracted from literature. Then we investigated the clinical effect, needling feature (depth/angle/stimulation method) and the bloodletting methods of BL40, searching online databases like 'RISS', 'Oasis', 'DBPIA', 'Naver Academic information', 'National Research institute of Korea', 'Pubmed', 'Google scholar' and 'CNKI' from 1990 to 2023. Results: According to the classic test, the needling depth of BL40 is 9.35-33.15 mm, that of previous research is 9.4 mm-23.4 mm. When acupuncture is applied at that level, stimulatable anatomical structures were deep popliteal fascia and tibial nerve.
Conclusion:The remarkable effect of BL40 is originated from stimulation of deep popliteal fascia and tibial nerve. It is necessary for BL40 to be stimulated as 15.5 mm depth.