Anatomical Distribution of Lipids in Human Brain Cortex by Imaging Mass Spectrometry

Veloso, António; Astigarraga, Egoitz; Barreda-Gómez, Gabriel; Manuel, Iván; Ferrer, Isidró; Giralt, M.T.; Ochoa, Begoña; Fresnedo, Olatz; Rodrı́guez-Puertas, Rafael; Fernández, José A.

doi:10.1007/s13361-010-0024-5

Cited by 46 publications

(43 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Emaitza hoberenak bi matrize desberdinetan lortzen dira erreflektroi moduaren arabera: 9AA eta MBT matrizeetan hain zuzen ere. Zenbait ikerketa egin dira matrize hauen eraginkortasuna egiaztatzeko lipidoen analisian [17][18][19][20]. 4. irudian, 9AA erabilita egiaztatzen da lipido batzuk ondo detektatzen direla RN moduan, baina masa tarte finko eta oso txikian.…”

Section: Matrizearen Aukeraketaunclassified

Lipidoak aztertu eta identifikatzeko TLC kromatografiaren eta MALDI masa-espektrometriaren arteko akoplamendua

Veloso¹

2018

EKAIA

Self Cite

View full text Add to dashboard Cite

Laburpena: Geruza meheko kromatografia teknika (TLC, ingelesezko Thin Layer Chromatography) oso erabilia izan da hainbat molekularen banaketa eta karakterizaziorako. Bestalde, Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) edo matrizez lagundutako laserraren bidezko desor tzioionizazioa/hegaldi-denbora masa-espektrometria teknikaren erabilera gero eta gehiago hedatzen ari da molekula desberdinen analisia egiteko. Gainera, Imaging Mass Spectrometry (IMS) edo masa-espektrometriaren bidezko irudia izeneko teknikak, ahalbidetzen du ehun biologikoetan dauden lipidoen, proteinen eta farmakoen banaketa-irudiak lortzea eta teknika bikaina da molekula horien banaketa aztertzeko. Teknika honek lipido mota ezberdinen identifikazioa eta lokalizazioa ahalbidetzen du, konposatuaren edota familia kimikoaren aukera egin aurretik. Analizatu behar diren konposatuen aukera aldez aurretik egin behar ez denez, aukera dago irudiak sortzeko masa-espektrometroan detektatutako ioietatik abiatuta. Lan honetan, erakusten da lipidoen ikerkuntzarako oso lagungarria dela TLC xaflak eta MALDI IMS teknikak konbinatzea.

show abstract

Section: Matrizearen Aukeraketaunclassified

Lipidoak aztertu eta identifikatzeko TLC kromatografiaren eta MALDI masa-espektrometriaren arteko akoplamendua

Veloso¹

2018

EKAIA

Self Cite

View full text Add to dashboard Cite

show abstract

“…288,294 Extracts can be stored frozen at -80°C until analysis. 287 Different lipid subtypes can be successfully quantified by using mass spectrometric detection coupled with either gas, liquid, or thinlayer chromatography. 108,222,223,295 Tandem mass spectrometry with a lipid database 296 can be used to discriminate among chemical lipid variants with identical masses due to the identical number of acylic carbons and double bonds.…”

Section: Lipid Isolationmentioning

confidence: 99%

“…108,222,223,295 Tandem mass spectrometry with a lipid database 296 can be used to discriminate among chemical lipid variants with identical masses due to the identical number of acylic carbons and double bonds. 287 Matrix-assisted laser desorption/ ionization mass spectrometry permits the direct scanning of tissue slides, and it thus identifies the precise localization of different lipids in the tissue. 297…”

Section: Lipid Isolationmentioning

confidence: 99%

“…• Proteins should be extracted from fresh or snap frozen material at -70°C or below 184,210,234 • Lipids should be extracted from fresh or snap frozen material at -20°C 108 or -80°C 223,287 Time to freeze • Freeze immediately after isolation. Samples derived postmortem should be frozen (at -80°C) short after death (maximum 2 h) for optimal results in protein studies and to avoid protein degradation 210,234 • Freeze immediately after isolation.…”

Section: Parametermentioning

confidence: 99%

See 1 more Smart Citation

Translational Research in Pediatrics IV: Solid Tissue Collection and Processing

2016

View full text Add to dashboard Cite

Solid tissues are critical for child-health research. Specimens are commonly obtained at the time of biopsy/surgery or postmortem. Research tissues can also be obtained at the time of organ retrieval for donation or from tissue that would otherwise have been discarded. Navigating the ethics of solid tissue collection from children is challenging, and optimal handling practices are imperative to maximize tissue quality. Fresh biopsy/surgical specimens can be affected by a variety of factors, including age, gender, BMI, relative humidity, freeze/thaw steps, and tissue fixation solutions. Postmortem tissues are also vulnerable to agonal factors, body storage temperature, and postmortem intervals. Nonoptimal tissue handling practices result in nucleotide degradation, decreased protein stability, artificial posttranslational protein modifications, and altered lipid concentrations. Tissue pH and tryptophan levels are 2 methods to judge the quality of solid tissue collected for research purposes; however, the RNA integrity number, together with analyses of housekeeping genes, is the new standard. A comprehensive clinical data set accompanying all tissue samples is imperative. In this review, we examined: the ethical standards relating to solid tissue procurement from children; potential sources of solid tissues; optimal practices for solid tissue processing, handling, and storage; and reliable markers of solid tissue quality.

show abstract

“…Lipids are perhaps the most widely-studied class of compounds with MALDI imaging, both in the MS [12][13][14][15][16][17] and MS/MS 18 modes.…”

Section: Introductionmentioning

confidence: 99%

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Han

Borchers

2013

JoVE

View full text Add to dashboard Cite

Mass spectrometry imaging (MSI) determines the spatial localization and distribution patterns of compounds on the surface of a tissue section, mainly using MALDI (matrix assisted laser desorption/ionization)-based analytical techniques. New matrices for small-molecule MSI, which can improve the analysis of low-molecular weight (MW) compounds, are needed. These matrices should provide increased analyte signals while decreasing MALDI background signals. In addition, the use of ultrahigh-resolution instruments, such as Fourier transform ion cyclotron resonance (FTICR) mass spectrometers, has the ability to resolve analyte signals from matrix signals, and this can partially overcome many problems associated with the background originating from the MALDI matrix. The reduction in the intensities of the metastable matrix clusters by FTICR MS can also help to overcome some of the interferences associated with matrix peaks on other instruments. High-resolution instruments such as the FTICR mass spectrometers are advantageous as they can produce distribution patterns of many compounds simultaneously while still providing confidence in chemical identifications. Dithranol (DT; 1,8-dihydroxy-9,10-dihydroanthracen-9-one) has previously been reported as a MALDI matrix for tissue imaging. In this work, a protocol for the use of DT for MALDI imaging of endogenous lipids from the surfaces of mammalian tissue sections, by positive-ion MALDI-MS, on an ultrahigh-resolution hybrid quadrupole FTICR instrument has been provided. Video LinkThe video component of this article can be found at

show abstract

Anatomical Distribution of Lipids in Human Brain Cortex by Imaging Mass Spectrometry

Cited by 46 publications

References 33 publications

Lipidoak aztertu eta identifikatzeko TLC kromatografiaren eta MALDI masa-espektrometriaren arteko akoplamendua

Lipidoak aztertu eta identifikatzeko TLC kromatografiaren eta MALDI masa-espektrometriaren arteko akoplamendua

Translational Research in Pediatrics IV: Solid Tissue Collection and Processing

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Contact Info

Product

Resources

About