African catfish is believed to be native of Africa with a wide geographical distribution within the continent. It belongs to the family of Claridae consisting of about 14 genera, 5 subgenera and 32 species including Clarias, Clarioides, Anguillo, Platycephaloides and Brevicephaloides [1, 2, 3]. Clarias gariepinus is one of the most suitable species for aquaculture in Africa attaining about a kg body weight at about 6 months of age especially under intensive management conditions. It was reported that C. gariepinus was hardy and adapted to diverse environments even with poor water quality [4]. It is highly cherished by many Nigerians, hence it commands very high commercial value and it is the most popular cultured fish in the country though still at subsistence level. Unfortunately, C. gariepinus does not breed in captive hence, some artificial breeding approaches [5, 6 and 7]. Meanwhile, C. gariepinus eggs, larvae, fry and fingerlings could be collected from natural environment for stocking but may not be available all-year-round [8, 9, 10, and 11]. Thus, it was suggested [12] that systematic pond propagation method would ensure fish production all-year-round. In this method, the female is injected with an appropriate hormone to facilitate oocyte development and egg maturation prior to spawning. Regrettably, the male must be killed to obtain the milt resulting in scarcity of male brood stock. In some cases, the milt is harvested from many males depending on the commercial status of the farm yet, either the harvested milt is not enough warranting killing of more males or is in excess of what is needed. This often led to colossal losses. At the moment, there is little or no information on alternative means of reducing this colossal wastage except, the use of syringe attempted by [13], cryopreservation technique propounded by [14] and Abstract: African catfish (C. gariepinus) does not reproduce in captive, hence the adoption of artificial breeding strategy. This study was targeted at the possibility of storing milt at different temperatures, to ensure all-year-round breeding operation. To accomplish this aim, milt was harvested from a total of twelve African catfish and stored at three different temperatures designated as treatments T1 (26.3 o C): Bench temperature (control: without refrigeration), T2 (7.42 o C): Fridge temperature and T3 (1.3 o C): Freeze temperature. Results indicated that the room and testes temperature values were constantly 36.4 o C and 26.0 0 C, standard body and total body length values ranged from 42 to 43 cm and 48 to 50 cm respectively. While the papilla length value varied from 1.5 to 1.8 cm, the fin length value was between 8.5 and 13.3 cm. The sperm cells concentration value was as high as 5.62-6.54 x 10 9 across the treatments and the alive sperm cells were up to 75-79% at 0 minute but deteriorated to as low as 1.5-4% at 15 minutes. Though some alive and motile sperm cells were observed at 1 hour, the percentage was as low as 0.25%, suggesting that catfish milt stored without cryoprese...