Anatomical, biochemical and morphological responses of poplar Populus deltoides clone Lux to Zn excess

Stoláriková-Vaculíková, Miroslava; Romeo, Stefania; Minnocci, A.; Luxová, Miroslava; Vaculík, Marek; Lee, Alexander; Sebastiani, L.

doi:10.1016/j.envexpbot.2014.07.001

Cited by 39 publications

(13 citation statements)

References 37 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that heavy metals can affect root and leaf anatomy. For example, elevated concentration of Zn was shown to modify the leaf thickness and increase intercellular space area in poplar exposed to Zn excess (Todeschini et al 2011;Stoláriková-Vaculíková et al 2015). Similarly, Luković et al (2012) observed reduction of stomatal size, adaxial and abaxial epidermis, and percentage of spongy tissue thickness in poplars and willows treated by Cd; and Zarinkamar et al (2013) and Queiroz Alves et al (2014) observed anatomical differences in leaves exposed to Pb.…”

Section: Discussionmentioning

confidence: 90%

Antimony (SbIII) reduces growth, declines photosynthesis, and modifies leaf tissue anatomy in sunflower (Helianthus annuus L.)

Vaculík

Mrázová

Lee

2015

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

The role of antimony (Sb)--a non-essential trace metalloid--in physiological processes running in crops is still poorly understood. Present paper describes the effect of Sb tartrate (SbIII) on growth, Sb uptake, photosynthesis, photosynthetic pigments, and leaf tissue organization in young sunflower plants grown in hydroponics. We found that growth of below- and aboveground part was reduced with increasing concentration of Sb in the medium. Although Sb was mostly taken up by sunflower roots and only small part (1-2%) was translocated to the shoots, decline in photosynthesis, transpiration, and decreased content of photosynthetic pigments were observed. This indicates that despite relatively low mobility of Sb in root-shoot system, Sb in shoot noticeably modifies physiological status and reduced plant growth. Additionally, leaf anatomical changes indicated that Sb reduced the size of intercellular spaces and made leaf tissue more compact.

show abstract

Section: Discussionmentioning

confidence: 90%

Antimony (SbIII) reduces growth, declines photosynthesis, and modifies leaf tissue anatomy in sunflower (Helianthus annuus L.)

Vaculík

Mrázová

Lee

2015

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…In spite of structural and ultrastructural changes to leaf lamina, photosynthetic balance and biomass production in P. deltoides might be sustained by the fact that photosynthetic pigment concentrations remain unchanged under heavy metal stress (Stoláriková-Vaculíková et al 2015). Thicknesses of the spongy and palisade parenchyma are associated with biomass production and could be used as indicators of growth potential in poplars (Al Afas et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Assessment of cadmium tolerance and phytoextraction ability in young Populus deltoides L. and Populus × euramericana plants through morpho-anatomical and physiological responses to growth in cadmium enriched soil

Nikolić¹,

Zorić²,

Cvetković³

et al. 2017

iForest

View full text Add to dashboard Cite

(2)Fast growing woody plants represent effective tools for cadmium (Cd) extraction during remediation of low to medium Cd contaminated soils. Poplars are good candidates for this task because of their rapid growth rate, high biomass yield, and adaptability, as well as the availability of well-characterized clones/ genotypes with various anatomical and physiological traits. The present study evaluates the potential of Populus deltoides (clone B-81) and Populus × euramericana (clone Pannonia) for phytoremediation of Cd contamination in soil. Poplar clones were analyzed for (1) plant growth response to Cd contamination, (2) Cd accumulation, translocation, and partitioning between plant organs, and (3) morphological, anatomical and physiological responses to Cd stress as a function of biomass production. Plants were cultivated in soil moderately contaminated with Cd (8.14 mg kg -1 soil) under semi-controlled conditions for six weeks. Our results suggest that P. × euramericana and P. deltoides clones respond differently to Cd contamination. Biomass production and morphological characteristics were more negatively affected in P. × euramericana than in P. deltoides plants. However, most examined leaf structural parameters were not significantly affected by Cd. In most cases, photosynthetic characteristics and gas exchange parameters were affected by Cd treatment, but the levels and patterns of changes depended on the clone. High tolerance to applied Cd levels, as estimated by the tolerance index, was observed in both clones, but was higher in P. deltoides than P. × euramericana (82.2 vs. 66.5, respectively). We suspect that the higher tolerance to Cd toxicity observed in P. deltoides could be related to unchanged proline content and undisturbed nitrogen metabolism. Following treatment, 58.0 and 46.7% of the total Cd content was accumulated in the roots of P. × euramericana and P. deltoides, respectively, with the remainder in the stems (18.2 and 39.9%) and leaves (23.8 and 13.4%). In summary, P. deltoides displayed better phytoextraction performance under Cd exposure than P. × euramericana, suggesting its potential not only for Cd phytostabilization, but also phytoextraction projects.

show abstract

“…Similar to H. vulgare [ 40 ] and P. × euramericana [ 21 ] but different from P. deltoides [ 22 ], an exodermis (i.e. hypodermis with Casparian bands; [ 75 ]) was never observed with P. × canescens roots cultivated in hydroponic control conditions.…”

Section: Discussionmentioning

confidence: 99%

“…However, it has been discussed before that either method on its own is not sufficient to reliably elucidate the complex structures, properties, and interactions of suberized transport barriers in roots [ 5 , 34 ]. It is especially the combination of protocols for consistent plant cultivation, histochemistry, biochemical investigation, transcriptomics, and final functional studies investigating transport physiology that will enable the best comparability to previously published research [ 21 , 22 , 35 – 47 ].…”

Section: Introductionmentioning

confidence: 99%

Hydroponic cultivation conditions allowing the reproducible investigation of poplar root suberization and water transport

Grünhofer

Guo

et al. 2021

Plant Methods

View full text Add to dashboard Cite

Background With increasing joint research cooperation on national and international levels, there is a high need for harmonized and reproducible cultivation conditions and experimental protocols in order to ensure the best comparability and reliability of acquired data. As a result, not only comparisons of findings of different laboratories working with the same species but also of entirely different species would be facilitated. As Populus is becoming an increasingly important genus in modern science and agroforestry, the integration of findings with previously gained knowledge of other crop species is of high significance. Results To ease and ensure the comparability of investigations of root suberization and water transport, on a high degree of methodological reproducibility, we set up a hydroponics-based experimental pipeline. This includes plant cultivation, root histochemistry, analytical investigation, and root water transport measurement. A 5-week-long hydroponic cultivation period including an optional final week of stress application resulted in a highly consistent poplar root development. The poplar roots were of conical geometry and exhibited a typical Casparian band development with subsequent continuously increasing suberization of the endodermis. Poplar root suberin was composed of the most frequently described suberin substance classes, but also high amounts of benzoic acid derivatives could be identified. Root transport physiology experiments revealed that poplar roots in this developmental stage have a two- to tenfold higher hydrostatic than osmotic hydraulic conductivity. Lastly, the hydroponic cultivation allowed the application of gradually defined osmotic stress conditions illustrating the precise adjustability of hydroponic experiments as well as the previously reported sensitivity of poplar plants to water deficits. Conclusions By maintaining a high degree of harmonization, we were able to compare our results to previously published data on root suberization and water transport of barley and other crop species. Regarding hydroponic poplar cultivation, we enabled high reliability, reproducibility, and comparability for future experiments. In contrast to abiotic stress conditions applied during axenic tissue culture cultivation, this experimental pipeline offers great advantages including the growth of roots in the dark, easy access to root systems before, during, and after stress conditions, and the more accurate definition of the developmental stages of the roots.

show abstract

Anatomical, biochemical and morphological responses of poplar Populus deltoides clone Lux to Zn excess

Cited by 39 publications

References 37 publications

Antimony (SbIII) reduces growth, declines photosynthesis, and modifies leaf tissue anatomy in sunflower (Helianthus annuus L.)

Antimony (SbIII) reduces growth, declines photosynthesis, and modifies leaf tissue anatomy in sunflower (Helianthus annuus L.)

Assessment of cadmium tolerance and phytoextraction ability in young Populus deltoides L. and Populus × euramericana plants through morpho-anatomical and physiological responses to growth in cadmium enriched soil

Hydroponic cultivation conditions allowing the reproducible investigation of poplar root suberization and water transport

Contact Info

Product

Resources

About